A portfolio of flexible life science products and solutions for the regenerative medicine, pharmaceutical research, and precision medicine industries.
Yokogawa’s high content analysis systems and dual spinning disk technologies are known for higher product quality, more individualized systems tuning, and better process control. From design to implementation and startup to continuous optimization, Yokogawa has the experience and technology to solve your greatest challenges.
- Yokogawa’s Spinning Disk Confocal Scanner Units (CSUs) real-time live-cell imaging
- Proprietary Microlens-Enhanced Dual Spinning Disk design
- Transform optical microscopes
Utilizing powerful software, high-content analysis (HCA) systems address a wide range of research applications from basic science to complex compound screening.
- Real-time monitoring
- Advanced bioreactor process control
- Bioreactors automate lab-scale mammalian cell cultures
- Detecting viable cell densities and glucose and lactate concentrations with high accuracy
Equipped with a minimally-invasive nanopipette, our Single Cellome UnitTM is capable of injecting target substances while maintaining the positional information of individual cells.
By analyzing particles accurately, reliably, and quickly via automated imaging, FlowCam® technologies advance research, increase productivity, and ensure quality.
Principles of Spinning Disk Confocal
The most common conventional confocal microscopes use a single laser beam to scan a specimen, while the CSU scans the field of view with approximately 1,000 laser beams by using microlens-enhanced Nipkow-disk scanning. In short, CSU can scan 1,000 times faster.
By using a disk containing microlens arrays in combination with the Nipkow disk, we have succeeded in dramatically improving the light efficiency and therefore successfully made real-time confocal imaging of live cells possible.
The expanded and collimated laser beam illuminates the upper disk containing about 20,000 microlenses (microlens array disk). Each microlens focuses the laser beam onto its corresponding pinhole, thus effectively increasing laser intensity through pinholes placed in the pinhole array disk (Nipkow disk).
With the microlens, backscattering of laser light at the surface of the pinhole disk can be significantly reduced, dramatically increasing the signal to noise ratio (S/N) of confocal images.
About 1,000 laser beams passing through each of the pinholes fill the aperture of the objective lens, and are then focused on the focal plane. Fluorescence generated from the specimen is captured by the objective lens and focused back onto the pinhole disk, transmitted through the same holes to eliminate out-of-focus signals, deflected by the dichroic mirror located between microlens array disk and the Nipkow disk to split fluorescence signal from reflected laser, passed through emission filter and then focused into the image plane in the eyepiece or camera.
The microlens array disk and the Nipkow disk are physically fixed to each other and are rotated to scan the entire field of view at high speeds, making it possible to view confocal fluorescent images in real-time through the eyepiece of the CSU head.
Compared to conventional single point scanning, multi beam scanning by the CSU requires a significantly low level of light intensity per unit area, which results in significantly reduced photo bleaching and phototoxicity in live cells.
Spinning Disk Confocal
Microlens-enhanced Nipkow Disk Technology
Comparison of scanning method
1 line scan time＝1[ms]
Scan lines＝1000 [lines]
Disk Scanning by CSU
Rotation Speed＝10000 [rpm]=41.7[rps]
1÷( 41.7×30/360 )＝ 0.5 [ms]
What is a Confocal Scanner Unit?
Confocal scanner unit CSU series enable 3D observation of the cells in detail and dynamics of organelles inside cells. Since the CSU series is capable of high-speed shooting, it is also suitable for observing high-speed life phenomena. In addition, the CSU series is a multi-point confocal method which is extremely gentle to cells, best suitable for long-term live cell observation.
