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Our microscopy and life sciences solutions are designed to support applications from basic research to drug discovery to pre-clinical trials.

Yokogawa’s high content analysis systems and dual spinning disk confocal technologies are used in regenerative medicine, drug discovery, and precision medicine, providing high-speed and high-resolution live cell imaging.

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Principles of Spinning disk confocal

The most common conventional confocal microscopes use a single laser beam to scan a specimen, while the CSU scans the field of view with approximately 1,000 laser beams, by using microlens-enhanced Nipkow-disk scanning: in short, CSU can scan 1,000 times faster.

By using a disk containing microlens arrays in combination with the Nipkow disk, we have succeeded in dramatically improving the light efficiency and thus successfully made real-time confocal imaging of live cells possible.

The expanded and collimated laser beam illuminates the upper disk containing about 20,000 microlenses (microlens array disk). Each microlens focuses the laser beam onto its corresponding pinhole, thus, effectively increasing laser intensity through pinholes placed in the pinhole array disk (Nipkow disk).

With the microlens, backscattering of laser light at the surface of the pinhole disk can be significantly reduced, thus, dramatically increasing the signal to noise ratio (S/N) of confocal images.

About 1,000 laser beams passing through each of the pinholes fill the aperture of the objective lens, and are then focused on the focal plane. Fluorescence generated from the specimen is captured by the objective lens and focused back onto the pinhole disk, transmitted through the same holes to eliminate out-of-focus signals, deflected by the dichroic mirror located between microlens array disk and the Nipkow disk to split fluorescence signal from reflected laser, passed through emission filter and then focused into the image plane in the eyepiece or camera.

The microlens array disk and the Nipkow disk are physically fixed to each other and are rotated to scan the entire field of view at high speeds, thus, making it possible to view confocal fluorescent images in real-time through the eyepiece of the CSU head.

As compared to conventional single point scanning, multi beam scanning by the CSU requires a significantly low level of light intensity per unit area, which results in significantly reduced photo bleaching and phototoxicity in live cells.

Spinning Disk Confocal

microlens / fastsacnning / minimal photo bleach / high resolution

Microlens-enhanced Nipkow Disk Technology

Microlens-enhanced Nipkow Disk Technology

Comparison of scanning method

point scanning

Point Scanning
1 line scan time=1[ms]
1000 lines/image
Scan lines=1000 [lines]
1×1000=1000 [ms]

disk scanning

Disk Scanning by CSU
Rotation Speed=10000 [rpm]=41.7[rps]
30°Rotation/image
1÷( 41.7×30/360 )= 0.5 [ms]

 

 

April 8,2019

FOM 2019

April 14-17, 2019
Queen Elizabeth II Center
Booth No. 42

We will exhibit Spinning disk confocal "CSU-W1 SoRa".

Link to products
Super Resolution Confocal Scanner Unit : CSU-W1 SoRa
Confocal Scanner Unit : CSU-W1

Find out more about FOM 2019

January 16,2019

SLAS 2019

February 4-6, 2019
Walter E. Washington Convention Center
Booth No. 214

We will exhibit high content analysis system "CellVoyager".

Link to products
CellVoyagerTMCV8000 High-throughput Cytological Discovery System
Confocal Quantitative Image Cytometer CQ1
High Content Analysis Software CellPathfinder

*Poster presentation is planned. Details will be posted as soon as it is decided.

Find out more about SLAS 2019

October 24,2018

ASCB/EMBO 2018

December 9-11, 2018
Booth No. 845

-Tech talks-

December 9, 3:00-4:00 pm – Theater 2, Learning Center

Super Resolution Confocal Scanner Unit CSU-W1 Sora

Presenter: Takuya Azuma: Chief designer of CSU-W1 Sora,
Yoshitaka Sekizawa: Product manager of CSU-W1 Sora

Yokogawa will introduce our brand-new product “CSU-W1 SoRa.” This is a spinning disk based super resolution confocal scanner unit. In this talk, we will introduce features and principles of this product and we will show beautiful image samples taken by “CSU-W1 SoRa”. Features of “CSU-W1 SoRa”: 1) XY resolution of approx. 120nm. XY resolution has been improved by approximately 1.4x the optical limit based on spinning-disk confocal technology. Furthermore, a final resolution approximately twice the optical limit is realized through deconvolution. 2) Ideal for super-resolution live cell imaging. Just like the CSU, high-speed real time imaging can be performed with super-resolution. In addition, live cell imaging is possible, reducing bleaching and phototoxicity. 3) The CSU is easy to use. Super-resolution images can be observed in real time without any specific preparation of sample. Deep position observation is made possible through optical sectioning based on confocal technology. 4) Upgradable from CSU-W1. If you already have CSU-W1, you can add SoRa disk.

