The Advanced Control Bioreactor System BR1000 automates lab-scale mammalian cell culture with highly accurate real-time monitoring and advanced process control.
Based on YOKOGAWA's original calibration model and instrumentation, the concentration of glucose and lactic acid in the culture medium can be measured in real-time and with high accuracy based on the absorption characteristics of near-infrared light.
Based on YOKOGAWA's original calibration model, the live cell density can be measured in real time from changes in the capacitance characteristics of the cell suspension. The unique calibration model also maintains high accuracy throughout the entire cell culture timeline, from seeding to high cell densities.
A model prediction and control algorithm processes the data obtained by the in-line sensors and outputs a highly accurate glucose consumption rate trend in real time.
An advanced control algorithm designed specifically for cell culture provides accurate and automatic control of glucose based on in-line sensor values. Peristaltic pump systems dynamically deliver sterile glucose solution as needed to maintain the glucose set-point concentration value.
The instrument provides basic control of pH, temperature, and dissolved oxygen, a near-infrared sensor for monitoring cell activity, a viable cell density sensor, feed control, and data logger, all integrated into one complete system.
The bioreactor system provides advanced process control driven by innovative hardware and software technologies to improve reproducibility and stability of cell culture conditions for better biopharmaceuticals.
Comes complete with a calibration model derivation tool that customizes and improves the accuracy of in-line sensors.
Provides the capability for real-time remote monitoring in a networked environment.
|Liquid delivery pump||Number of channels: 4/6|
|Stirrer control||Control range: 4-200rpm|
|Temperature control||Control range: 34-40℃|
|pH control||Control range: 6.5-7.5|
|Dissolved oxygen control||Control range: 1-5mg/L|
|In-line measurement of glucose concentration||Measurement range: 0-10.0g/L|
|In-line measurement of lactate concentration||Measurement range: 0-10.0g/L|
|In-line measurement of viable cell density||Measurement range: 1×105-1×108cells/mL|
|Other standard functions||Culture vessel (cell culture medium) weight monitor, Feed bottle weight monitor, Automation control disengagement button|
|User interface||Touch panel monitor|
|Log data file format||Custom file record format (GM10) (*.GSD *GEV)|
|Classification of laser product||Class 1 Laser Product (IEC 60825-1：2007/2014)|
|Operating environment||20 to 26℃, 20 to 80%RH without condensation, Altitude ≦ 1000m|
|Power supply||Single phase 100/115/200/230VAC, 50/60Hz, Rated power: 500W|
Yokogawa's in-line sensors and advanced control algorithms will stabilize and improve the efficiency of the cell culture processes for biopharmaceutical production.
This paper describes how the Yokogawa BR1000 bioreactor can be used to automate through real-time sensing and improve productivity through accurate and efficient glucose control.
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