CQ1์ 3D ๋ฐฐ์๊ธฐ ๋ด์ ํ์ ํ์์ฒด์ ๊ฐ์ ์ด์์๋ ์ ํด๋ฌ์คํฐ์ 3D ์ด๋ฏธ์ง ๋ฐ ์ ๋ํ๋ฅผ ๊ทธ๋๋ก ์ ์งํ์ฌ ์ ์ ์์์ํค์ง ์์ต๋๋ค. CQ1์ ๊ณ ๊ธ ๋ฐ์ดํฐ ๋ถ์์ ์ํด ๋ค์ํ ํ์ฌ ์ํํธ์จ์ด์์ ์ฝ์ ์ ์๋ ์ผ๋ฐ ํ์์ ๊ธฐ๋ฅ ๋ฐ์ดํฐ๋ฅผ ๋ด๋ณด๋ ๋๋ค. ๋ฐฐ์์ ์ ์กฐ์์ ์ํ ๋ก๋ด์ ํตํด ์ธ๋ถ ์์คํ *1 ๊ณผ ํตํฉํ์ฌ ์์ ํ ๋ง์ถคํ๋ CQ1 ๊ธฐ๋ฐ ์์คํ ์ ๊ตฌ์ถ ํ ์ ์์ต๋๋ค.
CQ1 ์์คํ ์ฃผ์ ํน์ง
Excitation laser wavelength | 405 nm, 488 nm, 561 nm, 640 nm |
Illumination source | Laser |
Objective lens | 2x to 60x (Dry, Phase contrast, Long working distance) |
Camera | High-sensitivity sCMOS camera |
Autofocus | Laser autofocus, Software autofocus |
Software | CellPathfinder |
ํ์ ํ์์ฒด, ์ฝ๋ก๋ ๋ฐ ์กฐ์ง ๋จ๋ฉด ์ธก์ ๊ฐ๋ฅ
- ๊ธฐ์กด์ ์ ๋ ์ธํฌ ๊ณ์ธก๋ฒ๊ณผ ๋ฌ๋ฆฌ ๋ฐฐ์ ์ ์์์ ์ธํฌ๋ฅผ ์ ๊ฑฐ ํ ํ์๊ฐ ์์ต๋๋ค.
- Nipkow ์คํผ๋ ๋์คํฌ ๊ณต์ด์ ๊ธฐ์ ๋ก ๊ณ ์์ ๋ถ๋๋ฌ์ด 3D ์ด๋ฏธ์ง ํ๋์ด ๊ฐ๋ฅํฉ๋๋ค.
- ํ๋ถํ ๊ธฐ๋ฅ ์ถ์ถ๋ก ์ ๊ตํ ์ ๋ฃฐ๋ฌ ์ด๋ฏธ์ง ๋ถ์์ด ์ฉ์ดํฉ๋๋ค.
- ๋์ ์์ผ ๋ฐ ํ์ผ๋ง ๊ธฐ๋ฅ์ ํตํด ๋ํ ์๋ฃ๋ฅผ ์ฝ๊ฒ ์ดฌ์ํ ์ ์์ต๋๋ค.
ํ์ ๋ฉ์ค ๋ฐ ๋ผ์ด๋ธ ์ ๋ถ์ ๊ฐ๋ฅ
- ๊ณ ์ ๋ฐ ์คํ ์ด์ง ์ธํ๋ฒ ์ดํฐ์ ๊ณต์ด์ ์ ๋ฎ์ ๊ด๋ ์ฑ์ผ๋ก ์๊ฐ ์ถ์ด์ ์ด์์๋ ์ ์ ๋ถ์์ด ๊ฐ๋ฅํฉ๋๋ค.
