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Cell Division -3
| Movies by Paul
Maddox, Ludwig Inst. of Cancer Research Time-lapse images were acquired by using a CSU10 Yokogawa spinning-disk confocal system (Perkin-Elmer Life Sciences Wallac) mounted on a Nikon Eclipse TE300 inverted microscope (Nikon Instrument Group). The specimens were illuminated at 488 nm with an air-cooled Ar/Kr laser (60mW, Melles Griot). Digital images were acquired with an Orca ER,Hamamatsu 16-bit cooled CCD camera (Hamamatsu Photonics), providing the goa-1 and gpa-16 DNA constructs, Stephan Grill and the acquisition system was controlled by MetaMorph software (Universal Imaging). Fluorescence images were acquired with 350 ms exposure at 2- or 5-s intervals by using a Plan Apochromat 100_/1.4 NA objective and 2 _ 2 binning in the camera. Images were analyzed by using MetaMorph software and Microsoft Excel (Microsoft) and were processed with Adobe Photoshop (Adobe Systems) |
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Drosophila embryo expressing GFP-centromere protein & injected with X-rhodamine tubulin , Maddox et.al. Current Biology(2002) play (movie size :770kb) |
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GFP-Histone & GFP spindle poles in C. elegans embryo. play (movie size :803kb) |
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GFP tubulin in C. elegans embryo. play (movie size :405kb) |
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Hela_yfp_cenpa_control1 : HeLa cell expressing YFP-CENP-A(kinetochore specific protein) with DIC. play (movie size :575kb) |
