Analysis Tutorial for CellPathfinder - CellCycle-

Descarcă (133.0 MB)

To use this tutorial, a password is required. Please contact us.  Contact

Cell Cycle

In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content. DNA content is measured from the fluorescence intensity of images obtained using Hoechst staining. If we make the DNA content per nucleus equal to “1” for the G1-phase, then the DNA content doubles and becomes [2] for the G2-phase, due to the occurrence of DNA synthesis in the S-phase. Using this property, cycles are classified using the aggregate amount of intranuclear fluorescence intensity following nucleus recognition.

For the analysis, the [CellCycle] protocol pre-installed in [Application] will be used to recognize the nuclei, and the cell cycles G1-phase, G2/M-phase etc. will be classified using the Gate function. Following this, the proportion of each -phase will be displayed using a pie chart.


CellPathfinder software

Visit our download page.   Download page

To use this tutorial, a password is required. Please contact us.  Contact

Produse & Soluții similare

  • CellPathfinder

    CellPathfinder is designed for our HCA systems, CQ1 and the CellVoyager series. From beginners to experts, the analysis software lets you quantify subtle physiological changes and even label-free samples with various graph options.

    Vezi mai mult
  • CV8000 High-Throughput System

    CellVoyager CV8000 is the most advanced high-content screening system. The improved built-in incubator lets you analyze extended live cell responses. With its expandability, 4 cameras, 5 lasers and an optional built-in pipettor, the system permits increasingly complex assay development and high-content screening.

    Vezi mai mult
  • Știința Vieții

    Yokogawa’s high content analysis systems and dual spinning disk confocal technologies provide high-speed and high-resolution live cell imaging, enabling leading-edge research around the world.

    Vezi mai mult