- ๊ณ ์ ๋ฉ์ค*1 ๋ฅผ ์ํ Max.20fps ์ต์
๋์ ํ์ง์ ์ด๋ฏธ์ง ๋ฐ ์ ํต์ ์ธ ํ๋ฆ ์ธํฌ์ธก์ ๊ธฐ(flow cytometer)์ ์ ์ฌํ ์กฐ์์ฑ
- ์ด๋ฏธ์ง ์์ง์ ํตํด ์ค์๊ฐ์ผ๋ก ํ์๋๋ ๊ธฐ๋ฅ ๋ฐ์ดํฐ ๋ฐ ํต๊ณ ๊ทธ๋ํ
- ๊ณต์ด์ ํ๋ฏธ๊ฒฝ ์ด๋ฏธ์ง๋ก ์ฌ์ฉ ๊ฐ๋ฅํ ๊ณ ํ์ง ์ด๋ฏธ์ง
- ๊ทธ๋ํ ์คํ์์ ์๋ ์ด๋ฏธ์ง๋ก ์ฝ๊ฒ ๋์๊ฐ๊ณ ๋ฐ๋ณต ์ธก์ ๊ฐ๋ฅ
๊ฐ๋ฐฉํ ํ๋ซํผ
- ํธ๋ค๋ง ๋ก๋ด์ ํตํด ์ธ๋ถ ์์คํ ๊ณผ ์ฐ๊ฒฐ ๊ฐ๋ฅ
- ์ด๋ฏธ์ง ์์ง ๋ฐ ์ ๋ํ ๋๊ตฌ๋ฅผ ์ด์ฉํ์ฌ ํตํฉ ์์คํ ์ผ๋ก ํ์ฅ ๊ฐ๋ฅ
- ํ์ฌ ๋ฐ์ดํฐ ๋ถ์ ์ํํธ์จ์ด์์ ์ฝ์ ์ ์๋ FCS/CSV/ICE ๋ฐ์ดํฐ ํ์
- ๋ค์ํ ์ธํฌ ๋ฐฐ์ ๋ฐ ์ํ ํ์ธ ๊ฐ๋ฅ
์ปดํฉํธํ ์ค์น ๊ณต๊ฐ, ๊ฐ๋ฒผ์ด ๋ฌด๊ฒ์ ๋ฒค์นํ ์ฅ์น, ์์ค ๋ถํ์
CQ1 | General fluorescent imaging | Flow cytometry | |
---|---|---|---|
Cell removal/suspension treatment | Not necessary | Not necessary | Necessary |
Cell image confirmation | Possible | Possible | Not possible |
Display feature data and graphs in real-time with imaging | Possible | Depends on devices | Possible |
3D data measurement | Possible | Not possible | Not possible |
Time lapse | Possible | Not possible | Not possible |
*1 ์ต์
*2 ์์ธํ ๋ด์ฉ์ CQ1 ๋ด๋น์์๊ฒ ๋ฌธ์ํ์ญ์์ค.
Details
Multiple functions fully integrated in a compact box
๋ค๋ฃจ๊ธฐ ์ฌ์ด ๊ณต์ด์ ์ด๋ฏธ์ง ์์คํ ์ ์ ๊ณตํ๊ธฐ ์ํด ๋ณต์กํ ์์คํ ํตํฉ์ ํ ํ์ ์์ด ์์ ํ ํตํฉ๋ ์ฌ๋ฌ ๊ธฐ๋ฅ๋ค์ด ์ปดํฉํธํ ๋์์ธ ์์ ๋ชจ๋ ํฌํจ๋์ด ์์ต๋๋ค; ์ฌ์ฉ์๋ ๋จ์ง ์ํ์ ์ค๋นํ๊ณ ์ํํธ์จ์ด๋ฅผ ์คํํ๊ธฐ๋ง ํ๋ฉด ๋ฉ๋๋ค. ์ฌ์ฉ์ ์นํ์ ์ธ ์ธํฐํ์ด์ค์ ๋ค์ํ ๊ธฐ๋ฅ๋ค์ด ์ธก์ ๋ฐ ๋ถ์์ ์ง์ํฉ๋๋ค.
