Life Science

Early stage embryo

Our microscopy and life sciences solutions are designed to support applications from basic research to drug discovery and pre-clinical trials.

Yokogawa’s high content analysis systems and dual spinning disk confocal technologies are used in regenerative medicine, pharmaceutical research, and precision medicine to deliver rapid, high-resolution live cell imaging.

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Principles of Spinning disk confocal

The most common conventional confocal microscopes use a single laser beam to scan a specimen, while the CSU scans the field of view with approximately 1,000 laser beams, by using microlens-enhanced Nipkow-disk scanning: in short, CSU can scan 1,000 times faster.

By using a disk containing microlens arrays in combination with the Nipkow disk, we have succeeded in dramatically improving the light efficiency and thus successfully made real-time confocal imaging of live cells possible.

The expanded and collimated laser beam illuminates the upper disk containing about 20,000 microlenses (microlens array disk). Each microlens focuses the laser beam onto its corresponding pinhole, thus, effectively increasing laser intensity through pinholes placed in the pinhole array disk (Nipkow disk).

With the microlens, backscattering of laser light at the surface of the pinhole disk can be significantly reduced, thus, dramatically increasing the signal to noise ratio (S/N) of confocal images.

About 1,000 laser beams passing through each of the pinholes fill the aperture of the objective lens, and are then focused on the focal plane. Fluorescence generated from the specimen is captured by the objective lens and focused back onto the pinhole disk, transmitted through the same holes to eliminate out-of-focus signals, deflected by the dichroic mirror located between microlens array disk and the Nipkow disk to split fluorescence signal from reflected laser, passed through emission filter and then focused into the image plane in the eyepiece or camera.

The microlens array disk and the Nipkow disk are physically fixed to each other and are rotated to scan the entire field of view at high speeds, thus, making it possible to view confocal fluorescent images in real-time through the eyepiece of the CSU head.

As compared to conventional single point scanning, multi beam scanning by the CSU requires a significantly low level of light intensity per unit area, which results in significantly reduced photo bleaching and phototoxicity in live cells.

Spinning Disk Confocal

microlens / fastsacnning / minimal photo bleach / high resolution

Microlens-enhanced Nipkow Disk Technology

Microlens-enhanced Nipkow Disk Technology

Comparison of scanning method

point scanning

Point Scanning
1 line scan time=1[ms]
1000 lines/image
Scan lines=1000 [lines]
1×1000=1000 [ms]

disk scanning

Disk Scanning by CSU
Rotation Speed=10000 [rpm]=41.7[rps]
30°Rotation/image
1÷( 41.7×30/360 )= 0.5 [ms]

 

 

February 18,2022

Sales Release : Single CellomeTM System SS2000

We announce the sales release of Single Cellome System SS2000 on February 18th, 2022.

Advantages

  • Automatic sampling of specific intracellular components or whole cells
  • High resolution images and imaging analysis using confocal microscopy
  • Precise pipetting control of location works
  • Sampling with location and morphology information
  • Keeping cell activity via incubator functions

Release Date
Japan, North America, China, Europe*: February 18th, 2022
It will be successively launched in other regions.
*Some countries are not eligible.

Please contact us for the details.

Click Here For More Info

February 15,2022

Sales release : Confocal Quantitative Image Cytometer CellVoyager CQ1 new option and latest software  were released

  • High-speed time-lapse option (100 fps), an even faster model is now available (paid option).
  • The latest software (R1.07.01) is now available!
    Expected to improve environmental control for live cell imaging and increase throughput at low magnifications!

Click Here For More Info 

February 15,2022

Sales release : CellPathfinder new product and the latest software were released

  • Subscription sales launched!
    Available to select necessary items from flexible license conditions. [Function], [Period] and [Support Style] are all selectable.
  • Sales of Function Selective Products started
    Concept of [Necessary Function for When Needed]
    Available to offer Basic Pack with Options style.
  • The latest software (R3.06.01) is now available!
    By down sampling, available to create more than 20,000x20,000 pixel of tyle images, and observe overall picture for checking.
    Expected to improve analysis speed when spatial resolution is not essentially required.

Click Here For More Info 

December 1,2021

Yokogawa Develops Single Cellome System SS2000 for Intracellular Sampling
- A single-cell analysis solution that revolutionizes efficiency in drug discovery research by fully automating the collection of intracellular components and specific cells -
 
Yokogawa Electric Corporation (TOKYO: 6841) announces that it has developed the Single Cellome™ System SS2000, a single-cell analysis solution that utilizes high-definition images captured with a confocal microscope to accurately collect samples of intracellular components and specific cells.

