CSU-W1 Applications

Application:  CSU-W1

• ES cell
  • Ocular cup organ regenerated from mouse ES cells
  • ES cell colony
• Zebrafish
  • Zebrafish embryo
  • Zebrafish
• Brain slice
  • Brain slice of mouse fetus
• Early stage embryo
  • Early stage mouse embryo

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ES cell

Ocular cup organ regenerated from mouse ES cells

Data:  Mototsugu Eiraku, Ph.D., and Yuiko Hasegawa, Ph.D., Sasai Lab., Organogenesis Neurogenesis group, Center for Developmental Biology, RIKEN

Measurement condition
Z-section/stack	100um（2um/51slices)
Fluorescent probe	640nm:Cy5
Pinhole	25um
Objective lens	20x dry

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ES cell colony

Data:  Nozomu Takata, Ph.D., Sasai Lab.,Organogenesis Neurogenesis group, Center for Developmental Biology, RIKEN

Measurement condition
Z-section/stack	50um（1um/51slices)
Fluorescent probe	GFP（Excitation：488nm） mCherry（Excitation：561nm
Pinhole	50um
Objective lens	60x oil

Data:  Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University

Measurement condition
Z-sections/ stack	80um （0.4um/201slices)
Fluorescent probe	488nm： H2B-EGFP 561nm: mCherry-MBD-NLS
Pinhole	50um
Objective lens	60x silicone
Total time	490 mins (Interval：10mins)

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Zebrafish

 

Zebra fish embryo

Data:  Makoto Suzuki, Ph.D. and Naoto Ueno, Ph.D.,Division of Morphogenesis, National Institute of Basic Biology

Measurement condition
Z-Sections/ stack	Upper left:99um（1um/100slices), Lower left:50um（0.5um/101slices), Right:1slice
Fluorescent probe	Left:membrane RFP（Excitation：561nm） Right:EB3-GFP（Excitation：488nm） membrane RFP（Excitation：561nm）
Pinhole	50um
Objective lens	Upper left:20x dry,Lower left,right: 60x water

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Zebrafish

Real-Time Visualization of Neuronal Activity

Data:  Akira Muto and Koichi Kawakami, Division of Molecular and Developmental Biology, National Institute of Genetics

Measurement condition
Total time	152 sec. (Stream : 600 images)
Excitation	488nm:GCaMP
Pinhole	50um
Objective lens	40x water

Reference:  Muto et al., Real-Time Visualization of Neuronal Activity during Perception, Current
Biology 23(4):307-311

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Brain slice

Brain slice of mouse fetus

Data:  Atsunori Shitamukai, Ph.D., Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN

Measurement condition
Z-sections/stack	15um（0.5um/31slices)
Fluorescent probe	GFP（Excitation：488nm） RFP（Excitation：561nm）
Pinhole	50um
Objective lens	60x water LWD
Total time	2hours (Interval：1min)

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Early stage embryo

Early stage mouse embryo

Data:  Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University