Principles of the Microlens-enhanced Nipkow Disk Scanning Technology
์ฝ 20,000๊ฐ์ ํํ(pinholes)์ ํฌํจํ๋ Nipkow ๋ฐฉ์ฌ ๋์คํฌ์ ๊ฐ๊ฐ์ ํด๋น ํํ์ ์ฌ๊ธฐ ๋ ์ด์ ๊ด(excitation laser light)์ ์ง์ค์ํค๊ธฐ ์ํ ๋์ผํ ๊ฐฏ์์ ๋ง์ดํฌ๋ก๋ ์ฆ๋ฅผ ํฌํจํ๋ ๋ณด์กฐ ๋ฐฉ์ฌ ๋์คํฌ๊ฐ ๋ชจํฐ์ ๊ธฐ๊ณ์ ์ผ๋ก ๊ณ ์ ๋์ด ๋งค์ฐ ๋น ๋ฅด๊ฒ ํ์ ํฉ๋๋ค. ๊ฒฐ๊ณผ์ ์ผ๋ก ์๋ฃ์ ์ฌ๊ธฐ๊ด(excitation lights)์ ๋ํ ๊ณ ์ ๋์คํฐ(raster) ์ค์บ์ด ๊ฐ๋ฅํฉ๋๋ค.ํํ๊ณผ ๋ง์ดํฌ๋ก๋ ์ฆ๋ ๋์คํฐ ์ค์บ์ ์ต์ ํ๋ฅผ ์ํด ๋ ์์ ์ธ ์์ฒด ์ค๊ณ ๋์์ธ์ ๋ฐ๋ผ ๊ฐ ๋์คํฌ์ ๋ฐฐ์ด๋ฉ๋๋ค. ๋ฉํฐ๋น ์ค์บ๋์ ์ค์บ๋ ์๋๋ฅผ ์ฆ๊ฐ์ํฌ ๋ฟ๋ง ์๋๋ผ, ๋ฉํฐ๋น ์ฌ๊ธฐ(excitation)์์๋ ํ๊ด์ ์์ ํ ์ฌ๊ธฐ(excite)์ํค๊ธฐ ์ํด ํ๋ณธ์ ๋ฎ์ ์์ค์ ๋ ์ด์ ์ถ๋ ฅ๋ง ํ์ํ๊ธฐ ๋๋ฌธ์ ๊ดํ๋ฐฑ ๋ฐ ๊ด๋ ์ฑ์ด ํฌ๊ฒ ๋ฎ์์ง๋๋ค.
System integration with CQ1
Measurement Procedure
Analysis software (Option)
High content analysis system CellPathfinder*1 Click Here For More Info!
- ๋ค์ํ ์ ํ๋ฆฌ์ผ์ด์ ์ ์ํ ๋ฏธ๋ฆฌ ์ค์ ๋ ๋ถ์ ๋ฉ๋ด
- ๋ถ์ ๊ฒฐ๊ณผ๋ฅผ ๋ณด์ฌ์ฃผ๋ ์ ์ฐํ ๊ทธ๋ํ ๊ธฐ๋ฅ
- ์ฐจํธ์ ๊ฐ์ฒด ์ด๋ฏธ์ง ๊ฐ ์ง์ ์ฐ๊ฒฐ๋ ๋จธ์ ๋ฌ๋
Machine learning
Software learns the features of the sampleobjects collected by users.
3D analysis
Label-free analysis
DPC*2 function is a powerful tool to analyze unstained bright field samples.
*1 Optional software
*2 Digital phase contrast
Example of setup
Item | Specifications |
---|---|
Optics | Microlens enhanced dual wide Nipkow disk confocal, Phase contrast (Optional add-on) |
Laser/Filter | Laser : Choose 2-4 lasers from 405/488/561/640nm, 10-position Filter wheel (built-in) |
Camera | sCMOS 2560×2160pixel, 16.6×14.0mm |
Objective lens | Max.6 lenses (Dry: 2x, 4x, 10x, 20x, 40x Long working distance: 20x, 40x Phase contrast: 10x, 20x ) |
Sample vessel | Microplate (6, 24, 96, 384 well), Slide glass, Cover glass chamber*1, Dish (35, 60mm*1) |
XY stage | High-precision XY stage, designated resolution 0.1µm |
Z focus | Electric Z motor, designated resolution 0.1µm |
Autofocus | Laser autofocus, Software autofocus |
Feature data | Number of cells/cellular granules, Intensity, Volume, Surface area, Area, Perimeter, Diameter, Sphericity, Circularity, etc |
Data format | Image : 16bit TIFF file (OME-TIFF), PNG Numerical data : FCS, CSV, ICE |
Workstation | Measurement and analysis workstation |
Size/weight | Main unit : 600×400×298mm 38kg Utility box : 275×432×298mm 18kg |
Environment | 15 - 30oCใ20 - 70๏ผ RH No condensation |
Power consumption | Main unit and Utility box : 100-240VAC 800VAmax, Workstation : 100-240VAC 650VAmax |
*1 Under development *2 Display is not included with CQ1 system
์์ธํ ๋ด์ฉ์ ์๋ SNS ๊ณ์ ์์ ํ์ธํ์ญ์์ค.
