Real-time/real-color FRET imaging/Yellow Cameleon (in Japanese)
Takeharu Nagai, and Atsushi Miyawaki
A real-time movie of confocal real color images and corresponding pseudocolored ratio images showing Ca2+ waves inside cells, which were evoked by histamine, in HeLa cells expressing YC3.60(Yellow Cameleon). The images were taken at video rate (30 Hz) using a color 3CCD camera for simultaneous acquisition of CFP (cyan-emitting mutant of GFP) and YFP (yellow-emitting mutant of GFP) images. To improve z axis resolution, a spinning disk confocal unit was placed in front of the camera. Ten micromolar histamine was added to the recording medium to activate receptor-evoked Ca2+ release from endoplasmic reticulum.
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