Benchtop System

CQ1은 3D 배양기 내의 회전 타원체와 같은 살아있는 셀 클러스터의 3D 이미징 및 정량화를 그대로 유지하여 셀을 손상시키지 않습니다. CQ1은 고급 데이터 분석을 위해 다양한 타사 소프트웨어에서 읽을 수 있는 일반 형식의 기능 데이터를 내보냅니다. 배양접시 조작을 위한 로봇을 통해 외부 시스템*1 과 통합하여 완전히 맞춤화된 CQ1 기반 시스템을 구축 할 수 있습니다.

CQ1 시스템 주요 특징

Excitation laser wavelength 405 nm, 488 nm, 561 nm, 640 nm
Illumination source Laser
Objective lens 2x to 60x
(Dry, Phase contrast, Long working distance)
Camera High-sensitivity sCMOS camera
Autofocus Laser autofocus, Software autofocus
Software CellPathfinder

회전 타원체, 콜로니 및 조직 단면 측정 가능

  • 기존의 유동 세포 계측법과 달리 배양 접시에서 세포를 제거 할 필요가 없습니다.
  • Nipkow 스피닝 디스크 공초점 기술로 고속의 부드러운 3D 이미지 획득이 가능합니다.
  • 풍부한 기능 추출로 정교한 셀룰러 이미지 분석이 용이합니다.
  • 넓은 시야 및 타일링 기능을 통해 대형 시료를 쉽게 촬영할 수 있습니다.

타임 랩스 및 라이브 셀 분석 가능

  • 고정밀 스테이지 인큐베이터와 공초점의 낮은 광독성으로 시간 추이와 살아있는 셀의 분석이 가능합니다.  
  • 고속 랩스*1 를 위한 Max.20fps 옵션 
CQ1

높은 품질의 이미지 및 전통적인 흐름 세포측정기(flow cytometer)와 유사한 조작성

  • 이미지 수집을 통해 실시간으로 표시되는 기능 데이터 및 통계 그래프
  • 공초점 현미경 이미지로 사용 가능한 고품질 이미지
  • 그래프 스팟에서 원래 이미지로 쉽게 돌아가고 반복 측정 가능

개방형 플랫폼

  • 핸들링 로봇

    Content...

    을 통해 외부 시스템과 연결 가능
  • 이미지 수집 및 정량화 도구를 이용하여 통합 시스템으로 확장 가능
  • 타사 데이터 분석 소프트웨어에서 읽을 수 있는 FCS/CSV/ICE 데이터 형식
  • 다양한 세포 배양 및 샘플 확인 가능

컴팩트한 설치 공간, 가벼운 무게의 벤치탑 장치, 암실 불필요

  CQ1 General fluorescent imaging Flow cytometry
Cell removal/suspension treatment Not necessary Not necessary Necessary
Cell image confirmation Possible Possible Not possible
Display feature data and graphs in real-time with imaging Possible Depends on devices Possible
3D data measurement Possible Not possible Not possible
Time lapse Possible Not possible Not possible

*1 옵션
*2 자세한 내용은 CQ1 담당자에게 문의하십시오.

Details

자세한 내용은 아래 SNS 계정에서 확인하십시오.

 

Yokogawa Life Science

•Twitter @Yokogawa_LS
•Facebook Yokogawa Life Science
•LinkedIn Yokogawa Life Science

 

Yokogawa 공식 소셜 미디어 계정 목록

Social Media Account List

Multiple functions fully integrated in a compact box

Compact design contains fully integrated multiple functions to offer easy-to-handle confocal imaging system, without a need for complicated system integration; you only need to set a sample and run the software. User-friendly interface and versatile functions support your measurement and analysis.

CQ1

Principles of the Microlens-enhanced Nipkow Disk Scanning Technology

A Nipkow spinning disk containing about 20,000 pinholes and a subsidiary spinning disk containing the same number of microlenses to focus excitation laser light into each corresponding pinhole are mechanically fixed on a motor, and very rapidly rotated. As a result, high-speed raster scan of the excitation lights on the specimen can be achieved.The pinhole and microlenses are arranged on each disk in our proprietary design to optimize raster scan. Multi-beam scanning not only increases scanning speed, but also results in significantly lower photobleaching and phototoxicity, because multi-beam excitation needs only low level of laser power on the specimen to fully excite fluorescence.

CQ1

System integration with CQ1

System integration with CQ1

Measurement Procedure

Analysis software (Option)

High content analysis system CellPathfinder*1        Click Here For More Info!

