CellVoyager CV8000 High-Content Screening System

A Yokogawa proprietary multi-scan method utilizing approximately 1,000 laser beams on the observation region and tandem disks rotating at high speed. The disks comprise a pinhole array disk with approximately 20,000 pinholes arranged in an equal pitch spiral pattern, and a microlens array disk that focuses the excitation light laser into individual pinholes. Not only does this allow high speed imaging, but it also largely prevents phototoxicity and fluorescence photobleaching.

Two different types of pinhole disks (25/50μm) can be used, according to the sample. For thick samples, reducing the pinhole diameter allows for higher confocality, shaper images. For dark samples, increasing the pinhole diameter allows for brighter images.

Organoid imaging example　Upper：25μm pinhole　Lower：50μm pinhole

The optical system configuration can be selected according to the purpose. A single 96-well plate can be imaged in four colors in one minute by attaching four high- sensitivity wide -field sCMOS cameras. The system is also compatible with FRET and CellPainting assay.

Water immersion lenses excel in capturing high-resolution images of cells within a liquid.  CV8000 can be equipped with 20x, 40x or 60x water immersion objective lens. Our 40x lens is a particularly unique lens capable of highly advanced spherical aberration correction, allowing for the capture of bright high-resolution images over an entire field of view. The lens water supply is also completely automated. This equipment makes high throughput screening via water submersion lens possible.

The stage incubator features an airtight construction, managing humidity, temperature and CO₂ levels. The integrated robotic pipetter conducts the following process fully automatically: tip pickup → reagent collection from the reagent plate → reagent addition to the sample plate → tip disposal. Not only can images be rapidly obtained before and after reagent instillation, but it’s also possible to add reagents to single wells multiple times, and adjust the addition speed etc., broadening the range of dynamics observation via reagent instillation.

Cell multiplication comparison with regular CO₂ incubator after 72hr incubation（n=3）

• 96 well average: 90
• Average of outermost 36 wells: 81
• Average of 60 wells (excl. outermost): 96

The values represent the following: CV8000 Total Area after 72hrs / Total Area at 0hrs (hereinafter Total Area ratio) / CO₂ incubator Total Area ratio x 100.
(Numbers near to 100 me an that cell multiplication was approximately equal for CV8000 and CO₂ incubator.)
Cell multiplication near to that of the CO₂ incubator was verified, excluding the four corner wells.

Cell multiplication curves for each well of a 96-well plate

• Vertical axis: Total Area
• Horizontal axis: Time (0-72 hours)

Cell multiplication was low in the four corner wells; however, it continued in the other wells.

Total Area ratio after cultivation start (24, 48 and 72 hours) (n=3)

Excluding the four corner wells, even after 72 hours, there were no large differences in cell multiplication.
The low variation in cell multiplication speed across the wells can 24 hours 48 hours 72 hours be seen.