Industrial Applications & Types of Confocal Scanner Units
For pharmaceutical, food, and cosmetic developers
- Evaluation of drug efficacy and toxicity by using cultured cells instead of expensive animal experiments
- Evaluation experiments using cell clusters (spheroids, organoids), which have been extensively researched in recent years
- Evaluation of effects of functional foods on cells
- Efficacy evaluation of cosmetics by using 3D skin model
- Confirmation of stem cell differentiation state and quality evaluation for regenerative medicine
Benefits of Confocal Scanner Units
Yokogawa spinning disk confocal technologies:
- High-efficiency imaging
- Less photobleaching and phototoxicity
- Less expensive
- FOV is 4x wider than other conventional industry models
- Capable of fully automated experiments
- Multiple configurations
- Selectable pinhole size
- World's fastest scanning speed of up to 2,000 fps
- Microlens-enhanced Nipkow disk scanning
- Exchangeable dichroic mirror block and emission filters
- O2 emissions reduced by 40%
single-cell analysis specifically transcription by steroid receptors primarily estrogen and androgen receptors
Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression
world-class team with an unmatched combination of imaging experts, assay development experience and the latest technologies and data analysis capabilities
drug discovery and drug development for an interrelated set of disorders that emanate from type II diabetes and obesity
Faster, Deeper, and Clearer -in vivo molecular imaging technology-
Discovering the Basic Principles of Life through the Live Imaging of C. elegans
Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.
New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!
Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.
Use of the spinning disk confocal at the Harvard Medical School microscopy core.
Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.
On-site manipulation of protein activities: Understanding intricate cell signaling pathways.
A critical requirement in biopharmaceutical development is the integration and automation of process equipment and analytical instruments used in the laboratory. Bioprocess labs with multiple lab-scale bioreactors often execute cultivation experiments in parallel for research or process development purposes.
As part of a collaboration between Securecell (Zurich, SW) and Yokogawa Life Science (Tokyo, Japan), this application note demonstrates the effective use of the Lucullus® Process Information Management System (Lucullus®) to assist in the control of three Advanced Control Bioreactor Systems (BR1000) to study glucose utilization of CHO cells for optimal monoclonal antibody productivity.
Comparison between CSU and conventional LSM in 4D movies.
To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.
The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.
Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.
Caustic soda is an important basic material in the chemical industry and is mainly produced by the electrolysis of soda. In the electrolysis process to make concentrated caustic soda, the DM8 Liquid Density Meter ensures high product quality through accurate measurement of liquid density.
The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.
List of Selected Publications : CSU-W1
List of Selected Publications : CQ1
List of Selected Publications : CSU-X1
List of Selected Publications : CV8000, CV7000, CV6000
Applications: Colony Formation, Scratch Wound, Cytotoxicity, Neurite Outgrowth, Co-culture Analysis, Cell Tracking
Fluorescent ubiquitination-based cell cycle indicator (Fucci) is a set of fluorescent probes which enables the visualization of cell cycle progression in living cells.
Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System
This "Tutorial" provides overview of this software, from installation through data analysis.
In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.
In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.
In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.
In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.
In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.
In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.
In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.
In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.
In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.
In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.
Fast, gentle, and clear - live-cell imaging. Yokogawa's unique scanning method minimizes damage to living cells and organisms and even can capture faint/fast life phenomena.
More than 2,500+ units scanning units sold worldwide. This fast, reliable, and accurate technology has been leading cutting-edge research and supporting researchers around the world for more than two decades.
More information: https://www.yokogawa.com/us/solutions/products-platforms/life-science/spinning-disk-confocal/
#confocal #microlens #microscope #CSU #Yokogawa #livecell
Yokogawa's CQ1 open platform integrates seamlessly with Advanced Solutions BioAssemblyBot® 400. With laboratory automation becoming a standard in research, Yokogawa's high content confocal system's ability to work with robots like Advanced Solutions' BioAssemblyBot® 400 is essential to advancing laboratory automation.
Physiologically relevant 3D cell models are essential for drug discovery and preclinical research due to their functional and architectural similarity to solid tumors. One of the challenges faced by researchers is that many of the assays using these precious samples tend to be manual and tedious.
Using proprietary microfluidics technology, Protein Fluidics has created the Pu·MA System for automated complex 3D cell-based assays. In this webinar, application scientist Dr. Katya Nikolov will present her work on combining this novel automation technology with Yokogawa’s high-content imaging systems for biomarker detection in 3D cell models. Nikolov will demonstrate the utility of an automated immunofluorescence staining workflow followed by confocal imaging within the Pu·MA System flowchips. This automated workflow enables quantitative assessment of biomarkers which provides valuable data for further understanding disease mechanisms, preclinical drug efficacy studies, and in personalized medicine.
