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Real time calcium imaging
| *Movies from Tetsuro Takamatsu’s laboratory (In Japanese) Real-time intracellular calcium dynamics of the Purkinje fibers in the perfused rat heart The fluo3-fluorescence (8 bits, pseudo-color image) of the perfused rat heart visualized by the CSU-based real-time confocal microscopy at 30 frames/s. Pixels 205 x 230. The scale bar denotes 50 μm. Microscope: BX50WI, Olympus、Objective lens: LUMIPlan FL x20, Olympus, with CSU-21, CCD camera: ES310 Turbo, Roper Scientific & Image intensifier: VS4-1845, Videoscope. |
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Spatially uniform Ca2+ transients are identified in Purkinje fibers on the ventricular septal wall. play (movie size :3,731kb) |
| Simultaneous imaging of phosphatidyl inositol metabolism and Ca2+ levels in PC12h cells
BBRC 308 (2003) 673-678 Mitsuhiro Morita, Fumito Yoshiki, and Yoshihisa Kudo Laboratory of Cellular Neurobiology, School of Life Science, Tokyo University of Pharmacy and Life Science (in Japanese) Relationship between phosphatidyl inositol metabolism and Ca 2+ level was analyzed with high spatial resolution at video rate in PC12h cells double-labeled with GFP fused with the pleckstrin homology domain and FuraRed, CSU10, C6790 CCD camera and W-View; simultaneous dual color imaging system (Hamamatsu), Nikon E600FN microscope with Fluor 40X/0.8W. |
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Left : EGFP Right : FraRed play (movie size :3,731kb) |
| Functional multineuron calcium imaging Yuji Ikegaya Laboratory of Chemical Pharmacology, Graduate school of Pharmaceutical Sciences, The University of Tokyo. fMCI is a functional imaging technique with multicell loading of calcium fluorophores. fMCI has unique advantages, including: i) recording en masse from hundreds of neurons in a wide area, ii) single-cell resolution, iii) identifiable location of neurons, and iv) detection of non-active neurons during the observation period |
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Spontaneous firing-induced somatic calcium spikes of CA3 pyramidal cells in a rat hippocampal slice culture loaded with Oregon Green 488 BAPTA-1AM play (movie size :4,530kb) |
