CellVoyager CQ1 enables 3D imaging and quantification of live cell clusters, such as spheroids within a 3D culture vessel, as they are, keeping the cells intact. CellVoyager CQ1 exports feature data in general formats which are readable by various third-party software for advanced data analysis. It is possible to construct a fully customized CellVoyager CQ1-based system by integrating with external systems, via robot for culture dish handling.
Enables measurement of spheroids, colonies, and tissue sections
- No need to remove cells from the culture dish, in contrast to traditional flow cytometry
- Nipkow spinning disk confocal technology allows high-speed yet gentle 3D image acquisition
- Rich feature extraction to facilitate sophisticated cellular image analysis
- Wide field of view and tiling capability enables easy imaging of large specimen
Enables analysis of time-lapse and live-cell
- High precision stage incubator and low phototoxicity of our confocal technology enable time-lapse and live-cell analysis
- Max. 20 fps option for fast time lapse
High-quality image and similar operability to a traditional flow cytometer
- Feature data and statistical graphs displayed in real-time with image acquisition
- Usable high-quality image as confocal microscope image.
- Easy to trace back to the original image from a graph spot, and make repetitive measurements
Open platform
- Connectable with external systems via handling robot
- Expandable to the integrated system as image acquisition and quantification instrument
- FCS/CSV/ICE data format readable by third-party data analysis software
- A variety of cell culture and sample dishes are applicable
Details
Compact
Compact design contains fully integrated multiple functions to offer easy-to-handle confocal imaging system, without a need for complicated system integration. You only need to set a sample and run the software. User-friendly interface and versatile functions support your measurement and analysis.
Spinning Disk Confocal Scanning
A Nipkow spinning disk containing about 20,000 pinholes and a subsidiary spinning disk containing the same number of microlenses to focus excitation laser light into each corresponding pinhole are mechanically fixed on a motor, and very rapidly rotated. As a result, a high-speed raster scan of the excitation lights on the specimen can be achieved. The pinhole and microlenses are arranged on each disk in our proprietary design to optimize the raster scan. Multi-beam scanning not only increases scanning speed but also results in significantly lower photobleaching and phototoxicity because multi-beam excitation needs only a low level of laser power on the specimen to fully excite fluorescence.
Time-Lapse and Live-Cell Analysis
Support for Long-Term Live-Cell Imaging
Attachments with temperature control and humidification maintain cell viability during imaging.
The system also supports CO₂ and O₂ gas control, enabling measurements under a wide variety of environmental conditions.
Automated measurements (time-lapse imaging) can be performed at predefined intervals.
By periodically supplying water to the humidification reservoir, long-duration time-lapse imaging is possible.
When combined with a dedicated gas mixer, the system supports time-lapse observation for up to 72 hours.
The gas mixer for CellVoyagerTM CQ1
Example of HeLa Cell Proliferation Imaging
Under the conditions below, long-term time-lapse imaging over 6 days was successfully performed.
- Laboratory temperature / humidity: 22 °C / 32%
- Plate used: 96-well glass-bottom microplate #655896, Greiner (E7 well near the center)
- Humidification: Water was replenished in the humidification reservoir at the 72-hour time point
High-Speed Time-Lapse Function [Enhanced]
The high-speed time-lapse function enables image acquisition at up to 100 frames per second (100 fps).
This makes it possible to capture fast dynamic phenomena, such as the beating of iPSC-derived cardiomyocytes.
iPSC-derived cardiomyocyte beating
Top:
Example of Ca2+ signal imaging in iPSC-derived cardiomyocytes
(Objective lens: 10×, excitation wavelength: 488 nm, imaging speed: 20 fps)
Left:
Ca2+ signal waveforms in iPSC-derived cardiomyocytes
(Signals from individual cells can be measured separately.)
Note: This function is available as a paid option.
Analysis Software
- Select analysis menus from a wide range of preset options
- Display calculated numerical data in various graph formats
- Supports linking graphs to images
- Supports tile display and analysis functions
- Added area recognition method
Example of setup
| Item | Specifications |
|---|---|
| Fluorescence Excitation Light Source | 405 nm, 488 nm, 561 nm, 640 nm solid-state lasers |
| Objective lenses | Dry: 2x, 4x, 10x, 20x, 40x Long working distance: 20x, 40x Phase contrast: 10x, 20x |
| Camera | sCMOS 2000 × 2000 Pixel size: 6.5 µm |
| Autofocus | Laser-based, software-based |
| High-Speed Time-Lapse | Up to 100 fps or 20 fps (paid option) |
| Software (Option) | High-content analysis software CellPathfinder |
| Stage Heater Specifications | |
| Controllable temperature range | Room temperature +5 – +17 ℃, Max. 40 ℃ (Measured at five points: four corners and center of a 96-well plate at 37 °C setting, ambient temperature 23 °C ±2 °C) |
| Settable temperature resolution | 0.1℃ |
| Temperature stability | Within ±1 ℃ (Setting: 37 ℃, room temperature: 23 ℃ ± 2 ℃, measured point: center and 4 corners of 96 wells microplate) |
| Time to stabilize temperature | 1 hour |
| Humidity | Humidifier with a water bath unit, manual water supply (No automatic water supply) |
Remote Diagnosis Service
Equipment status can be remotely diagnosed. This service helps ensure long-term stable operation of the equipment.
Regular diagnosis can predict and detect problems early, reducing equipment downtime.
*Service available only in the USA, Japan, the UK, Switzerland, EU countries.
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Resources
Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer
The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.
Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.
List of Selected Publications : CQ1
Downloads
Software
News
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Press Release | Corporate Dec 3, 2020 Yokogawa and InSphero Enter into Partnership Agreement
- Supporting drug development research through the use of HCA and three-dimensional culture models -
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