This webinar will explore:
- The Pu·MA System and novel technology for automated 3D cell-based assays
- How to perform automated immunofluorescence staining for biomarkers with a “hands-off” assay workflow
- How to visualize biomarkers after the assay with high-content imaging within the flowchip
Physiologically relevant 3D cell models are being adopted for disease modeling, drug discovery and preclinical research due to their functional and architectural similarity to their tissue/sample of origin, especially for oncology research. Multifunctional profiling and assays using 3D cell models such as tumoroids tend to be manual and tedious. Further, high-content imaging of biomarkers in 3D cell models can be difficult.
In this two-part webinar present to you streamlined technologies which can bring consistent timesaving, ease-of-use, and high-quality data to your 3D cell-based workflows:
(A) The Pu·MA System is a microfluidics-based benchtop automated device for performing “hands-off” 3D cell-based assays. In this webinar, application scientist Dr. Katya Nikolov will present data from optimized assays using tumoroids followed by Yokogawa’s high-content imaging systems for biomarker detection.
(B) Yokogawa’s high-content imaging systems such as CellVoyager CQ1 provide superior confocal imaging using the Nipkow Spinning Disk Confocal Technology. Here, application scientist, Dan Collins will present details of the high-content imaging capabilities, easy to use and intuitive image acquisition software, especially for increasing productivity and a streamlined workflow.
- The open platform, Pu·MA System can be used to automate your 3D cell-based assays
- To perform automated IF staining for biomarkers using tumoroid models without perturbing your precious samples
- Image acquisition from 3D cell models using Yokogawa’s high-content imaging platforms
- Image analysis from cells, complex spheroids, colonies, or tissues using the CellPathfinder high content analysis software
3D imaging experts from Yokogawa and Insphero have come together to provide helpful tips and tricks on acquiring the best 3D spheroid and organoid imaging. This webinar focuses on sample preparation, imaging, and analysis for both fixed and live cells in High Content Screening assays. The experts also discuss automated tools that can help researchers understand the large volume of data in these High Content Imaging Analysis Systems.
In this webinar, Professor Jonny Sexton discusses a pipeline, developed in the Sexton lab, for the quantitative high-throughput image-based screening of SARS-CoV-2 infection to identify potential antiviral mechanisms and allow selection of appropriate drug combinations to treat COVID-19. This webinar presents evidence that morphological profiling can robustly identify new potential therapeutics against SARS-CoV-2 infection as well as drugs that potentially worsen COVID-19 outcomes.
Human pluripotent stem cells are proven efficient models for drug screening campaigns. They provide access to unlimited starting material amenable to high throughput small molecule compound screening. Due to their capacity to generate mode complex cellular models, they also offer the potential to perform high content screening in tissue-specific organoids for further human target validation.
Dr. Alejandro Hidalgo-Gonzalez at MCRI(Murdoch Children's Research Institute) in Australia is a user of our HCA system CellVoyager CV8000 and he has established a pipeline for assay development and automated unbiased phenotypic drug screening using human pluripotent stem cell-derived cells and organoids. This is a recording of his presentation at an educational webinar organized by A*STAR in Singapore.
Visualizing the complex spatiotemporal dynamics of human stem cells as they proliferate and make cell fate decisions is key to improving our understanding of how to robustly engineer differentiated tissues for therapeutic applications.
In this webinar, Dr. Rafael Carazo Salas will describe multicolor, multiday high-content microscopy pipelines that his group has recently developed to visualize the dynamical cell fate changes of human Pluripotent Stem Cells (hPSCs).