Yokogawa Life Science
@Yokogawa_LS | |
Yokogawa Life Science | |
Yokogawa Life Science |
Yokogawa ๊ณต์ ์์ ๋ฏธ๋์ด ๊ณ์ ๋ชฉ๋ก
Europe
Cenibra GmbH
Germany, Switzerland, Austria and Netherlands
Image Solutions(UK), Ltd.ใ
UK and Ireland
Proteigene,
France
WITKO Sp. z o.o.,
Poland
Accela s.r.o.,,
Czech, Slovak, Hungary, Romania and Bulgaria
Kem-En-Tec Nordic A/S,
Denmark, Sweden, Finland and Norway
Optec GmbH
Russia, Ukraine, Kazakhstan, Belarus, Azerbaijan, Armenia, Georgia, Uzbekistan, Turkmenistan, Kyrgyzstan, Tajikistan, and Moldova
North America
Asia
Tekon Biotech
People's Republic of China, Hong Kong, Taiwan, Macao
JCBIO CO., LTD
Republic of Korea (South Korea)
CHAYON Laboratories
Republic of Korea (South Korea)
Collaborator
De Novo Software
(product name: FCS Express Image Cytometry)
De Novo Software has been developing flow cytometer data analysis solutions since 1998. Our flagship product, FCS Express™, is world-renowned as a robust, and easy to use flow and image cytometry data analysis application. De Novo Software offers a dedicated image analysis and reporting package for Image Cytometry to improve your workflow and results while giving you access to single cell results evening with high content screening data. FCS Express Image cytometry is directly compatible with the Yokogawa CQ1 quantitative image cytometer through the .ICE file format which enables quick import, analysis, and reporting of your results in FCS Express.
-
CellActivision
CellActivision์ Machine Learning Technology๊ณผ ๋ ์์ ์ธ ๋์งํธ ํํฐ๋ฅผ ์ฌ์ฉํ์ฌ label free ์ด๋ฏธ์ง์์ ์ง์ ์ธํฌ ๋๋ ์ฝ๋ก๋๋ฅผ ์ธ์ํ ์ ์๋๋ก ํฉ๋๋ค. CellActivision์ ์ฌ์ฉ์๊ฐ ์ค๋นํ ์ํ ๋ผ์ด๋ธ๋ฌ๋ฆฌ๋ฅผ ์ด์ฉํ์ฌ ์ฝ๊ฒ ์ธํฌ๋ฅผ ๋ถ๋ฅํ๊ณ ์ ๋ํํ ์๋ ์์ต๋๋ค.
-
CellPathfinder
CellPathfinder๋ HCA ์์คํ , CQ1 ๋ฐ CellVoyager ์๋ฆฌ์ฆ์ฉ์ผ๋ก ์ค๊ณ๋์์ต๋๋ค. ์ด๋ณด์๋ถํฐ ์ ๋ฌธ๊ฐ๊น์ง YOKOGAWA์ ๋ถ์ ์ํํธ์จ์ด๋ฅผ ์ฌ์ฉํจ์ผ๋ก์จ ๋ฏธ๋ฌํ ์๋ฆฌํ์ ๋ณํ๋ฅผ ์ ๋ํ ํ ์ ์์ผ๋ฉฐ ๋ค์ํ ๊ทธ๋ํ ์ต์ ์ ์ฌ์ฉํ์ฌ label-free ์ํ๊น์ง๋ ์ ๋ํ ํ ์ ์๊ฒ ํด ์ค๋๋ค.
์๋ฃ์ค
Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer
The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.
Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.
List of Selected Publications : CQ1
Downloads
Brochures
- CQ1 Bulletin (21.1 MB)
๋์์
YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.
๊ธฐ์ ๋ฐ ์๋ฃจ์ , ์๊ผฌ๊ฐ์์ ๋ํ ์์ธํ ์ ๋ณด๋ฅผ ์ฐพ๊ณ ๊ณ์ญ๋๊น?
๊ฒฌ์ ๋ฐ ๊ธฐ์ ๋ฌธ์