Benchtop CQ1 Confocal System

CQ1์€ 3D ๋ฐฐ์–‘๊ธฐ ๋‚ด์˜ ํšŒ์ „ ํƒ€์›์ฒด์™€ ๊ฐ™์€ ์‚ด์•„์žˆ๋Š” ์…€ ํด๋Ÿฌ์Šคํ„ฐ์˜ 3D ์ด๋ฏธ์ง• ๋ฐ ์ •๋Ÿ‰ํ™”๋ฅผ ๊ทธ๋Œ€๋กœ ์œ ์ง€ํ•˜์—ฌ ์…€์„ ์†์ƒ์‹œํ‚ค์ง€ ์•Š์Šต๋‹ˆ๋‹ค. CQ1์€ ๊ณ ๊ธ‰ ๋ฐ์ดํ„ฐ ๋ถ„์„์„ ์œ„ํ•ด ๋‹ค์–‘ํ•œ ํƒ€์‚ฌ ์†Œํ”„ํŠธ์›จ์–ด์—์„œ ์ฝ์„ ์ˆ˜ ์žˆ๋Š” ์ผ๋ฐ˜ ํ˜•์‹์˜ ๊ธฐ๋Šฅ ๋ฐ์ดํ„ฐ๋ฅผ ๋‚ด๋ณด๋ƒ…๋‹ˆ๋‹ค. ๋ฐฐ์–‘์ ‘์‹œ ์กฐ์ž‘์„ ์œ„ํ•œ ๋กœ๋ด‡์„ ํ†ตํ•ด ์™ธ๋ถ€ ์‹œ์Šคํ…œ*1 ๊ณผ ํ†ตํ•ฉํ•˜์—ฌ ์™„์ „ํžˆ ๋งž์ถคํ™”๋œ CQ1 ๊ธฐ๋ฐ˜ ์‹œ์Šคํ…œ์„ ๊ตฌ์ถ• ํ•  ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.

CQ1 ์‹œ์Šคํ…œ ์ฃผ์š” ํŠน์ง•

Excitation laser wavelength 405 nm, 488 nm, 561 nm, 640 nm
Illumination source Laser
Objective lens 2x to 60x
(Dry, Phase contrast, Long working distance)
Camera High-sensitivity sCMOS camera
Autofocus Laser autofocus, Software autofocus
Software CellPathfinder

ํšŒ์ „ ํƒ€์›์ฒด, ์ฝœ๋กœ๋‹ˆ ๋ฐ ์กฐ์ง ๋‹จ๋ฉด ์ธก์ • ๊ฐ€๋Šฅ

  • ๊ธฐ์กด์˜ ์œ ๋™ ์„ธํฌ ๊ณ„์ธก๋ฒ•๊ณผ ๋‹ฌ๋ฆฌ ๋ฐฐ์–‘ ์ ‘์‹œ์—์„œ ์„ธํฌ๋ฅผ ์ œ๊ฑฐ ํ•  ํ•„์š”๊ฐ€ ์—†์Šต๋‹ˆ๋‹ค.
  • Nipkow ์Šคํ”ผ๋‹ ๋””์Šคํฌ ๊ณต์ดˆ์  ๊ธฐ์ˆ ๋กœ ๊ณ ์†์˜ ๋ถ€๋“œ๋Ÿฌ์šด 3D ์ด๋ฏธ์ง€ ํš๋“์ด ๊ฐ€๋Šฅํ•ฉ๋‹ˆ๋‹ค.
  • ํ’๋ถ€ํ•œ ๊ธฐ๋Šฅ ์ถ”์ถœ๋กœ ์ •๊ตํ•œ ์…€๋ฃฐ๋Ÿฌ ์ด๋ฏธ์ง€ ๋ถ„์„์ด ์šฉ์ดํ•ฉ๋‹ˆ๋‹ค.
  • ๋„“์€ ์‹œ์•ผ ๋ฐ ํƒ€์ผ๋ง ๊ธฐ๋Šฅ์„ ํ†ตํ•ด ๋Œ€ํ˜• ์‹œ๋ฃŒ๋ฅผ ์‰ฝ๊ฒŒ ์ดฌ์˜ํ•  ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.

