Long-term,multi-dimensional livecell imaging
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Long-term, Six-dimensional Live-cell Imaging
Kazuo Yamagata
Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN-Kobe
IVF embryos were injected with a mixture of mRNAs for EGFP-α-tublin(Tublin,green) and H2B-mRFP1(H2B,red).The embryos were imaged by being exposed to two different wavelength of excitation(488 and 561 nm) at 7.5min intervals for about 70h and 51 images were acqired in the Z axis at each time point( total of 56,814 fluorescent images).
As result of transplanting the embryos after imaging , all the pups were healthy, reproductively normal and not transgenic.
Thus, this live-cell imaging technology is safe for full-term mouse development.
IVF:in vitro fretilization |
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Left：DIC
Right：Confocal
Tubulin：Green
（EGFP- α -tubulin）
H2B:Red
（H2B-mRFP1）
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(movie size:73,152kb) |
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