CSU-W1 Applications

Application:  CSU-W1


ES cell

Ocular cup organ regenerated from mouse ES cells ES cell colony ES cell colony

Ocular cup organ regenerated from mouse ES cells

Data:  Mototsugu Eiraku, Ph.D., and Yuiko Hasegawa, Ph.D., Sasai Lab., Organogenesis Neurogenesis group, Center for Developmental Biology, RIKEN

Ocular cup organ regenerated from mouse ES cells

  3D Play

Ocular cup organ regenerated from mouse ES cells MIP

YZ plane YZ plane

Measurement condition
Z-section/stack 100um(2um/51slices)
Fluorescent probe 640nm:Cy5
Pinhole 25um
Objective lens 20x dry

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ES cell colony

Data:  Nozomu Takata, Ph.D., Sasai Lab., Organogenesis Neurogenesis group, Center for Developmental Biology, RIKEN

ES cell colony

3D Play

ES cell colony MIP

Measurement condition
Z-section/stack 50um(1um/51slices)
Fluorescent probe GFP(Excitation:488nm)
mCherry(Excitation:561nm
Pinhole 50um
Objective lens 60x oil

Data:  Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University

 ES cell colony

Timelapse(MIP)

Full-size movie Play

Measurement condition
Z-sections/
stack
80um
(0.4um/201slices)
Fluorescent probe 488nm:
H2B-EGFP
561nm:
mCherry-MBD-NLS
Pinhole 50um
Objective lens 60x silicone
Total time 490 mins
(Interval:10mins)

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Zebrafish

Zebrafish embryo Zebrafish

Zebra fish embryo

Data:  Makoto Suzuki, Ph.D., and Naoto Ueno, Ph.D., Division of Morphogenesis, National Institute of Basic Biology

Zebra fish embryo

3D reconstructed image of the whole embryo

Zebra fish embryo

Excerpts from Timelapse(MIP)
All timelapse Play

Zebra fish embryo

3D reconstructed embryo
(partial, at high magnification)

YZ Plane

YZ Plane

Measurement condition
Z-Sections/
stack
Upper left:99um(1um/100slices),
Lower left:50um(0.5um/101slices),
Right:1slice
Fluorescent probe Left: membrane RFP(Excitation:561nm)
Right: EB3-GFP(Excitation:488nm)
membrane RFP(Excitation:561nm)
Pinhole 50um
Objective lens Upper left:20x dry,Lower left,right:
60x water

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Zebrafish

Real-Time Visualization of Neuronal Activity

Data:  Akira Muto and Koichi Kawakami, Division of Molecular and Developmental Biology, National Institute of Genetics

zebra fish

Excerpts from Timelapse(MIP)
All timelapse movie: Play

zebra fish

Time-line MIP of timelapse images

zebra fish

3D

zebra fish

Larval zebrafish: The fifth day after fertilization
Pink area: Optic tectum

Measurement condition
Total time 152 sec. (Stream: 600 images)
Excitation 488nm: GCaMP
Pinhole 50um
Objective lens 40x water

Reference:  Muto et al., Real-Time Visualization of Neuronal Activity during Perception, Current
Biology 23(4):307-311

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Brain slice

Brain slice of mouse fetus

Brain slice of mouse fetus

Data:  Atsunori Shitamukai, Ph.D., Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN

Brain slice of mouse fetus

3D

Measurement condition
Z-sections/stack 15um(0.5um/31slices)
Fluorescent probe GFP(Excitation:488nm) RFP(Excitation:561nm)
Pinhole 50um
Objective lens 60x water LWD
Total time 2hours (Interval:1min)

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Early-stage embryo

Early stage mouse embryo

Early-stage mouse embryo

Data:  Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University

Early stage mouse embryo

Timelapse (MIP)  |  Full-size movie Play

Measurement condition
Z-sections/
stack
100um
(1um/101slices)
Fluorescent probe 488nm:
H2B-EGFP
561nm:
mCherry-MBD-NLS
Pinhole 50um
Objective lens 60x silicone
Total time 48 hours
(Interval:10mins)
 Early stage mouse embryo

Excerpts from Timelapse (MIP of chromosome)

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Industries

  • Pharmaceutical

    The pharmaceutical industry currently faces a major challenge in taking full advantage of the opportunities presented in large emerging markets. Now, more than ever, pharmaceutical companies need to introduce lean manufacturing techniques that will enhance profitability. As one of the world's leading industrial automation suppliers, Yokogawa is committed to delivering the best possible solutions for your best manufacturing practices.

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  • Life Science

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