Cycle et science de vie

 

Our microscopy and life sciences solutions are designed to support applications from basic research to drug discovery to pre-clinical trials. Yokogawa’s high content analysis systems and dual spinning disk confocal technologies are used in regenerative medicine, drug discovery, and precision medicine, providing high-speed and high-resolution live cell imaging.。
 

 

  • FlowCam: Flow Imaging Microscopy

    With the FlowCam you can analyze particles accurately, reliably and quickly using automated imaging technology to advance your research, increase productivity, and ensure quality.

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  • OpreX Informatics Manager

    Yokogawa Electric's OpreX Informatics Manager is an information-integrated solution that goes beyond electronic lab notebooks by optimizing human and material resource management in terms of skills and scheduling.

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Details

July 27,2018

Sales release : High-speed Super resolution Confocal Scanner CSU-W1 SoRa

 Click Here For More Info

June 11,2018

2018 SLAS Europe

March 01,2018

Sales release : High Content Data Management System CellLibrarian

 Click Here For More Info

December 29,2017

SLAS 2018

February 3-7, 2018

Find out more about SLAS 2018

December 29,2017 Sales news : The Discontinuation of CellVoyagerTM CV7000S High-throughput Cytological Discovery System
September 05,2017

Sales release : CellVoyagerTMCV8000 High-throughput Cytological Discovery System

 Click Here For More Info

January 19,2017

SLAS High-Content Screening Conference 2017

Find out more about SLAS High-Content Screening Conference 2017

April 04,2016

Poster presentation in 3D Cell Culture 2016, 19-21 April 2016, Konzerthaus Freiburg/Germany

Yokogawa Electric Corporation will present data obtained by our confocal image cytometer CQ1 in “3D Cell Culture 2016: How close to ‘in vivo’ can we get? Models, Application & Translation”. The poster will show the results of 3D live cell imaging and analysis of the migration and the network formation of HUVEC cells in a multilayered cell sheet. The results demonstrate that CQ1 is an excellent research tool in the field such as regenerative medicine and drug discovery screening.

*Data were provided from BioProcess Systems Engineering Lab., Dept.Biotech., Grad. Sch. Eng., Osaka University.

Poster presentation in 3D Cell Culture 2016

February 10,2016 Yokogawa Concludes Distribution Agreement with Optec, LLC for Sale of Confocal Quantitative Image Cytometer CQ1 at the markets of OPTEC activity
October 01,2015 Sales release : Label-free Morphological Analysis Software CellActivision

 

Principles of Spinning disk confocal

The most common conventional confocal microscopes use a single laser beam to scan a specimen, while the CSU scans the field of view with approximately 1,000 laser beams, by using microlens-enhanced Nipkow-disk scanning: in short, CSU can scan 1,000 times faster.

By using a disk containing microlens arrays in combination with the Nipkow disk, we have succeeded in dramatically improving the light efficiency and thus successfully made real-time confocal imaging of live cells possible.

The expanded and collimated laser beam illuminates the upper disk containing about 20,000 microlenses (microlens array disk). Each microlens focuses the laser beam onto its corresponding pinhole, thus, effectively increasing laser intensity through pinholes placed in the pinhole array disk (Nipkow disk).

With the microlens, backscattering of laser light at the surface of the pinhole disk can be significantly reduced, thus, dramatically increasing the signal to noise ratio (S/N) of confocal images.

About 1,000 laser beams passing through each of the pinholes fill the aperture of the objective lens, and are then focused on the focal plane. Fluorescence generated from the specimen is captured by the objective lens and focused back onto the pinhole disk, transmitted through the same holes to eliminate out-of-focus signals, deflected by the dichroic mirror located between microlens array disk and the Nipkow disk to split fluorescence signal from reflected laser, passed through emission filter and then focused into the image plane in the eyepiece or camera.

The microlens array disk and the Nipkow disk are physically fixed to each other and are rotated to scan the entire field of view at high speeds, thus, making it possible to view confocal fluorescent images in real-time through the eyepiece of the CSU head.

As compared to conventional single point scanning, multi beam scanning by the CSU requires a significantly low level of light intensity per unit area, which results in significantly reduced photo bleaching and photo toxicity in live cells.

Spinning disk confocal

microlens / fastsacnning / minimal photo bleach / high resolution

Microlens-enhanced Nipkow Disk Technology

Microlens-enhanced Nipkow Disk Technology

Comparison of scanning method

point scanning

Point Scanning
1 line scan time=1[ms]
1000 lines/image
Scan lines=1000 [lines]
1×1000=1000 [ms]

disk scanning

Disk Scanning by CSU
Rotation Speed=10000 [rpm]=41.7[rps]
30°Rotation/image
1÷( 41.7×30/360 )= 0.5 [ms]

 

 

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Ressources

Vue générale:

Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression

Vue générale:

Faster, Deeper, and Clearer -in vivo molecular imaging technology-

Vue générale:

Discovering the Basic Principles of Life through the Live Imaging of C. elegans

Vue générale:

Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.