- Visualizing how human Pluripotent Stem Cells (hPSCs) proliferate and undergo early differentiation in vitro, by high content microscopy
- Learning about experimental and computational pipelines that enable monitoring single-cell fate dynamics
- Learning about novel “live” reporters of hPSC cell fate
Rafael Carazo Salas, PhD
Professor, School of Cellular and Molecular Medicine
University of Bristol
This webinar highlights Yokogawa’s High Content Solutions, the benchtop confocal CellVoyager CQ1, and CellVoyager CV8000. Utilizing Yokogawa’s dual-wide microlens spinning disk confocal technology, these automated HCA systems provide remarkable image quality while increasing your output. This frees up time to complete other research activities. Also, recent additions to the CSU-W1 confocal upgrade is discussed. The SoRa, a super-resolution solution, and the Uniformizer, an image flattening device. Both of which can be added to the lightpath of your CSU-W1-enhanced microscope.
Introduction to Yokogawa
SoRa for CSU-W1 super-resolution with confocal
Two high content instruments from Yokogawa: The CQ1 and the CV8000
Dan J. Collins, Applications Scientist, Yokogawa Life Science
Dr. Sexton discusses high content screening for phenotypic-based drug discovery and development using Yokogawa technologies. This webinar presents the methodology behind acquiring good images that are able to leverage the three-dimensionality of different cell systems. His assays include 3D models such as organoids and spheroids.
In this webinar, you will discover:
- How to identify when 2D or 3D methods are required to achieve desired results.
- How to leverage your High Content Imaging Systems to get optimal signals and backgrounds.
- Techniques that are used to improve cell observation yield and statistical distributions of morphological features.
In the last few decades, the pharmaceutical industry has transformed people’s lives. However, the development of new drugs is becoming increasingly difficult and a paradigm shift in the drug discovery workflow is required to reduce attrition and transform conventional drug screening assays into translatable analytical techniques for the analysis of drugs in complex environments, both in-vitro and ex-vivo. The ability to visualize unlabelled compounds inside the cell at physiological dosages can offer valuable insight into the compound behavior both on and off-target.
SiLC-MS is a semi-automated methodology that allows the collection of intracellular contents using a modified CQ1 imaging system developed by Yokowaga. The instrument is equipped with a confocal microscope that allows bright field imaging as well as fluorescence imaging with 4 lasers (405, 488, 561, and 640 nm). Sampling is performed using the tips developed by Professor Masujima (1-4).
In this study, we show the applicability of the SiLC-MS technology to drug discovery, as it is crucial to identify compound and its metabolites when incubated in a mammalian cell at a therapeutic dose. We report on the validation studies performed using the SiLC-MS platform, in these validation studies we assess the ability to distinguish different cell types based on their metabolomic fingerprint, furthermore, we have also evaluated if this assay was sensitive enough to detect drugs intracellularly.
Presenter: Carla Newman, Scientific Leader (Celluar Imaging and Dynamics), GSK
Image-based phenotypic screening relies on the extraction of multivariate information from cells cultured in a large number of screened conditions. In this webinar, we explored the application of complex and biologically relevant model systems for drug screening, such as small intestinal organoids.
Key topics include:
- Learn how to upscale, streamline, and automate intestinal organoid handling
- Learn how to image in complex three-dimensional (3D) model systems and how to approach large imaging datasets
- Understand the basics of multivariate analysis on image-inferred features
Generating translatable high-content imaging data from physiologically-relevant cell models, including 2D and 3D structures, is extremely valuable for drug discovery and pre-clinical research. In this webinar, James Evans, CEO of PhenoVista Biosciences presents case studies on how Yokogawa’s Benchtop CQ1 Confocal System can improve throughput and standardize processes for complex 3D cell-based phenotypic assays.
Key learning objectives:
- Strategies for designing and implementing high-content screening assays
- Approaches for deciding between 2D and 3D model systems
- Supporting drug development research through the use of HCA and three-dimensional culture models -
- Low invasiveness enables single-cell injection of target substances and extraction of intracellular materials -
- For improved efficiency in drug discovery and biological and medical research through the uniform management of microscopic images -
- For the faster discovery of new drugs and improved efficiency in biomedical research -
Looking for more information on our people, technology and solutions?Contact Us