Find out more about ASCB/EMBO 2018

September 14,2018

Sales release : High Content Analysis Software CellPathfinder

 Click Here For More Info

July 27,2018

Sales release : High-speed Super resolution Confocal Scanner CSU-W1 SoRa

 Click Here For More Info

June 11,2018

2018 SLAS Europe

March 01,2018

Sales release : High Content Data Management System CellLibrarian

 Click Here For More Info

December 29,2017

SLAS 2018

February 3-7, 2018

December 29,2017 Sales news : The Discontinuation of CellVoyagerTM CV7000S High-throughput Cytological Discovery System
September 05,2017

Sales release : CellVoyagerTMCV8000 High-throughput Cytological Discovery System

 Click Here For More Info

January 19,2017

SLAS High-Content Screening Conference 2017

Find out more about SLAS High-Content Screening Conference 2017

April 04,2016

Poster presentation in 3D Cell Culture 2016, 19-21 April 2016, Konzerthaus Freiburg/Germany

Yokogawa Electric Corporation will present data obtained by our confocal image cytometer CQ1 in “3D Cell Culture 2016: How close to ‘in vivo’ can we get? Models, Application & Translation”. The poster will show the results of 3D live cell imaging and analysis of the migration and the network formation of HUVEC cells in a multilayered cell sheet. The results demonstrate that CQ1 is an excellent research tool in the field such as regenerative medicine and drug discovery screening.

*Data were provided from BioProcess Systems Engineering Lab., Dept.Biotech., Grad. Sch. Eng., Osaka University.

Poster presentation in 3D Cell Culture 2016

February 10,2016 Yokogawa Concludes Distribution Agreement with Optec, LLC for Sale of Confocal Quantitative Image Cytometer CQ1 at the markets of OPTEC activity
October 01,2015 Sales release : Label-free Morphological Analysis Software CellActivision

 

Zdroje

Přehled:

Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression

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Faster, Deeper, and Clearer -in vivo molecular imaging technology-

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Discovering the Basic Principles of Life through the Live Imaging of C. elegans

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Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.

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New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!

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Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.

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Use of the spinning disk confocal at the Harvard Medical School microscopy core.

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Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.

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On-site manipulation of protein activities: Understanding intricate cell signaling pathways.

Aplikační listy
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A critical requirement in biopharmaceutical development is the integration and automation of process equipment and analytical instruments used in the laboratory. Bioprocess labs with multiple lab-scale bioreactors often execute cultivation experiments in parallel for research or process development purposes.

As part of a collaboration between Securecell (Zurich, SW) and Yokogawa Life Science (Tokyo, Japan), this application note demonstrates the effective use of the Lucullus® Process Information Management System (Lucullus®) to assist in the control of three Advanced Control Bioreactor Systems (BR1000) to study glucose utilization of CHO cells for optimal monoclonal antibody productivity.

Průmysly:
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Comparison between CSU and conventional LSM in 4D movies.

Aplikační listy
Aplikační listy
Aplikační listy
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CV1000 clears the hurdle in Live Cell Imaging
All-in-one Live cell imaging solution

Aplikační listy
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To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

Aplikační listy
Aplikační listy
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The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.

Aplikační listy
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Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.

Průmysly:
Aplikační listy
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This application note will introduce the features of the SU10 and provide examples demonstrating the delivery of genome editing tools (Cas9 RNP) using the technology.

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The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

Přehled:

Caustic soda is an important basic material in the chemical industry and is mainly produced by the electrolysis of soda. In the electrolysis process to make concentrated caustic soda, the DM8 Liquid Density Meter ensures high product quality through accurate measurement of liquid density.

Aplikační listy
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List of Selected Publications : CSU-W1

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List of Selected Publications : CQ1

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List of Selected Publications : CSU-X1

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List of Selected Publications : CV8000, CV7000, CV6000

Aplikační listy
Přehled:
  • Colony Formation
  • Scratch Wound
  • Cytotoxicity
  • Neurite Outgrowth
  • Co-culture Analysis
  • Cell Tracking
Průmysly:
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Faster, Brighter, and More Versatile Confocal Scanner Unit

Průmysly:
Aplikační listy
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Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System

Aplikační listy
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Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer

Aplikační listy
Přehled:

Wide and Clear
Confocal Scanner Unit

Průmysly:
Technické reporty Yokogawa
2.2 MB
Technické reporty Yokogawa
6.1 MB
Přehled:

This "Tutorial" provides overview of this software, from installation through data analysis.

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In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

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In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

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In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.

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In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

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In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.

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In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.

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In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.

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In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.

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In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.

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In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.

Downloads

Videa

Přehled produktů
Přehled:

The Yokogawa business vision states that the company endeavors to achieve Net-zero emissions, ensure the Well-being of all, and make a transition to a Circular Economy by 2050. 

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YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.

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YOKOGAWA aspires to establish Smart GMP manufacturing facilities that provide consistent quality and supply while eliminating industrial waste, enhancing productivity and always using high-quality component parts and materials.

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YOKOGAWA creates autonomous operations with high-efficiency automation and optimization that allows growth with minimal deployment of manpower.

Přehled:

In this webinar, Professor Jonny Sexton discusses a pipeline, developed in the Sexton lab, for the quantitative high-throughput image-based screening of SARS-CoV-2 infection to identify potential antiviral mechanisms and allow selection of appropriate drug combinations to treat COVID-19. This webinar presents evidence that morphological profiling can robustly identify new potential therapeutics against SARS-CoV-2 infection as well as drugs that potentially worsen COVID-19 outcomes.

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