CSU-W1 Confocal Scanner Unit

Widest field of view in the industry providing 4X wider FOV than conventional scanners.

The CSU-W1 Confocal Scanner Unit is our widest field of view confocal, providing the clearest image resolution of our imaging systems. The system features switching mechanisms to enable fully automated experiments and a newly designed disk unit to improve image clarity of thick samples.

  • Wide and clear
  • Near Infrared (NIR) Port: Up to 785nm
  • 3 configurations: 1-camera model, 2-camera model, split-view model
  • New bright field path (Standard)
  • Selectable pinhole size:  25 pinhole disk, 50 pinhole disk or double disk
  • External light path
  • 10-position filter wheel (1 Camera model, 2 Camera model)
  • Fully automate experiments with the motorized switching mechanism

Wide

Widest FOV confocal! Provides 4 times wider FOV than the conventional model.

Widest FOV confocal


Clear

Newly designed disk unit offers much improved image quality. Due to significantly reduced pinhole crosstalk, CSU-W1 enables clear observation much deeper into thick samples.

Conventional model

XY MIP

XZ Slice

XY MIP:Conventional XZ Slice:Conventional

CSU-W1

XY MIP

XZ Slice

XY MIP:CSU-w1 XZ Slice:CSU-w1

Mouse ES cell colony
Fluorescent probe:  H2B-EGFP(Excitation:488nm) mCherry-MBD-NLS(Excitation: 561nm)
Objective lens:  60x silicone
Z-sections/stack:  100um(0.4m/251slices)
By courtesy of Jun Ueda, Ph.D. and Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University

Thin sample:Conventional,Thick sample:Conventional,Thick sample:CSU-W1

Flexible

Flexibly selectable functions to meet versatile applications.

High confocality pinhole (Optional Component)

In addition to our conventional 50um pinhole size, 25m pinhole size with higher confocality is available.
You can select either one or the both pinhole size, with easy-to-use motorized disk exchange mechanism.

Disk unit

New bright field through path (Standard)

New mechanism to move the disks out of the light path allows much easier projection of confocal and non-confocal images such as phase contrast.

Simultaneous dual color imaging mechanisms
(T2 and T3 Models)

CSU-W1 offers single camera split-view model, in addition to the dual camera model which are much improved from those for the CSU-X1. Thanks to the wide FOV, even the split-view offers 2 times wider image area than with older model. By using various dichroic mirrors, it is possible to select various dye-combinations for dual-color imaging*1 with both the two camera model and split-view model.

CSU-W1 offers selection from a total of three basic configurations, two pinhole sizes, options for near infrared observation and an external light path which is useful for versatile applications such as photo bleaching, while bright field light path is now a standard feature. All switching mechanisms in the CSU-W1 are fully motorized and thus ready for automated experiments.

Basic Configurations

CSU-W1 provides a total of three basic configurations for multi- color imaging; 1) Sequential imaging with one camera and a filter wheel, 2) Simultaneous two-color imaging with two cameras, and 3) Split-view two color imaging with one camera shared by 2 optical paths. All features are upgradable after installation.

Basic Configurations

Filter

Filter

Option

SoRa disk

Optical resolution has been improved by approximately 1.4x using a super-resolution technique based on spinning-disk confocal technology. Furthermore, a final resolution approximately twice that of the optical limit is realized through deconvolution.
Upgrading from the CSU-W1:CSU-W1 SoRa

Near Infrared (NIR) Port

NIR port provides up to 785nm excitation capability to allow less-invasive deep imaging. The NIR laser is introduced via a dedicated optical fiber in the same way as visible lasers. It is possible to combine NIR and visible lasers within the CSU-W1 unit to allow simultaneous excitation.

External light path

External light path provides the direct path bypassing the disk s to microscope. Versatile applications such as photo activation are available by introducing an external light scanner through this port.

Lens switcher

Newly designed motorized lens switcher between 2 relay lenses i s useful for fitting CSU-W1 image size with various camera types, and also for easy magnification change without exchanging objective lenses.

Variable aperture

Variable aperture to change laser illumination area, and thus the imaging area by the CSU-W1, is useful to minimize laser damages in the specimen.

Selectable option

Option 1 Camera model 2 Camera model Split-view model
NIR port ×
External light path ×
Variable aperture × N/A
Camera port lens Selectable from
0.83x, 1x
Selectable from
0.83x, 1x
(1 Camera)
Selectable from
0.83x, 1x
(2 Camera)
Selectable from
0.83x, 1x
Additional lens
to lens switcher
Selectable from 0.83x, 1x, 2x N/A

2 Camera model, 1 Camera model

2 Camera model, 1 Camera model

 

Split-view model

Split-view model

*1  2 Camera model  *2  2 Camera model and Split-view model
*3  1 Camera model and 2 Camera model  *4 Under development

External Dimensions

External dimensions

Microscope-setup
 

Zeiss Axio Observer
Zeiss Axio Observer

Nikon ECLIPSE Ti
Nikon ECLIPSE Ti

Olympus IX83
Olympus IX83

Leica DMi8
Leica DMi8

General Specifications
Model 1 camera model (T1) 2 camera model (T2) Split-view model(T3)
Confocal scanning method Microlens-enhanced Nipkow disk scanning
Spinning speed 1,500rpm - 4,000rpm Max200fps
External synchronization Scan-speed synchronization through pulse signals Input/output : TTL level 300Hz up to 800Hz
Disk unit Selectable up to 2 disks from 50um
(for high magnification)
and 25um(for low magnification)
Motorized switching
Bright field Motorized exchange between confocal and brightfield
Effective FOV 17×16mm
Excitation wavelength 405nm-785nm
Laser introduction Yokogawa's standard fiber*1 VIS Laser port (405-647nm)
【Option】NIR Laser port (685-785nm)
Observation wavelength 420nm-850nm
Dichroic mirror switching Motorized 3CH (Dichroic mirror block can be exchanged)
Emission filter wheel 10-position filter wheel
Filter sizeφ25mm
Switching speed*2 : 100msec max.(Standard mode) 40msec max.(High speed mode)
6-position filter wheel
Filter sizeφ25mm
Switching speed*2 : 100msec max.
External control RS-232C (CSU-X1 command upper compatible)
Microscope mount Yokogawa original
Camera adaptor C mount 1x (Variable magnification: 0.83x )
Light introduction port 【Option】Photo breach etc
Operating environment 15-30oC、20-75%RH No condensation
Power consumption Input : 100-240 VAC ±10% 50-60Hz 250VAmax
External dimension Main unit 327.1(W)x
251.5(D)x
475.1(H)mm
471.6(W)x
251.5(D)x
475.1(H)mm
420.8(W)x
251.5(D)x
373.6(H)mm
Power unit 225.4(W)x 151.9 (D)x 378.3(H)mm
Weight Main unit 17kg 20.5kg 18kg
Power unit 4.5kg
Attachable microscope Olympus IX series, Nikon ECLIPSE Ti , Zeiss Axio Observer, Leica DMI series*3

*1 Each CSU-W1 head is optimized with its fiber at factory. Please inquire about fiber exchange if necessary.
*2 Adjacent position.
*3 Some microscopes/options could limit the FOV of CSU-W1 or connection with CSU-W1, please inquire.

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Application Note
Prehľad:

Wide and Clear
Confocal Scanner Unit

Prehľad:

List of Selected Publications : CSU-W1

Prehľad:

To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

Yokogawa Technical Report
The World as Seen from Cells
(rd-te-r06002-001)
2.2 MB

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