ํƒ€์ž„ ๋žฉ์Šค ๋ฐ ๋ผ์ด๋ธŒ ์…€ ๋ถ„์„ ๊ฐ€๋Šฅ

  • ๊ณ ์ •๋ฐ€ ์Šคํ…Œ์ด์ง€ ์ธํ๋ฒ ์ดํ„ฐ์™€ ๊ณต์ดˆ์ ์˜ ๋‚ฎ์€ ๊ด‘๋…์„ฑ์œผ๋กœ ์‹œ๊ฐ„ ์ถ”์ด์™€ ์‚ด์•„์žˆ๋Š” ์…€์˜ ๋ถ„์„์ด ๊ฐ€๋Šฅํ•ฉ๋‹ˆ๋‹ค.  
  • ๊ณ ์† ๋žฉ์Šค*1 ๋ฅผ ์œ„ํ•œ Max.20fps ์˜ต์…˜ 
CQ1

๋†’์€ ํ’ˆ์งˆ์˜ ์ด๋ฏธ์ง€ ๋ฐ ์ „ํ†ต์ ์ธ ํ๋ฆ„ ์„ธํฌ์ธก์ •๊ธฐ(flow cytometer)์™€ ์œ ์‚ฌํ•œ ์กฐ์ž‘์„ฑ

  • ์ด๋ฏธ์ง€ ์ˆ˜์ง‘์„ ํ†ตํ•ด ์‹ค์‹œ๊ฐ„์œผ๋กœ ํ‘œ์‹œ๋˜๋Š” ๊ธฐ๋Šฅ ๋ฐ์ดํ„ฐ ๋ฐ ํ†ต๊ณ„ ๊ทธ๋ž˜ํ”„
  • ๊ณต์ดˆ์  ํ˜„๋ฏธ๊ฒฝ ์ด๋ฏธ์ง€๋กœ ์‚ฌ์šฉ ๊ฐ€๋Šฅํ•œ ๊ณ ํ’ˆ์งˆ ์ด๋ฏธ์ง€
  • ๊ทธ๋ž˜ํ”„ ์ŠคํŒŸ์—์„œ ์›๋ž˜ ์ด๋ฏธ์ง€๋กœ ์‰ฝ๊ฒŒ ๋Œ์•„๊ฐ€๊ณ  ๋ฐ˜๋ณต ์ธก์ • ๊ฐ€๋Šฅ

๊ฐœ๋ฐฉํ˜• ํ”Œ๋žซํผ

  • ํ•ธ๋“ค๋ง ๋กœ๋ด‡์„ ํ†ตํ•ด ์™ธ๋ถ€ ์‹œ์Šคํ…œ๊ณผ ์—ฐ๊ฒฐ ๊ฐ€๋Šฅ
  • ์ด๋ฏธ์ง€ ์ˆ˜์ง‘ ๋ฐ ์ •๋Ÿ‰ํ™” ๋„๊ตฌ๋ฅผ ์ด์šฉํ•˜์—ฌ ํ†ตํ•ฉ ์‹œ์Šคํ…œ์œผ๋กœ ํ™•์žฅ ๊ฐ€๋Šฅ
  • ํƒ€์‚ฌ ๋ฐ์ดํ„ฐ ๋ถ„์„ ์†Œํ”„ํŠธ์›จ์–ด์—์„œ ์ฝ์„ ์ˆ˜ ์žˆ๋Š” FCS/CSV/ICE ๋ฐ์ดํ„ฐ ํ˜•์‹
  • ๋‹ค์–‘ํ•œ ์„ธํฌ ๋ฐฐ์–‘ ๋ฐ ์ƒ˜ํ”Œ ํ™•์ธ ๊ฐ€๋Šฅ

์ปดํŒฉํŠธํ•œ ์„ค์น˜ ๊ณต๊ฐ„, ๊ฐ€๋ฒผ์šด ๋ฌด๊ฒŒ์˜ ๋ฒค์น˜ํƒ‘ ์žฅ์น˜, ์•”์‹ค ๋ถˆํ•„์š”

  CQ1 General fluorescent imaging Flow cytometry
Cell removal/suspension treatment Not necessary Not necessary Necessary
Cell image confirmation Possible Possible Not possible
Display feature data and graphs in real-time with imaging Possible Depends on devices Possible
3D data measurement Possible Not possible Not possible
Time lapse Possible Not possible Not possible

*1 ์˜ต์…˜
*2 ์ž์„ธํ•œ ๋‚ด์šฉ์€ CQ1 ๋‹ด๋‹น์ž์—๊ฒŒ ๋ฌธ์˜ํ•˜์‹ญ์‹œ์˜ค.