Vue générale:

New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!

Vue générale:

Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.

Vue générale:

Use of the spinning disk confocal at the Harvard Medical School microscopy core.

Vue générale:

Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.

Vue générale:

On-site manipulation of protein activities: Understanding intricate cell signaling pathways.

Notes Applicatives
Vue générale:

Long-term observation of mitosis by live-cell microscopy is required for uncovering the role of Cohesin on compartmentalized nuclear architecture which is linked to nuclear functions.
To perform long term observation of mitosis devices are needed that have low phototoxic effects on living cells and enable high speed imaging. By using the CSU W-1 confocal scanner unit for time lapse imaging entrance into mitosis, mitotic progression and exit can be examined.

Vue générale:

Comparison between CSU and conventional LSM in 4D movies.

Notes Applicatives
Vue générale:

A critical requirement in biopharmaceutical development is the integration and automation of process equipment and analytical instruments used in the laboratory. Bioprocess labs with multiple lab-scale bioreactors often execute cultivation experiments in parallel for research or process development purposes.

As part of a collaboration between Securecell (Zurich, SW) and Yokogawa Life Science (Tokyo, Japan), this application note demonstrates the effective use of the Lucullus® Process Information Management System (Lucullus®) to assist in the control of three Advanced Control Bioreactor Systems (BR1000) to study glucose utilization of CHO cells for optimal monoclonal antibody productivity.

Industries:
Notes Applicatives
Notes Applicatives
Vue générale:

To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

Notes Applicatives
Vue générale:

CV1000 clears the hurdle in Live Cell Imaging
All-in-one Live cell imaging solution

Notes Applicatives
Vue générale:

The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.

Notes Applicatives
Notes Applicatives
Vue générale:

Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.

Notes Applicatives
Vue générale:

The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

Notes Applicatives
Vue générale:

List of Selected Publications : CSU-W1

Vue générale:

List of Selected Publications : CQ1

Notes Applicatives
Vue générale:

List of Selected Publications : CSU-X1

Vue générale:

List of Selected Publications : CV8000, CV7000, CV6000

Notes Applicatives
Vue générale:
  • Colony Formation
  • Scratch Wound
  • Cytotoxicity
  • Neurite Outgrowth
  • Co-culture Analysis
  • Cell Tracking
Vue générale:

Faster, Brighter, and More Versatile Confocal Scanner Unit

Vue générale:

Fluorescent ubiquitination-based cell cycle indicator (Fucci) is a set of fluorescent probes which enables the visualization of cell cycle progression in living cells.

Notes Applicatives
Vue générale:

Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System

Vue générale:

This application note will introduce the features of the SU10 and provide examples demonstrating the delivery of genome editing tools (Cas9 RNP) using the technology.

Notes Applicatives
Vue générale:

Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer

Notes Applicatives
Vue générale:

Wide and Clear
Confocal Scanner Unit

Rapports techniques
2.2 MB
Vue générale:

This "Tutorial" provides overview of this software, from installation through data analysis.

Vue générale:

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

Vue générale:

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

Vue générale:

In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.

Vue générale:

In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

Vue générale:

In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.

Vue générale:

In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.

Vue générale:

In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.

Vue générale:

In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.

Vue générale:

In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.

Vue générale:

In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.

Vue générale:

In this tutorial, we’ll compare positive and negative condition for the count and total area of lipid droplets by adding the oleic acid or Triacsin C.

Downloads

Vidéos

Vue d’ensemble
Vue générale:

The Yokogawa business vision states that the company endeavors to achieve Net-zero emissions, ensure the Well-being of all, and make a transition to a Circular Economy by 2050. 

Vue générale:

YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.

Vue générale:

YOKOGAWA aspires to establish Smart GMP manufacturing facilities that provide consistent quality and supply while eliminating industrial waste, enhancing productivity and always using high-quality component parts and materials.

Vue générale:

YOKOGAWA creates autonomous operations with high-efficiency automation and optimization that allows growth with minimal deployment of manpower.

Vue générale:

In this webinar, Professor Jonny Sexton discusses a pipeline, developed in the Sexton lab, for the quantitative high-throughput image-based screening of SARS-CoV-2 infection to identify potential antiviral mechanisms and allow selection of appropriate drug combinations to treat COVID-19. This webinar presents evidence that morphological profiling can robustly identify new potential therapeutics against SARS-CoV-2 infection as well as drugs that potentially worsen COVID-19 outcomes.

Communiqués de presse

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