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- Long-term,multi-dimensional livecell imaging
Long-term,multi-dimensional livecell imaging
| Long-term, Six-dimensional Live-cell Imaging Kazuo Yamagata Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN-Kobe IVF embryos were injected with a mixture of mRNAs for EGFP-α -tublin(Tublin,green) and H2B-mRFP1(H2B,red).The embryos were imaged by being exposed to two different wavelength of excitation(488 and 561 nm) at 7.5min intervals for about 70h and 51 images were acqired in the Z axis at each time point( total of 56,814 fluorescent images). As result of transplanting the embryos after imaging , all the pups were healthy, reproductively normal and not transgenic. Thus, this live-cell imaging technology is safe for full-term mouse development. IVF:in vitro fretilization |
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Left：DIC Right：Confocal Tubulin：Green （EGFP- α -tubulin） Null：Red （H2B-mRFP1） movie(size:73,152kb) |