  • Preset analysis menus for a variety of applications
  • Flexible graph functions to display analysis results
  • Direct link between chart and object imageMachine learning

Machine learning


Software learns the features of the sampleobjects collected by users.

3D analysis


 

Label-free analysis
DPC*2 function is a powerful tool to analyze unstained bright field samples.

*1 Optional software
*2 Digital phase contrast

Example of setup

Example of setup

 

Item Specifications
Optics Microlens enhanced dual wide Nipkow disk confocal,
Phase contrast (Optional add-on)
Laser/Filter Laser : Choose 2-4 lasers from 405/488/561/640nm,
10-position Filter wheel (built-in)
Camera sCMOS 2560×2160pixel, 16.6×14.0mm
Objective lens Max.6 lenses
(Dry: 2x, 4x, 10x, 20x, 40x Long working distance: 20x, 40x
Phase contrast: 10x, 20x )
Sample vessel Microplate (6, 24, 96, 384 well), Slide glass,
Cover glass chamber*1, Dish (35, 60mm*1)
XY stage High-precision XY stage, designated resolution 0.1µm
Z focus Electric Z motor, designated resolution 0.1µm
Autofocus Laser autofocus, Software autofocus
Feature data Number of cells/cellular granules, Intensity, Volume, Surface area, Area, Perimeter, Diameter, Sphericity, Circularity, etc
Data format Image : 16bit TIFF file (OME-TIFF), PNG
Numerical data : FCS, CSV, ICE
Workstation Measurement and analysis workstation
Size/weight Main unit : 600×400×298mm 38kg
Utility box : 275×432×298mm 18kg
Environment 15 - 30oC、20 - 70%RH No condensation
Power consumption Main unit and Utility box : 100-240VAC 800VAmax, Workstation : 100-240VAC 650VAmax

*1 Under development *2 Display is not included with CQ1 system

Europe

Cenibra

Cenibra GmbH
Germany, Switzerland, Austria and Netherlands


Image Solutions, Ltd.

Image Solutions(UK), Ltd. 
UK and Ireland


Proteigene

Proteigene,
France



Accela s.r.o.,

Accela s.r.o.,,
Czech, Slovak, Hungary, Romania and Bulgaria


Kem-En-Tec Nordic A/S

Kem-En-Tec Nordic A/S,
Denmark, Sweden, Finland and Norway


OPTEC

Optec GmbH
Russia, Ukraine, Kazakhstan, Belarus, Azerbaijan, Armenia, Georgia, Uzbekistan, Turkmenistan, Kyrgyzstan, Tajikistan, and Moldova


North America

Asia

Genesci

Shanghai Genesci Medical technology Co.,Ltd.
People's Republic of China (excluding Hong Kong, Taiwan, Macao)


Tekon Biotech

Tekon Biotech
People's Republic of China, Hong Kong, Taiwan, Macao


JCBIO CO.,LTD

JCBIO CO., LTD
Republic of Korea (South Korea)

Collaborator

De Novo Software

De Novo Software
(product name: FCS Express Image Cytometry)

 

FCS Express Image Cytometry

De Novo Software has been developing flow cytometer data analysis solutions since 1998. Our flagship product, FCS Express™, is world-renowned as a robust, and easy to use flow and image cytometry data analysis application. De Novo Software offers a dedicated image analysis and reporting package for Image Cytometry to improve your workflow and results while giving you access to single cell results evening with high content screening data. FCS Express Image cytometry is directly compatible with the Yokogawa CQ1 quantitative image cytometer through the .ICE file format which enables quick import, analysis, and reporting of your results in FCS Express.

자료실

Application Note
Application Note
Overview:

Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer

Application Note
Application Note
Application Note
Overview:

The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.

Application Note
Application Note
Overview:

The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

Overview:

List of Selected Publications : CQ1

Application Note
Overview:

Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.

업종:
Yokogawa Technical Report
1.2 MB
Yokogawa Technical Report
2.2 MB
Overview:

Instrumentation devices and equipment serve for plants as the eyes, ears, nose, tongue, and skin, which are responsible for the five senses, and act as the central nervous system and brain do for human beings. The reliability and availability of such devices and equipment are the most important requirements of customers.

Overview:

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

Overview:

In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

Overview:

In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.

Overview:

In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.

Overview:

In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.

Overview:

In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.

Overview:

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

Overview:

In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.

Overview:

In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.

Overview:

In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.

Downloads

기술 및 솔루션, 요꼬가와에 대한 자세한 정보를 찾고 계십니까?

견적 및 기술문의

Top