Details

Multiple functions fully integrated in a compact box

๋‹ค๋ฃจ๊ธฐ ์‰ฌ์šด ๊ณต์ดˆ์  ์ด๋ฏธ์ง• ์‹œ์Šคํ…œ์„ ์ œ๊ณตํ•˜๊ธฐ ์œ„ํ•ด ๋ณต์žกํ•œ ์‹œ์Šคํ…œ ํ†ตํ•ฉ์„ ํ•  ํ•„์š” ์—†์ด ์™„์ „ํžˆ ํ†ตํ•ฉ๋œ ์—ฌ๋Ÿฌ ๊ธฐ๋Šฅ๋“ค์ด ์ปดํŒฉํŠธํ•œ ๋””์ž์ธ ์•ˆ์— ๋ชจ๋‘ ํฌํ•จ๋˜์–ด ์žˆ์Šต๋‹ˆ๋‹ค; ์‚ฌ์šฉ์ž๋Š” ๋‹จ์ง€ ์ƒ˜ํ”Œ์„ ์ค€๋น„ํ•˜๊ณ  ์†Œํ”„ํŠธ์›จ์–ด๋ฅผ ์‹คํ–‰ํ•˜๊ธฐ๋งŒ ํ•˜๋ฉด ๋ฉ๋‹ˆ๋‹ค. ์‚ฌ์šฉ์ž ์นœํ™”์ ์ธ ์ธํ„ฐํŽ˜์ด์Šค์™€ ๋‹ค์–‘ํ•œ ๊ธฐ๋Šฅ๋“ค์ด ์ธก์ • ๋ฐ ๋ถ„์„์„ ์ง€์›ํ•ฉ๋‹ˆ๋‹ค.

CQ1

Principles of the Microlens-enhanced Nipkow Disk Scanning Technology

์•ฝ 20,000๊ฐœ์˜ ํ•€ํ™€(pinholes)์„ ํฌํ•จํ•˜๋Š” Nipkow ๋ฐฉ์‚ฌ ๋””์Šคํฌ์™€ ๊ฐ๊ฐ์˜ ํ•ด๋‹น ํ•€ํ™€์— ์—ฌ๊ธฐ ๋ ˆ์ด์ € ๊ด‘(excitation laser light)์„ ์ง‘์ค‘์‹œํ‚ค๊ธฐ ์œ„ํ•œ ๋™์ผํ•œ ๊ฐฏ์ˆ˜์˜ ๋งˆ์ดํฌ๋กœ๋ Œ์ฆˆ๋ฅผ ํฌํ•จํ•˜๋Š” ๋ณด์กฐ ๋ฐฉ์‚ฌ ๋””์Šคํฌ๊ฐ€ ๋ชจํ„ฐ์— ๊ธฐ๊ณ„์ ์œผ๋กœ ๊ณ ์ •๋˜์–ด ๋งค์šฐ ๋น ๋ฅด๊ฒŒ ํšŒ์ „ํ•ฉ๋‹ˆ๋‹ค. ๊ฒฐ๊ณผ์ ์œผ๋กœ ์‹œ๋ฃŒ์˜ ์—ฌ๊ธฐ๊ด‘(excitation lights)์— ๋Œ€ํ•œ ๊ณ ์† ๋ž˜์Šคํ„ฐ(raster) ์Šค์บ”์ด ๊ฐ€๋Šฅํ•ฉ๋‹ˆ๋‹ค.ํ•€ํ™€๊ณผ ๋งˆ์ดํฌ๋กœ๋ Œ์ฆˆ๋Š” ๋ž˜์Šคํ„ฐ ์Šค์บ”์˜ ์ตœ์ ํ™”๋ฅผ ์œ„ํ•ด ๋…์ž์ ์ธ ์ž์ฒด ์„ค๊ณ„ ๋””์ž์ธ์— ๋”ฐ๋ผ ๊ฐ ๋””์Šคํฌ์— ๋ฐฐ์—ด๋ฉ๋‹ˆ๋‹ค. ๋ฉ€ํ‹ฐ๋น” ์Šค์บ๋‹์€ ์Šค์บ๋‹ ์†๋„๋ฅผ ์ฆ๊ฐ€์‹œํ‚ฌ ๋ฟ๋งŒ ์•„๋‹ˆ๋ผ, ๋ฉ€ํ‹ฐ๋น” ์—ฌ๊ธฐ(excitation)์—์„œ๋Š” ํ˜•๊ด‘์„ ์™„์ „ํžˆ ์—ฌ๊ธฐ(excite)์‹œํ‚ค๊ธฐ ์œ„ํ•ด ํ‘œ๋ณธ์— ๋‚ฎ์€ ์ˆ˜์ค€์˜ ๋ ˆ์ด์ € ์ถœ๋ ฅ๋งŒ ํ•„์š”ํ•˜๊ธฐ ๋•Œ๋ฌธ์— ๊ด‘ํ‘œ๋ฐฑ ๋ฐ ๊ด‘๋…์„ฑ์ด ํฌ๊ฒŒ ๋‚ฎ์•„์ง‘๋‹ˆ๋‹ค.

