CellVoyager High-Content Analysis System CQ3000 can acquire high-resolution 3D images at high speed while culturing cells.
CQ3000 can be combined with options according to the intended use to provide a system depending on your objectives.
CQ3000 System Highlights
| Excitation laser wavelength | 405nm、488nm、561nm、640nm |
| Objective lens | 2x、4x 、10x、20x 、40x、60x (Dry 、Water immersion、Long working distance) |
| Camera | High-sensitivity sCMOS camera (up to 2 units) |
| Autofocus | Laser autofocus |
| Observation method | Confocal image, Wide-field image*1 |
| Software (Option) | High Content Analysis Software CellPathfinder |
*1 Can be acquired when Uniformizer option is selected.
Introduction
In the drug discovery market, the demand for high-content analysis systems for drug efficacy evaluation screening is ever increasing year by year.
The norm has shifted from 2D to 3D and live cell evaluation.
In addition, the construction of more complex evaluation systems are sought after, including experimental systems in environments closer to the human body such as organoids that reproduce parts of various organs or organs-on-chips, as well as Cell Painting, which morphologically profiles cellular components and organelles by multicolor imaging using multiple fluorescent dyes.
Solution
The CellVoyager High-Content Analysis System CQ3000 is a benchtop HCA system capable of acquiring high resolution images at high speed.
Yokogawa‘s proprietary technology, the Confocal Scanner Unit “CSU”, and a high-precision incubator enable stable live cell observation and high-speed imaging with low photobleaching and phototoxicity.
By combining options according to your application, we can provide the best system for you. Water immersion objective lens with high NA for easy observation of deep areas, uniform illumination effective for tile imaging such as tissue sections, and Target Search for automatic imaging of samples matching the conditions, all contribute greatly not only to improving the quality of experiments but also to speeding up and automating them.
In combination with the dedicated analysis software CellPathfinder, which supports Deep Learning, the system can support complex analysis and display results in graph form.
Make way for high-performance HCA optimized for laboratories!
High NA immersion lens for high quality imaging, Uniformizer for uniform and efficient imaging of the entire FOV, and a second camera for simultaneous imaging of two channels can be selected according to the application.
High throughput
・ Fast 3D imaging with YOKOGAWA's scanning method
・ Simultaneous dual-wavelength imaging with a second camera
・ Fast 100-fps option for high-speed applications such as myocardial and calcium firing
High definition images
・ High NA water immersion lens for bright, deep imaging
・ High quantum efficiency sCMOS camera
・ The “Uniformizer”, for uniform illumination across all corners of the image
Live-cell imaging
・ Top-class incubator performance achieves stable live cell imaging
・ Incubation time of up to 7 days
・ CSU for low bleaching and low phototoxicity imaging
Automation
・ Scans and automatically captures samples that match the conditions
・ Support for various interfaces (APIs) for external collaboration
・ Output of data types for easy integration, such as OME-TIFF and Omero
Details
High throughput imaging via simple operation
Throughput comparison when using the 2nd camera option.
Detects two colors at the same time, so acquisition time is reduced.
In addition, if confocal performance is unnecessary such as during low magnification imaging, widefield imaging can further shorten the exposure time.
When it comes to Yokogawa, it’s all about live-cell imaging
CQ3000 achieves culturing and imaging while maintaining the same environment as a conventional CO2 incubator.
It’s not only environmentally friendly, but it also minimizes the impact on cells.
High-resolution images that hold tons of information
- Water immersion lens captures clear images with a high SNR.
- In addition to fine observation via high magnification, it can accurately analyze images even with FOVs smaller than before due to the high-NA lens.
Our high-NA 20x water immersion lens developed in-house allows for image resolutions higher than a 20x dry lens. You can use 40x and 60x for efficient image analysis.
Imaging example for a spheroid
20x WI lens allows for deeper imaging with high SNR.
Uniformizer
Adjusts the excitation system for even excitation across the entire field of view. It acquires images with flat luminance distribution, with no difference in brightness between the center and periphery of the image. In tiling analysis where multiple fields of view are stitched and evaluated, it can reduce the shading-derived brightness difference at the seams between the fields of view, enabling analysis with less influence from the seams.
Software
Target Search
It can scan the entire well at low magnification, detect the position of the object based on the analysis results, and acquire images at high magnification. This makes it possible to image samples where it is not possible to know where the object is located in the well, or to image and analyze only the cells that match the conditions from among a large number of cells.
- High magnification imaging only in the field where the target is detected, greatly reducing the amount of data and throughput.
- Reduce unnecessary images and tiling by imaging at the center of the target.
- Automation of the two imaging processes reduces human intervention time and human error.
Fast Time Lapse Function
Capable of capturing up to 100 images per second (100 fps) simultaneously with two wavelengths.
It makes possible to capture fast dynamics such as myocardial pulsation, calcium firing, and intra-axonal signal transduction can be captured, which have been difficult to capture until now.
iPSC-derived cardiomyocytes in culture on a gelatin fiber substrate for cell culture (Genocell® Myocardial Evaluation Plate, Japan Wool Co.) Bright field image (left), stained with calcium-sensitive fluorescent dye (right).
