SS2000 is a fully automated system that enables live-cell sampling of intracellular organelles — such as mitochondria and lysosomes — or even entire single cells, all while imaging cultured cells with a confocal microscope. There is no need to detach the cells from the culture dish beforehand, so spatial and morphological information remains intact. Several research publications have reported the use of SS2000 for sample collection followed by various omics analyses. Please refer to the “Publication Information” for details.
Live-Cell Imaging
Confocal microscopy enables high-speed, high-resolution 3D and time-lapse imaging of live cells.
Fully Automated, Reproducible Sampling
Automated extraction of single cells and organelles with spatial precision for reliable multi-omics analysis.
Uncover Disease Mechanisms at the Single-Cell Level
Downstream proteomic and genomic analysis reveals pathways involved in cancer and neurodegenerative diseases.
Details
Live-Cell Imaging with Confocal Microscopy
SS2000 is a system built on our proprietary live-cell imaging technology. With its unique confocal microscopy, it enables high-speed, high-resolution 3D imaging and supports time-lapse imaging to capture dynamic changes in target cells.
Target cells and sampling locations can be automatically selected through imaging analysis. (Targets are automatically selected based on cell shape, nucleus size, organelle density, etc.)
Subcellular Sampling
Sampling of target cells or intracellular components can be performed while observing them under an incubator environment. In addition, videos during sampling and images before and after sampling can be recorded, allowing users to correlate imaging data with analytical results of the collected samples.
Sampling of stress granules (FUS protein–GFP tag) in NIH/3T3 cells
Publication Information
Featuring studies that utilize our products.
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Title |
URL |
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|---|---|---|
| １ | "Single-Cell Lipidomics by LC-MS Interlaboratory Study Reveals the Impact of X-ray Irradiation on a Pancreatic Cancer Cell Line and Its Bystanders" Kyle D. G. Saunders et al., Anal Chem. 2025 Jul 1;97(25):13532-13541. |
https://pubs.acs.org/doi/10.1021/acs.analchem.5c02010 |
|
２ |
"Mass spectrometric analysis of the amiodarone-induced alteration of phosphatidylcholines in living single cells" Aogu Furusho et al., J Pharm Biomed Anal. 2025 Jun 28:265:117045. |
https://www.sciencedirect.com/science/article/abs/pii/S0731708525003863 |
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３ |
"A subcellular sampling instrument allows spatial resolution of amyloid deposit-derived organelle-specific effects in microglia" Robert Subirana Slotos et al., Commun Biol. 2025 Jan 3;8(1):3. |
https://www.nature.com/articles/s42003-024-07405-w |
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４ |
"Single-Cell Untargeted Lipidomics Using Liquid Chromatography and Data-Dependent Acquisition after Live Cell Selection" Johanna von Gerichten et al., Anal Chem. 2024 May 7;96(18):6922-6929. |
https://pubs.acs.org/doi/10.1021/acs.analchem.3c05677 |
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５ |
"Advancing single cell technology: iSCseq drives living subcellular transcriptomic profiling in osteoimmune diversity" Hiroyuki Okada et al., bioRxiv. 2024 Mar |
https://www.biorxiv.org/content/10.1101/2022.09.05.506360v4 |
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６ |
"Analysis of Live Single Cells by Confocal Microscopy and High-Resolution Mass Spectrometry to Study Drug Uptake, Metabolism, and Drug-Induced Phospholipidosis" Liliana Pedro et al., Anal Chem. 2020 Dec 15;92(24):16005-16015. |
https://pubs.acs.org/doi/10.1021/acs.analchem.0c03534 |
Product Schematic and Post-analysis Image
Product Specifications
| Automatic sampling functions | Tip diameter | 3μm, 5μm, 8μm, 10μm |
|---|---|---|
| Incubator loader environment | 37℃, 5％CO2, humidified | |
| Collection loader environment | 37℃, 5％CO2, humidified (for culture) / 4℃ (for cooling) | |
| Collection loader compatible vessels | 96-well PCR plate (0.1mL, 0.2mL) Multiwell culture plate (96well) | |
| Postioning precision of sampling | XYZ axial designated resolution : 0.1μm | |
| Imaging functions | Confocal scanning method | Microlens enhanced dual wide Nipkow disk confocal |
| Incubator loader compatible vessels | When sampling cell : φ35mm dishes *1 Microplate (6well, 24well, 96well) |
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| When observing cell : φ35mm dishes *1 Microplate (6well, 12well, 24well, 48well, 96well, 384well, 1536well) Slideglass *2 |
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| Excitation laser wavelength | 405, 488, 561, 640nm (Uniformizer installed) | |
| Emission filter | Filter size : φ25nm, Maximum slot number : 10 (Electric switching), Adjacent switching speed : 100msec | |
| Transmission illumination | Bright-field, LED source | |
| Objective lens | Dry lens : 4x, 10x, 20x, 40x Long-working distance lens : 20x, 40x Note that only the 40x dry lens can be used for cell sampling. |
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| Z focus | Electric Z motor, designated resolution : 0.1μm | |
| Electric stage | XYZ axial designated resolution : 0.1μm | |
| Autofocus | Laser autofocus | |
| Camera | sCMOS camera 2,000 x 2,000pixel Pixel size : 6.5 x 6.5μm |
*1 A sample holder is required, and with it, up to 3 samples can be installed.
*2 A sample holder is required, and with it, up to 4 samples can be installed.
Yokogawa Life Science Official Social Media Accounts
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Please follow us.
| @Yokogawa_LS | |
| Yokogawa Life Science | |
| Yokogawa Life Science | |
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News
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News Brief Apr 26, 2024 Yokogawa Helps to Revolutionize the Field of Single-Cell Lipidomics
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Press Release | Solutions & Products Dec 1, 2021 Yokogawa Develops Single Cellome System SS2000 for Subcellular Sampling
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