CQ1

System integration with CQ1

System integration with CQ1

Measurement Procedure

Analysis software (Option)

High content analysis system CellPathfinder*1        Click Here For More Info!

  • ๋‹ค์–‘ํ•œ ์• ํ”Œ๋ฆฌ์ผ€์ด์…˜์„ ์œ„ํ•œ ๋ฏธ๋ฆฌ ์„ค์ •๋œ ๋ถ„์„ ๋ฉ”๋‰ด
  • ๋ถ„์„ ๊ฒฐ๊ณผ๋ฅผ ๋ณด์—ฌ์ฃผ๋Š” ์œ ์—ฐํ•œ ๊ทธ๋ž˜ํ”„ ๊ธฐ๋Šฅ
  • ์ฐจํŠธ์™€ ๊ฐœ์ฒด ์ด๋ฏธ์ง€ ๊ฐ„ ์ง์ ‘ ์—ฐ๊ฒฐ๋œ ๋จธ์‹ ๋Ÿฌ๋‹

Machine learning


Software learns the features of the sampleobjects collected by users.

3D analysis


 

Label-free analysis
DPC*2 function is a powerful tool to analyze unstained bright field samples.

*1 Optional software
*2 Digital phase contrast

Example of setup

Example of setup

 

Item Specifications
Optics Microlens enhanced dual wide Nipkow disk confocal,
Phase contrast (Optional add-on)
Laser/Filter Laser : Choose 2-4 lasers from 405/488/561/640nm,
10-position Filter wheel (built-in)
Camera sCMOS 2560×2160pixel, 16.6×14.0mm
Objective lens Max.6 lenses
(Dry: 2x, 4x, 10x, 20x, 40x Long working distance: 20x, 40x
Phase contrast: 10x, 20x )
Sample vessel Microplate (6, 24, 96, 384 well), Slide glass,
Cover glass chamber*1, Dish (35, 60mm*1)
XY stage High-precision XY stage, designated resolution 0.1µm
Z focus Electric Z motor, designated resolution 0.1µm
Autofocus Laser autofocus, Software autofocus
Feature data Number of cells/cellular granules, Intensity, Volume, Surface area, Area, Perimeter, Diameter, Sphericity, Circularity, etc
Data format Image : 16bit TIFF file (OME-TIFF), PNG
Numerical data : FCS, CSV, ICE
Workstation Measurement and analysis workstation
Size/weight Main unit : 600×400×298mm 38kg
Utility box : 275×432×298mm 18kg
Environment 15 - 30oCใ€20 - 70๏ผ…RH No condensation
Power consumption Main unit and Utility box : 100-240VAC 800VAmax, Workstation : 100-240VAC 650VAmax

*1 Under development *2 Display is not included with CQ1 system

์ž์„ธํ•œ ๋‚ด์šฉ์€ ์•„๋ž˜ SNS ๊ณ„์ •์—์„œ ํ™•์ธํ•˜์‹ญ์‹œ์˜ค.

 

Yokogawa Life Science

•Twitter @Yokogawa_LS
•Facebook Yokogawa Life Science
•LinkedIn Yokogawa Life Science

 

Yokogawa ๊ณต์‹ ์†Œ์…œ ๋ฏธ๋””์–ด ๊ณ„์ • ๋ชฉ๋ก

Social Media Account List

Europe

Cenibra

Cenibra GmbH
Germany, Switzerland, Austria and Netherlands


Image Solutions, Ltd.