Calcium signal waveform fluctuating periodically in response to myocardial pulsation (upper panel). High-speed imaging at 100 frames per second allows the fast-rising portions of the waveform to be captured at a sufficient sampling frequency. High-speed imaging at 100 frames per second allows the fast-rising portions of the waveform to be captured at a sufficient sampling frequency.
CE- Bright Field
Creates two types of contrast-enhanced images from bright-field images acquired at multiple Z positions. One is an epifluorescence-like image (Fluor Type), which is useful for recognition of cell nuclei. The other is a normal DPC-like image (Phase Type), which is useful for recognizing cytoplasm.
By enhancing the contours of the brightfield image, changes in the state of the cell can be accurately identified. It is also effective when it is difficult to recognize regions in brightfield image analysis.
Analysis software : High content analysis system CellPathfinder(Option)
The intuitive, easy-to-use interface guides the user from analyzing thousands of image data in various angles to visualize the result by generating numerous graph types.
In addition, the Machine Learning and Deep Learning function increase the target recognition capability dramatically. It is also ideal for the analysis with the complexity and high-degree-of-difficulty such as analyzing the data from 3D culture and live-cell imaging.
The CellPathfinder software is a powerful tool for HCA.
Machine learning
Software learns the features of the sample objects collected by users.
3D analysis
Label-free analysis
Wide-field*1 function is a powerful tool to analyze unstained bright field samples.
*1 Can be acquired when Uniformizer option is selected.
Specifications
| Optics | Microlens enhanced dual wide Nipkow disk confocal, Transmitted illumination |
|---|---|
| Fluorescence |
Laser : Up to 4 colors Standard : 488 nm, 561 nm Option : 405 nm, 640 nm |
| Transmitted illumination | Bright-field LED light |
| Camera | High-sensitivity sCMOS Max. 2 units, simultaneous excitation of 2 wavelengths Number of effective pixels : 2000 x 2000 pixels Field of view size: 13.0 x 13.0 mm |
| Objective lens | Max. 6 lenses (Included max. 2 water immersion lenses) Dry : 2x, 4x, 10x, 20x, 40x , 60x Long working distance : 20x, 40x Water immersion: 20x, 40x, 60x |
| Water supply function for immersion lens | Automatic supply |
| Flat-top beam shaper（Option） | Uniformizer |
| Sample vessel | Microplate (6, 12, 24, 48, 96, 384, 1536 wells), glass slides*2, cover glass chamber*2, 35 mm dish*2 |
| Stage incubator | Temperature control range 35 ~ 39 °C Settable temperature resolution : 0.1 °C Time stability : ±0.2 °C Spatial stability : ±1 °C (at an ambient temperature of 21 ~ 25 °C) Humidity holding Automatic water supply function for incubator |
| Autofocus | Laser autofocus, Image-based autofocus |
| Fast time-lapse (Option) | Max.100fps, Simultaneous dual wavelength excitation imaging |
| Sample search function (Option) |
Target Search |
| Analysis software(CellPathfinder) | Granule analysis, Neurite analysis, Nuclear morphology analysis, Nuclear translocation analysis, Membrane translocation analysis, Machine learning, Label-free analysis, 3D analysis, Texture analysis, Deep Learning, etc. |
| Other features | Self-diagnosis function, CQ Analysis |
| Size・Weight | Main unit W1031 mm x D401 mm x H600 m 84 kg Main unit (with Uniformizer or 2nd camera) W1177 mm x D401 mm x H600 mm 102 kg Utility box W275 mm x D432 mm x H298 mm 17.6 kg Gas mixer W275 mm x D432 mm x H298 mm 9.3 kg Workstation W176.5 mm x D452.1 mm x H417.9 mm 21.7 kg |
| Operating environment | 15 ~ 30°C, 30 ~ 70% RH, no condensation |
| Power consumption | Main unit and Utility box : 100 - 240 VAC, 400 VA Max Gas Mixer : 100 - 240 VAC, 60 VA Max Workstation : 100 – 240 VAC 50/60 Hz 1350 WMax |
*1 Can be acquired when Uniformizer option is selected.
*2 Sample Holder option is needed.
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YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.
In this webinar, Professor Jonny Sexton discusses a pipeline, developed in the Sexton lab, for the quantitative high-throughput image-based screening of SARS-CoV-2 infection to identify potential antiviral mechanisms and allow selection of appropriate drug combinations to treat COVID-19. This webinar presents evidence that morphological profiling can robustly identify new potential therapeutics against SARS-CoV-2 infection as well as drugs that potentially worsen COVID-19 outcomes.
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Press Release Jan 31, 2024 Yokogawa Introduces CellVoyager High-Content Analysis System CQ3000
- For greater efficiency in drug discovery and regenerative medicine R&D, and the swift commercialization of new drugs -
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