我们的显微镜和生命科学解决方案旨在支持从基础研究到药物发现以及临床前试验的各种应用。
横河电机的高含量分析系统和双转盘共聚焦技术用于再生医学、药物研究和精准医学,提供了高速、高分辨率的活细胞成像。
-
转盘共焦CSU
作为双转盘技术的先驱,横河电机的共焦扫描仪单元实现了实时活细胞成像技术,改变了光学显微镜。
-
高内涵分析
我们的高内涵分析系统利用强大的分析软件解决从基础科学到药物发现筛选的一系列研究应用。
-
生物反应器
用自动化取代手动工艺是生物制药行业的一种趋势。为了实现分批补料哺乳动物细胞培养的完全自动化,葡萄糖(一种关键的营养来源)的控制至关重要。通过在线传感和模型预测控制软件,可以实现生物反应器的自动进料和葡萄糖的浓度稳定。
-
单细胞分析解决方案 Single Cellome™
我们正在开发单细胞和活细胞的细胞处理技术。SU10 提供选择性、破坏性最小的自动化纳米点传送。SS2000 基于共聚焦显微镜技术提供自动亚细胞取样。
-
FlowCam 流式成像颗粒分析系统
使用FlowCam,您可以准确、可靠、快速地分析粒子,借助自动成像技术推进您的研究,提高生产力并确保质量。
-
OpreX Informatics Manager
横河电机的OpreX Informatics Manager(信息管理系统)是一种信息集成解决方案。它可在技能和调度方面优化人力和物力资源管理,超越了电子实验室笔记本。
详细介绍
我们将相关信息发布到以下 SNS上面。
请关注我们。
横河生命科学
@Yokogawa_LS | |
Yokogawa Life Science | |
Yokogawa Life Science | |
•YouTube | Life Science Yokogawa |
横河官方社交媒体
转盘共焦原理
最常见的传统共焦显微镜使用单个激光束扫描标本,而CSU使用约1,000束激光束扫描视野,通过使用微透镜增强的Nipkow圆盘扫描:CSU的扫描速度可以提高 1,000 倍。
通过将包含微透镜阵列的磁盘与Nipkow磁盘结合使用,我们显著提高了光效率,成功地使活细胞的实时共焦成像成为可能。
强烈而笔直的激光束照亮包含大约20,000个微透镜(微透镜阵列盘)的上盘。 每个微透镜将激光束聚焦到其相应的针孔上,从而通过针孔阵列盘(Nipkow 盘)中的针孔有效地增加激光强度。
使用微透镜,可以显着减少激光在针孔盘表面的反向散射,从而显着提高共焦图像的信噪比 (S/N)。
大约1000束激光穿过每个针孔,填满物镜的光圈,然后聚焦在焦平面上。样品产生的荧光被物镜捕获并聚焦回针孔盘上,通过相同的孔传输以消除失焦信号,由位于微透镜阵列盘和Nipkow盘之间的二向色镜偏转,从反射的激光中分离出荧光信号,通过发射过滤器,聚焦到目镜或相机的图像平面。
微透镜阵列盘和 Nipkow 盘在物理上相互固定,通过旋转高速扫描整个视野,因此,可以通过CSU头的目镜实时查看共焦荧光图像。
与传统的单点扫描相比,CSU的多光束扫描对单位面积的光强度要求非常低,这使活细胞中的光漂白和光毒性显著降低。
转盘共焦
微透镜增强 Nipkow 磁盘技术
扫描方式比较

单点扫描
1 line scan time=1[ms]
1000 lines/image
Scan lines=1000 [lines]
1×1000=1000 [ms]

CSU磁盘扫描
Rotation Speed=10000 [rpm]=41.7[rps]
30°Rotation/image
1÷( 41.7×30/360 )= 0.5 [ms]
二月 | 18,2022 |
销售发布 : Single CellomeTM System SS2000 我们将于2022年2月18日发售Single Cellome System SS2000 优点
发布日期 请联系我们了解详情。 |
二月 | 15,2022 |
销售发布:共焦定量细胞成像仪 CellVoyager CQ1 新选项和最新软件发布
|
二月 | 15,2022 |
销售发布:CellPathfinder 新产品和最新软件发布
|
十二月 | 1,2021 |
横河电机开发出用于亚细胞采样的单细胞系统SS2000 <细胞采样> <SS2000的特点> SS2000将于2022年2月在日本、美国和中国发售,随后还将在欧洲等其他市场发售。 |
十一月 | 18,2021 |
横河电机收购创新型生物过程数字孪生技术开发商Insilico Biotechnology公司 横河电机公司收购生物过程软件和服务技术开发商 Insilico Biotechnology公司 |
一月 | 8,2021 |
销售发布: 先进控制生物反应器系统BR1000 |
八月 | 20,2020 |
利用高容量筛选探索潜在的Covid-19治疗方法 |
六月 | 5,2020 |
Sale新闻发布 : 高容量细胞发现系统 CV8000:发布 20 倍水浸透镜选项。 |
三月 | 18,2020 |
新闻发布 : 单细胞分析解决方案 Single Cellome Unit SU10 |
一月 | 20,2020 |
新闻发布 : 发布了高容量分析软件 CellPathfinder 升级和深度学习功能选项。 产品链接 High Content Analysis Software CellPathfinder |
January | 15,2020 |
实验室自动筛选协会 (SLAS) 2020 一月 25-29, 2020 我们将展示高容量高含量分析系统 "CellVoyager CQ1". 产品链接 -海报- 1207-C: |
参考
Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression
Faster, Deeper, and Clearer -in vivo molecular imaging technology-
Discovering the Basic Principles of Life through the Live Imaging of C. elegans
Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.
New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!
Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.
Use of the spinning disk confocal at the Harvard Medical School microscopy core.
Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.
On-site manipulation of protein activities: Understanding intricate cell signaling pathways.
Comparison between CSU and conventional LSM in 4D movies.
To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.
CV1000 clears the hurdle in Live Cell Imaging
All-in-one Live cell imaging solution
The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.
Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.
The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.
List of Selected Publications : CSU-W1
List of Selected Publications : CQ1
List of Selected Publications : CSU-X1
List of Selected Publications : CV8000, CV7000, CV6000
- Colony Formation
- Scratch Wound
- Cytotoxicity
- Neurite Outgrowth
- Co-culture Analysis
- Cell Tracking
Faster, Brighter, and More Versatile Confocal Scanner Unit
Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System
Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer
Wide and Clear
Confocal Scanner Unit
This "Tutorial" provides overview of this software, from installation through data analysis.
In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.
In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.
In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.
In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.
In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.
In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.
In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.
In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.
In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.
In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.
下载
视频
The Yokogawa business vision states that the company endeavors to achieve Net-zero emissions, ensure the Well-being of all, and make a transition to a Circular Economy by 2050.
YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.
YOKOGAWA aspires to establish Smart GMP manufacturing facilities that provide consistent quality and supply while eliminating industrial waste, enhancing productivity and always using high-quality component parts and materials.
YOKOGAWA creates autonomous operations with high-efficiency automation and optimization that allows growth with minimal deployment of manpower.
新闻
-
新闻 2017年8月7日 横河电机制定面向2050年的可持续发展目标
- 通过联合创新创造新价值,为解决全球性问题做贡献 -
-
新闻 2019年11月13日 横河电机收购美国公司的纳米移液管技术,应用于生命科学领域
- 其微创性使在单细胞内注射目标物质和提取细胞内物质成为可能 -
-
新闻 2020年6月5日 CSU-W1共焦扫描仪抵达国际空间站
-
新闻 2020年12月3日 横河与InSphero签订合作协议
- 通过使用HCA和三维培养模型支持药物研发 -
想要了解更多的信息,技术&解决方案?
联系我们