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Long-time, 4 colors, and 3D in vivo imaging.
Mikala Egeblad , Andrew Ewald, Zena Werb.
UCSF, Dept of Anatomy
Researchers at UCSF developed in vivo imaging system capable of multicolor, 3D imaging at multi-points to observe tumor microenvironment in the same live mouse for long time, by using the CSU.
They have successfully tracked dynamics of several types of stromal cells in different tumor microenvironment for up to 12 hours to find different behaviors of each stromal cell, which could lead to useful insight to define the contributions of specific stromal cells to tumor growth.
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Green：c-fms-EGFP Blue：ACTB-ECFP
Red：10 kDa dextran
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Intravital Molecular Imaging
Satoshi Nishimura
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo
Dr. Satoshi Nishimura is a world-renowned expert of intravital molecular imaging; which means in vivo imaging of microcirculation and molecular dynamics in living animals with high spatiotemporal resolution.
Intravital Molecular imaging is a powerful tool to elucidate not only vascular pathological conditions such as arteriosclerosis, but also to study molecular mechanisms of pathological conditions caused by complicated and multi-cellular abnormal interactions, such as cancer or metabolic syndrome.
He has developed imaging system based on highly spatiotemporal imaging with the CSU and visualized microcirculation, vascular functions and multi-cellular dynamics of target molecules in real-time and in threedimensions, in vivo.
Using this technique, he successfully elucidated how obese adipose tissue played a bad role in obesity.
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An example of real-time movie of microcirculation in mouse, which clearly shows dynamic movement and interactions among leucocytes, platelets, macrophages and endothelium.
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