Life Science

Your microscope can be easily upgraded to confocal microscope with Confocal Scanner Unit. The multi-beam scanning method offers not only high-speed imaging but also significantly reduced photo-toxicity and photo bleaching because of very reduced laser power of each beamlet. The CSU series have been already delivered more than 2,000 units and supported leading-edge research around the world.

News&Topics

December 029,2017

SLAS 2018

February 3-7, 2018

Courtyard by San Diego Convention Center, San Diego, CA (Booth no. 452)

We will exhibit our high-content analysis (HCA) systems at SLAS 2018. To meet the increasing demands for HCA, we have been providing LIVE CELL 3D HCA systems by our original technology. We will introduce our long-awaited NEW product, CellVoyager CV8000.

Find out more about SLAS 2018

December 029,2017 Sales news : The Discontinuation of CellVoyagerTM CV7000S High-throughput Cytological Discovery System
September 05,2017 Sales release : CellVoyagerTM CV8000 High-throughput Cytological Discovery System
  • Confocal Scanner Unit, CSU series, have been improved from the original CSU10 to the most recent CSU-X1, which are widely recognized as the de facto standard tool for live cell imaging, due to fast scanning and low photo-bleaching capability. CSU-W1 is our answer to the researchers’ request for “Wider FOV” and “Clearer Images”.

  • CellVoyager Series is a high content screening system with our original confocal scanner unit, a live cell stage incubator and a build-in liquid handler which enable long term live cell imaging and rapid kinetic analysis.

  • Confocal Quantitative Image Cytometer CQ1 offers a new approach to image quantification.

  • Label-free Morphological Analysis Software

Spinning disk confocal

microlens / fastsacnning / minimal photo bleach / high resolution

Microlens-enhanced Nipkow Disk Technology

Microlens-enhanced Nipkow Disk Technology

Fast scanning

point scanning

Point Scanning
1 line scan time=1[ms]
1000 lines/image
Scan lines=1000 [lines]
1×1000=1000 [ms]

disk scanning

Disk Scanning by CSU
Rotation Speed=10000 [rpm]=41.7[rps]
30°Rotation/image
1÷( 41.7×30/360 )= 0.5 [ms]

rotation

CSU raster scans a field of view when rotated 30 degrees:
360/30= 12scans/rotation

Scanning Speed and Resolution
Scanning Speed (fps) Resolution
CSU-W1 CSU-X1

Conventional
Line Scanning

Conventional
Point Scanning
1 2000
More than 2000×2000
1000
More than 1000×1000
512
512×512
512
512×512
30 2000
More than 2000×2000
1000
More than 1000×1000
500
512×512
64
64×64
100 2000
More than 2000×2000
1000
More than 1000×1000*1
512
512×512
impossible
500 impossible 1000
More than 1000×1000*1
100
512×100
impossible
1000 impossible 1000
More than 1000×1000*1
512
512×50
impossible
2000 impossible 1000
More than 1000×1000*1
512
512×50
impossible

※1 :In use of CSU-X1(Highend model) 

Minimal photobleaching/laser damages

Comparison between CSU and conventional LSM in 4D movies
MDCK cells stably expressing GFP-Rab25 were imaged at 2/s for 100s.
XYT-volume movies show apparent difference in the endosome fluorescence decay between the two systems.

CSU:Minimal bleaching over 100s


Conventional LSM:
Photo bleaching of each endosome is apparent

 

The CSU system is capable of reproducing the highest SNR (signal to noise ratio) measured for an SPSC system(single-beam confocal) at approximately 1/15th of the rate of the photobleaching.
 
It has been widely recognized among CSU system users, most typically cell biology investigators that extended imaging studies using the CSU10 system are remarkably free of photobleaching. Suggestions were made that its superior performance results from the high efficiency of its CCD detector or from the low illumination dosage of a spinning disc system
 
K.W.Dunn et al.,(Department of Medicine, Division of Nephrology, Indiana University Medical Center) thoroughly investigated photon economy of CSU system in comparison with that of conventional point scanning confocal systems.
 
(Journal of Microscopy, Vol. 218, Pt 2 May 2005, pp. 148 -159ng: Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems”)
 
High-speed Imaging of endosomes in living MDCK cells.

Cells expressing GFP-Rab25, a vesicle-associated protein, imaged at 20 f/s for 30s. 3X actual speed.)


Cells incubated in TexasRed-labelled transferrin which is internalized into endosomes.
Imaged at 11 f/s for 100s, 3X actual speed. Each endosome can be imaged with high S/N.


XYT volume movie of endosomes: Vertical axis represents time.
Lack of photobleaching in each endosome over time is apparent.

They quantitatively compared optical efficiency between the CSU system against several single point scanning confocal (SPSC) microscope systems, by measuring the photon economy by the signal to noise ratio(SNR) of images returned for a given level of photobleaching.

Their conclusions are:
*At moderately high imaging rates, the CSU10 system is capable of reproducing the highest SNR measured for an SPSC system at approximately 1/15th of the rate of the photobleaching.

*At higher levels of illumination, CSU system is capable of collecting images with SNRs four (4)-fold higher than the highest observed with the SPSC systems.

*Significant fluorescence saturation was found in the SPSC system, but not in the CSU10 system.