Image Solutions(UK), Ltd.ใ€€
UK and Ireland


Proteigene

Proteigene,
France



Accela s.r.o.,

Accela s.r.o.,,
Czech, Slovak, Hungary, Romania and Bulgaria


Kem-En-Tec Nordic A/S

Kem-En-Tec Nordic A/S,
Denmark, Sweden, Finland and Norway


OPTEC

Optec GmbH
Russia, Ukraine, Kazakhstan, Belarus, Azerbaijan, Armenia, Georgia, Uzbekistan, Turkmenistan, Kyrgyzstan, Tajikistan, and Moldova


 

North America

 

Asia

Tekon Biotech

Tekon Biotech
People's Republic of China, Hong Kong, Taiwan, Macao


JCBIO CO.,LTD

JCBIO CO., LTD
Republic of Korea (South Korea)


CHAYON Laboratories
Republic of Korea (South Korea)

 

Collaborator

De Novo Software

De Novo Software
(product name: FCS Express Image Cytometry)

 

FCS Express Image Cytometry

De Novo Software has been developing flow cytometer data analysis solutions since 1998. Our flagship product, FCS Express™, is world-renowned as a robust, and easy to use flow and image cytometry data analysis application. De Novo Software offers a dedicated image analysis and reporting package for Image Cytometry to improve your workflow and results while giving you access to single cell results evening with high content screening data. FCS Express Image cytometry is directly compatible with the Yokogawa CQ1 quantitative image cytometer through the .ICE file format which enables quick import, analysis, and reporting of your results in FCS Express.

  • CellActivision

    CellActivision์€ Machine Learning Technology๊ณผ ๋…์ž์ ์ธ ๋””์ง€ํ„ธ ํ•„ํ„ฐ๋ฅผ ์‚ฌ์šฉํ•˜์—ฌ label free ์ด๋ฏธ์ง€์—์„œ ์ง์ ‘ ์„ธํฌ ๋˜๋Š” ์ฝœ๋กœ๋‹ˆ๋ฅผ ์ธ์‹ํ•  ์ˆ˜ ์žˆ๋„๋ก ํ•ฉ๋‹ˆ๋‹ค. CellActivision์€ ์‚ฌ์šฉ์ž๊ฐ€ ์ค€๋น„ํ•œ ์ƒ˜ํ”Œ ๋ผ์ด๋ธŒ๋Ÿฌ๋ฆฌ๋ฅผ ์ด์šฉํ•˜์—ฌ ์‰ฝ๊ฒŒ ์„ธํฌ๋ฅผ ๋ถ„๋ฅ˜ํ•˜๊ณ  ์ •๋Ÿ‰ํ™”ํ•  ์ˆ˜๋„ ์žˆ์Šต๋‹ˆ๋‹ค.

    See More
  • CellPathfinder

    CellPathfinder๋Š” HCA ์‹œ์Šคํ…œ, CQ1 ๋ฐ CellVoyager ์‹œ๋ฆฌ์ฆˆ์šฉ์œผ๋กœ ์„ค๊ณ„๋˜์—ˆ์Šต๋‹ˆ๋‹ค. ์ดˆ๋ณด์ž๋ถ€ํ„ฐ ์ „๋ฌธ๊ฐ€๊นŒ์ง€ YOKOGAWA์˜ ๋ถ„์„ ์†Œํ”„ํŠธ์›จ์–ด๋ฅผ ์‚ฌ์šฉํ•จ์œผ๋กœ์จ ๋ฏธ๋ฌ˜ํ•œ ์ƒ๋ฆฌํ•™์  ๋ณ€ํ™”๋ฅผ ์ •๋Ÿ‰ํ™” ํ•  ์ˆ˜ ์žˆ์œผ๋ฉฐ ๋‹ค์–‘ํ•œ ๊ทธ๋ž˜ํ”„ ์˜ต์…˜์„ ์‚ฌ์šฉํ•˜์—ฌ label-free ์ƒ˜ํ”Œ๊นŒ์ง€๋„ ์ •๋Ÿ‰ํ™” ํ•  ์ˆ˜ ์žˆ๊ฒŒ ํ•ด ์ค๋‹ˆ๋‹ค.

    See More

์ž๋ฃŒ์‹ค

Application Note
Application Note
Overview:

Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer

Application Note
Application Note
Overview:

The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

Application Note
Overview:

Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.

์—…์ข…:
Application Note
Yokogawa Technical Report
1.0 MB
Overview:

List of Selected Publications : CQ1

Downloads

Brochures

๋™์˜์ƒ

Overview:

YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.

๊ธฐ์ˆ  ๋ฐ ์†”๋ฃจ์…˜, ์š”๊ผฌ๊ฐ€์™€์— ๋Œ€ํ•œ ์ž์„ธํ•œ ์ •๋ณด๋ฅผ ์ฐพ๊ณ  ๊ณ„์‹ญ๋‹ˆ๊นŒ?

๊ฒฌ์  ๋ฐ ๊ธฐ์ˆ ๋ฌธ์˜

Top