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CSU22
4.Real time 3D Observation
 
In synchronization with fast objective lens movement using piezo-actuator and a high-speed camera, CSU22 enables you to perform high-speed 3D scanning of 30 or more cross-sectional images per second .
 
Example of Application
 
3D Reconstruction of Moving Objects
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3D tracking of the behavior of C.elegans expressing EGFP-fused pharyngeal protein (3D images were reconstructed after acquiring each 30 cross section images per second)
C.Elegans1 C.Elegans2 C.Elegans3
1sec 2sec 3sec
 
Real time 3D imaging of signal transduction
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Single section confocal images
3d1 3d2 3d3
0 sec 6 sec 12 sec
 
Projection ( overlay ) of 30 cross-sectional images per second
3d4 3d5 3d6
High-speed 3D scanning of glutamic acid-stimulated Ca2+ reactions in cerebral cortical nerve cells labeled with Fluo-3

By courtesy of Dr. Atsuo Fukuda , Dept. of Physiology ,Hamamatsu University School of Medicine.
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