Not only is the CSU10 capable of collecting images much more rapidly than SPSC systems, it does so with much higher efficiency. The performance advantage of the CSU10 system derives not only from its more efficient collection, but also from more efficient excitation made possible by an illumination system that maximizes the population of fluorophores in the ground state, effectively optimizing the amount of fluorescence stimulated from a given number of fluorophore molecules.

Together, these characteristics support extended 4D imaging of living cells at rates sufficient to capture the 3D motion of intracellular vesicles moving up to several micrometers per second. .( K.W.Dunn et al. 2005) : Copyright 2005 The Royal Microscopical Society

High resolution

Multi-pinhole scanning with the CSU gives excellent lateral and axial resolution at high speed!

resolution

Lateral resolution

Comparison of single-shot (raw) images between CSU/confocal image and epifluorescence image (HeLa Cell expressing YFP, taken with Hamamatsu Orca 1344X1024,33msec exposure:
Image taken by Dr. T.Nagai, Hokkaido Univ.(in Japanese)

 

csuimage

Single Exposure Image (CSU)

pointscanimage

Single Exposure Image (Epifluorescence)

Enter the new world of High Content Screening with the highest-resolution imaging!

CellVoyager™ is a high-throughput cytological discovery system capable of high-speed and high-resolution imaging and analysis of biological reactions in live cells; an unsurpassed tool for effective drug development, compound evaluation,cytological functional studies, and more.

High Content Analysis

HCA is an automated research method that uses image analysis to closely examine target cells in chronological order based on single or multiple parameters. Its wide potential for investigating complex life events and phenotypes makes HCA a most promising tool for next generation drug discovery and pharmaceutical research.

  HTS Flow cytometry Microscopy
Strength High-speed handling of a large number of samples Acquires numeric data on individual cells Detailed observation of cellular events
Weakness Calculates only averages for wells Adherent cells must be removed Slow

HCA system Combines the strengths of all these conventional methods!↓

HCA system

Strength

  • Very rapidly analyzes a large number of samples
  • Acquires numeric data on individual cells
  • Detailed observation of cellular events
  • dherent cells can be observed as is
  • Temporal analysis of live cells
  • Automated high-speed imaging and analysis
December 029,2017

SLAS 2018

February 3-7, 2018

Courtyard by San Diego Convention Center, San Diego, CA (Booth no. 452)

We will exhibit our high-content analysis (HCA) systems at SLAS 2018. To meet the increasing demands for HCA, we have been providing LIVE CELL 3D HCA systems by our original technology. We will introduce our long-awaited NEW product, CellVoyager CV8000.

Find out more about SLAS 2018

December 029,2017 Sales news : The Discontinuation of CellVoyagerTM CV7000S High-throughput Cytological Discovery System
September 05,2017 Sales release : CellVoyagerTMCV8000 High-throughput Cytological Discovery System
January 19,2017

SLAS High-Content Screening Conference 2017

Tuesday, September 19, 2017 to Wednesday, September 20, 2017

Courtyard by Marriott Madrid Princesa, Calle de la Princesa 40 Madrid 28008 Spain (Booth no. 4)

We will exhibit our high-content analysis (HCA) systems at SLAS High-Content Screening Conference 2017. To meet the increasing demands for HCA, we have been providing LIVE CELL 3D HCA systems by our original technology. We will introduce our long-awaited NEW product, CellVoyager CV8000.

Find out more about SLAS High-Content Screening Conference 2017

January 19,2017

SLAS2017

Saturday, February 4, 2017 to Wednesday, February 8, 2017 (Exhibition is from Monday, February 6)

Walter E. Washington Convention Center 801 Mt. Vernon Place NW, Washington, DC USA (Booth No. 1504)

We will exhibit our High Content Analysis (HCA) systems at SLAS2017.
To meet the increasing demands for HCA, we have been providing LIVE CELL 3D HCA systems by our original technology. We will introduce our HCA systems, CellVoyager CV7000S and CQ1, and their applications.

Find out more about SLAS2017

April 04,2016

Poster presentation in 3D Cell Culture 2016, 19-21 April 2016, Konzerthaus Freiburg/Germany

Yokogawa Electric Corporation will present data obtained by our confocal image cytometer CQ1 in “3D Cell Culture 2016: How close to ‘in vivo’ can we get? Models, Application & Translation”. The poster will show the results of 3D live cell imaging and analysis of the migration and the network formation of HUVEC cells in a multilayered cell sheet. The results demonstrate that CQ1 is an excellent research tool in the field such as regenerative medicine and drug discovery screening.

*Data were provided from BioProcess Systems Engineering Lab., Dept.Biotech., Grad. Sch. Eng., Osaka University.

Poster presentation in 3D Cell Culture 2016

February 10,2016 Yokogawa Concludes Distribution Agreement with Optec, LLC for Sale of Confocal Quantitative Image Cytometer CQ1 at the markets of OPTEC activity
October 01,2015 Sales release : Label-free Morphological Analysis Software CellActivision

 

Overview:

Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression

Overview:

Discovering the Basic Principles of Life through the Live Imaging of C. elegans

Overview:

Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.

Overview:

Use of the spinning disk confocal at the Harvard Medical School microscopy core.

Overview:

New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!

Overview:

Faster, Deeper, and Clearer -in vivo molecular imaging technology-

Overview:

On-site manipulation of protein activities: Understanding intricate cell signaling pathways.

Overview:

Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.

Overview:

Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.

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