Minimally Invasive Intracellular Nano-Injector

Minimally Invasive Intracellular Nano-Injector

This system component automates the penetration and injection of single cells using a nanopipette.
It‘s low invasiveness enables manipulation of live single cells.
The system integrates with multiple manufacturers’ inverted microscopes.*1


Minimally Invasive Single Cell Analysis



  • Low Invasiveness              Adopted glass pipette with tip size of under 100 nm
  • Automated Penetration  Automated cell surface detection and penetration (Z direction movement)
  • High Success Rate             Approx. 95% success rate of injection*2
  • Singe-Cell Targeting         Enabled injection of selected cells under microscope observation
  • Rapid Injection                  Capable of injecting one cell every 10 seconds

*1 Microscope sold separately.
*2 Experiment by Yokogawa.

Function to aspirate intracellular substances is under development.


Accurate Positioning

Long stroke movements and accurate positioning enabled by combining stepping motors and piezo electric actuator.

Accurate Positioning


Accurate Positioning

Automatic cell detection and penetration with ion current measurement.

Automatic cell detection and piercing with ion current measurement


Injection Method

Utilizes electro-osmotic flow in the tip of the nanopipette to create a pump effect. The amount of injection is controlled by the duration of the voltage pulse application.


Automated Injection Process

Automatically perform approach, surface detection, injection and  retraction of the tip of the nanopipette.



Application Example

  • Direct injection of substances such as vector and genome editing tools (CRISPR/Cas9) into the nucleus
  • Efficacy/toxicity evaluation of drug candidate molecules
  • Other physical injection of reagents and proteins


Fast Injection with High Success Rate

By automating the steps to penetrate the target cell, an injection speed of approximately 10 seconds has been achieved.
Fluorescence was observed in 208 out of 220 (94.6%) HeLa cells where the fluorescent protein was injected (experiment by Yokogawa)

Below: RFP was injected into the HeLa cells, and sequentially observed with fluorescence.
HeLa cells


Low-Invasive Injection

The extremely small tip diameter of the nanopipette minimizes damage to the target cell.

Below: RFP was injected into the HeLa cells, and sequentially observed with fluorescence.
Low-Invasive Injection


Item   Specification
Basic function Injection By electroosmotic flow at tip of nanopipette
3-axis manipulator Coarse movement(Stepping Motor) Stroke:50mm/axis(setting resolution: 0.625μm)
Fine movement (Piezoelectric Element) Stroke:100μm/axis(setting resolution: 10nm)
Electronics head Voltage generation range -10V~+10V(setting resolution:10mV)
Current measurement range -100nA~+100nA
External dimensions and weight Main Controller 260(W) x 99(H) x 280(D) mm, approx. 2.5Kg
Piezoelectric Element Controller 236(W) x 88(H) x 273(D) mm, approx. 4.6kg
3-axis Manipulator 280(W) x 220(H) x 190(D) mm, approx. 2.5kg
Electronics Head 35(W) x 27(H) x 95(D) mm, approx. 0.2kg
Joystick 100(W) x 135(H) x 135(D) mm, approx. 0.5kg
Power consumption Main Controller + Piezoelectric Controller Max 100W
Ambient conditions for operation 15~35℃、20~70%RH without condensation

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