<Cell sampling> 
・High resolution imaging using confocal microscope
・Precisely analyzing for localization of intracellular components by 3D imaging
・Accurately positioning by the X, Y, and Z directions
・Sampling that maintains the positional and morphological information of cells

<Features of the SS2000> 
・Automatic sampling of specific intracellular components or whole cells
・High resolution images and imaging analysis using confocal microscopy
・Precise pipetting control of location works
・Sampling with location and morphology information
・Keeping cell activity via incubator functions

The SS2000 will be released to the Japan, US and China market in January 2022, with release in other markets such as Europe to follow at a later date.

Press Release: Yokogawa Develops Single Cellome System SS2000 for Intracellular Sampling

November 18,2021

Yokogawa Acquires Insilico Biotechnology, Developer of Innovative Bioprocess Digital Twin Technology
Yokogawa Electric Corporation acquire Insilico Biotechnology a developer and provider of bioprocess software and services.

Press Release:Yokogawa Acquires Insilico Biotechnology, Developer of Innovative Bioprocess Digital Twin Technology

January 8,2021

Sales release : Advanced Control Bioreactor System BR1000 was released.
The Advanced Control Bioreactor System BR1000 automates lab-scale mammalian cell culture with highly accurate real-time monitoring and advanced process control.

Click Here For More Info 

August 20,2020

Discovering Potential Covid-19 Therapies using High Content Screening

Date: Wednesday August 26th 2020
Time: 12pm (EST) 9am (PST) 5pm (London)

Abstract:
In this webinar, Professor Jonny Sexton discusses a pipeline, developed in the Sexton lab, for the quantitative high-throughput image-based screening of SARS-CoV-2 infection to identify potential antiviral mechanisms and allow selection of appropriate drug combinations to treat COVID-19. This webinar presents evidence that morphological profiling can robustly identify new potential therapeutics against SARS-CoV-2 infection as well as drugs that potentially worsen COVID-19 outcomes.

Click Here for  Registration

June 5,2020

Sales release : High-throughput Cytological Discovery System CV8000 : 20x water immersion lens option was released.

 Click Here For More Info

March 18,2020

Sales release : Single-cell Analysis Solution Single Cellome Unit SU10

 Click Here For More Info

January 20,2020

Sales release : High Content Analysis Software CellPathfinder update and Deep learning option was released. 

Deep learning function succeeded in dramatically improving by recognizing patterns in images to identify what the images show.

Link to products High Content Analysis Software CellPathfinder
Find out more about Deep learning

January 15,2020

Society for Laboratory Automation and Screening (SLAS) 2020

January 25-29, 2020
Booth No. 1148

We will exhibit high content analysis system "CellVoyager CQ1".

Link to products
Confocal Quantitative Image Cytometer CQ1

-Poster-

1207-C:
A Live Cell High Content Assay for Cellular Lipid Droplets
January 28, 2:00-3:00 pm

Find out more about SLAS 2020

 

Файлы

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Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression

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Faster, Deeper, and Clearer -in vivo molecular imaging technology-

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Discovering the Basic Principles of Life through the Live Imaging of C. elegans

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Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.

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New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!

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Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.

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Use of the spinning disk confocal at the Harvard Medical School microscopy core.

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Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.

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On-site manipulation of protein activities: Understanding intricate cell signaling pathways.

Application Note
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Comparison between CSU and conventional LSM in 4D movies.

Application Note
Application Note
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CV1000 clears the hurdle in Live Cell Imaging
All-in-one Live cell imaging solution

Application Note
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To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

Application Note
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The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.

Application Note
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Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.

Отрасли:
Application Note
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The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

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Caustic soda is an important basic material in the chemical industry and is mainly produced by the electrolysis of soda. In the electrolysis process to make concentrated caustic soda, the DM8 Liquid Density Meter ensures high product quality through accurate measurement of liquid density.

Application Note
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List of Selected Publications : CSU-W1

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List of Selected Publications : CQ1

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List of Selected Publications : CSU-X1

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List of Selected Publications : CV8000, CV7000, CV6000

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  • Colony Formation
  • Scratch Wound
  • Cytotoxicity
  • Neurite Outgrowth
  • Co-culture Analysis
  • Cell Tracking
Отрасли:
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Faster, Brighter, and More Versatile Confocal Scanner Unit

Отрасли:
Application Note
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Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System

Application Note
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Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer

Application Note
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Wide and Clear
Confocal Scanner Unit

Отрасли:
Yokogawa Technical Report
2.2 MB
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This "Tutorial" provides overview of this software, from installation through data analysis.

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In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

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In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

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In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.

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In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

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In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.

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In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.

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In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.

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In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.

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In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.

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In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.

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