CellVoyager CV8000 High-Content Screening System

The CV8000 features a cell incubator with an improved airtight design that facilitates the observation of cell behavior over long periods of time. In addition, the CV8000 comes with CellPathfinder, a new program that can analyze images of unlabeled cells and 3D images of samples. With these features, the CV8000 improves the efficiency of drug discovery research and biomedical research on leading-edge subjects such as iPS and ES cells.

CV8000 System Highlights

Excitation laser wavelength 405 nm, 445 nm, 488 nm, 561 nm, 640 nm
Illumination source Laser
Objective lens 2x to 60x
(Dry, Phase contrast, Water immersion, Long working distance)
Camera High-sensitivity sCMOS camera (up to 4 units)
Autofocus Laser-based mode, image-based mode
Software CellPathfinder, CellLibrarian

Observe label-free cells

Using the digital phase contrast (DPC) method, the CV8000 enables observation of label-free cells, providing high-contrast images and detailed cell information.

Capture more images in less time

The CV8000 has four cameras to increase the number of simultaneously captured images, shorten the image acquisition time, and obtain multicolor imaging.

Obtain clear, high-definition images

Equipped with Yokogawa’s original confocal scanning disk technology based on the Nipkow disk, the CV8000 facilitates observation of cells with weak fluorescence intensity.

 

 

 

Over twenty years of experience

  • Yokogawa CSU confocal scanner unit has been selected by top scientists, and more than 2800 units*1 have been sold in the world.
  • High-speed and low-phototoxic imaging are achieved by microlens-enhanced Nipkow disk system.
     

25/50 μm pinhole disk exchanger※2.

  • Users can choose the pinhole disk matching to the purpose of the experiments.
  • Z-resolution in thick samples is improved by using 25 μm pinhole disk

    .


*1  As of September 2017

*2  Option
 

Highly accurate stage incubator and dispenser for time lapse

  • Stage incubator enables live cell imaging over 3 days without supplying additional water※1
  • Disposable tip type liquid dispenser for kinetic assays※2
  • Hypoxia experiments and FRET

Stage incubator performance

Calcium response

 

Fast kinetic responses can be visualized by applying drugs during time lapse recordings.
Ionomycin was applied to A10 cells labeled with Fluo-4 during recording with 100ms intervals.

High throughput

  • Wide-view sCMOS camera, up to 4 cameras
  • Approx. 1 minute to acquire 4-color images of 96 wells※3

Water immersion objectives

  • 40x and 60x water immersion objectives provide brighter and higher resolution images.
  • Automated water supply


※1 Depends on conditions and samples.
※2 Optional
※3 1 field/well, 4-camea simultaneous recording

High content analysis system CellPathfinder   Click Here For More Info!

  • Preset analysis menus for a variety of applications
  • Flexible graph functions to display analysis results
  • Direct link between chart and object imageMachine learning

Machine learning


Software learns the features of the sampleobjects collected by users.

3D analysis


 

Label-free analysis
DPC※1 function is a powerful tool to analyze unstained bright field samples.

※1 Digital phase contrast

High-throughput Cytological Discovery System

Model CV8000
Sample format Multiple well plate (6, 12, 24, 48, 96, 384, 1536 wells), glass slide
Image mode Confocal mode
max. 4 color simultaneous recording,
Brightfield mode, Phase-contrast
(10x, 20x for 6, 12, 24 well plates)
digital phase contrast (10x 20x)
Output data format Image data: 16bit TIFF, PNG
Numerical data: CSV, Original format
Excitation wavelength 405/445/488/561/640nm, all solid laser, max. 5 lasers
【Option】365nmLED
White light illumination LED
Autofocus Laser-based mode, Image-based mode
Objective lens Max. 6 lenses are available : Automatically switchable
Dry: 2x, 4x, 10x, 20x, 40x
Water immersion: 40x, 60x
Long working distance: 20x
Phase-contrast: 10x, 20x
Confocal unit Microlens-enhanced wide-view dual Nipkow disk confocal scanner, 50 μm pinhole
【Option】 25/50 μm pinhole disk exchanger
Camera sCMOS (effective pixels: 2000X2000 pixel size: 6.5 μm), max. 4 cameras
Stage incubator Temperature: 35-40℃ CO2 supply box (CO2: 5%, forced humidification)
Dispenser 【Option】Disposable tip type
(For 96 tip , 384 tip type)
Bar-code reader 【Option】1 or 2 dimension
Workstation Dual-monitor workstation for system control
Dual-monitor workstation for data analysis
Analysis software
(CellPathfinder)
Granularity, Neurite, Nuclear morphology, Nuclear translocation, Plasma membrane translocation, Machine learning, Label-free analysis, 3D analysis, Texture analysis, etc.
Operating environment 15-30ºC, 20-70%RH (No condensation)
Power supply Measurement unit: Single-phase 200VAC, 2KVAmax
Workstation for system control : 100VAC, 1.3KVAmax, Workstation for data analysis: 100VAC, 690VAmax
External dimension Measurement unit: 1280W×895D×1450H mm
Weight Measurement unit: 510Kg

CV8000 Partner

North America, Europe

WAKO Automation USA, Inc.

WAKO Automation USA, Inc.
Recommended vendor for High Content Screening integration and automation solutions.

 

Asia

Tekon Biotech

Tekon Biotech
P.R.China, Hong Kong, Taiwan, Macao


Shanghai Genesci Medical technology

Shanghai Genesci Medical technology Co.,Ltd.
People's Republic of China (excluding Hong Kong, Taiwan, Macao)


LBD Life Sciences China Limited

LBD Life Sciences China Limited.
People's Republic of China (excluding Hong Kong, Taiwan, Macao)

CellPathfinder

CellPathfinder is designed for our HCA systems, CQ1 and the CellVoyager series. From beginners to experts, the analysis software lets you quantify subtle physiological changes and even label-free samples with various graph

CellLibrarian

CellLibrarian is a powerful solution to manage a volume of image data acquired by Yokogawa CellVoyager series.

Overview:

The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

Overview:

We have been developing a prototype of a genomic drug test support system using our CSU confocal scanner. This system administers chemical compounds that serve as potential drug candidates into living cells, which are the most basic components of all living organisms, records the changes in the amount and localization of target molecules inside cells with the CSU confocal scanner and a highly sensitive CCD camera, and processes and quantifies the captured high-resolution image data.

Yokogawa Technical Report
2.2 MB

Product Overviews

    Product Overview
    Overview:

    The CV8000 features a cell incubator with an improved airtight design that facilitates the observation of cell behavior over long periods of time. In addition, the CV8000 comes with CellPathfinder, a new program that can analyze images of unlabeled cells and 3D images of samples. With these features, the CV8000 improves the efficiency of drug discovery research and biomedical research on leading-edge subjects such as iPS and ES cells.

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