CQ1 Benchtop High-Content Analysis System

CQ1 enables 3D imaging and quantification of live cell clusters, such as spheroids within a 3D culture vessel, as they are, keeping the cells intact. CQ1 exports feature data in general formats which are readable by various third-party software for advanced data analysis. It is possible to construct fully customized CQ1-based system by integrating with external systems*1, via robot for culture dish handling.

CQ1 System Highlights

Excitation laser wavelength 405 nm, 488 nm, 561 nm, 640 nm
Illumination source Laser
Objective lens 2x to 60x
(Dry, Phase contrast, Long working distance)
Camera High-sensitivity sCMOS camera
Autofocus Laser autofocus, Software autofocus
Software CellPathfinder

Enables measurement of spheroids, colonies and tissue sections

  • No need to remove cells from culture dish, in contrast to traditional flow cytometry
  • Nipkow spinning disk confocal technology allows high-speed yet gentle 3D image acquisition
  • Rich feature extraction to facilitate sophisticated cellular image analysis
  • Wide field of view and tiling capability enable easy imaging of large specimen

Enables analysis of time lapse and live cell

  • High precision stage incubator and low phototoxicity of our confocal makes the analysis of time lapce and live cell are possible   
  • Max.20fps option for fast time lapce*1
CQ1

High quality image and the similar operability to traditional flow cytometer

  • Feature data and statistical graphs displayed in real-time with image acquisition
  • Usuable high quality image as confocal micorscope image.
  • Easy to trace back to the original image from a graph spot, and make repetitive measurements

Open platform

  • Connectable with external systems via handling robot*2
  • Expandable to integrated system as image acquisition and quantification instrument
  • FCS/CSV/ICE data format readable by third-party data analysis software
  • A variety of cell culture and sample dishes are applicable

Compact footprint, light weight bench-top device; no need for darkroom

  CQ1 General fluorescent imaging Flow cytometry
Cell removal/suspension treatment Not necessary Not necessary Necessary
Cell image confirmation Possible Possible Not possible
Display feature data and graphs in real-time with imaging Possible Depends on devices Possible
3D data measurement Possible Not possible Not possible
Time lapse Possible Not possible Not possible

*1 Option
*2 Contact to CQ1 partner for more information

We post our information to the following SNSs.
Please follow us.

 

Yokogawa Life Science

•Twitter @Yokogawa_LS
•Facebook Yokogawa Life Science
•LinkedIn Yokogawa Life Science

 

Yokogawa's Official Social Media Account List

Social Media Account List

Multiple functions fully integrated in a compact box

Compact design contains fully integrated multiple functions to offer easy-to-handle confocal imaging system, without a need for complicated system integration; you only need to set a sample and run the software. User-friendly interface and versatile functions support your measurement and analysis.

CQ1

Principles of the Microlens-enhanced Nipkow Disk Scanning Technology

A Nipkow spinning disk containing about 20,000 pinholes and a subsidiary spinning disk containing the same number of microlenses to focus excitation laser light into each corresponding pinhole are mechanically fixed on a motor, and very rapidly rotated. As a result, high-speed raster scan of the excitation lights on the specimen can be achieved.The pinhole and microlenses are arranged on each disk in our proprietary design to optimize raster scan. Multi-beam scanning not only increases scanning speed, but also results in significantly lower photobleaching and phototoxicity, because multi-beam excitation needs only low level of laser power on the specimen to fully excite fluorescence.

CQ1

System integration with CQ1

System integration with CQ1

Measurement Procedure

Analysis software (Option)

High content analysis system CellPathfinder*1        Click Here For More Info!

  • Preset analysis menus for a variety of applications
  • Flexible graph functions to display analysis results
  • Direct link between chart and object imageMachine learning

Machine learning


Software learns the features of the sampleobjects collected by users.

3D analysis


 

Label-free analysis
DPC*2 function is a powerful tool to analyze unstained bright field samples.

*1 Optional software
*2 Digital phase contrast

Example of setup

Example of setup

 

Item Specifications
Optics Microlens enhanced dual wide Nipkow disk confocal,
Phase contrast (Optional add-on)
Laser/Filter Laser : Choose 2-4 lasers from 405/488/561/640nm,
10-position Filter wheel (built-in)
Camera sCMOS 2560×2160pixel, 16.6×14.0mm
Objective lens Max.6 lenses
(Dry: 2x, 4x, 10x, 20x, 40x Long working distance: 20x, 40x
Phase contrast: 10x, 20x )
Sample vessel Microplate (6, 24, 96, 384 well), Slide glass,
Cover glass chamber*1, Dish (35, 60mm*1)
XY stage High-precision XY stage, designated resolution 0.1µm
Z focus Electric Z motor, designated resolution 0.1µm
Autofocus Laser autofocus, Software autofocus
Feature data Number of cells/cellular granules, Intensity, Volume, Surface area, Area, Perimeter, Diameter, Sphericity, Circularity, etc
Data format Image : 16bit TIFF file (OME-TIFF), PNG
Numerical data : FCS, CSV, ICE
Workstation Measurement and analysis workstation
Size/weight Main unit : 600×400×298mm 38kg
Utility box : 275×432×298mm 18kg
Environment 15 - 30oC、20 - 70%RH No condensation
Power consumption Main unit and Utility box : 100-240VAC 800VAmax, Workstation : 100-240VAC 650VAmax

*1 Under development *2 Display is not included with CQ1 system

Europe

Cenibra

Cenibra GmbH
Germany, Switzerland, Austria and Netherlands


Image Solutions, Ltd.

Image Solutions(UK), Ltd. 
UK and Ireland


Proteigene

Proteigene,
France



Accela s.r.o.,

Accela s.r.o.,,
Czech, Slovak, Hungary, Romania and Bulgaria


Kem-En-Tec Nordic A/S

Kem-En-Tec Nordic A/S,
Denmark, Sweden, Finland and Norway


OPTEC

Optec GmbH
Russia, Ukraine, Kazakhstan, Belarus, Azerbaijan, Armenia, Georgia, Uzbekistan, Turkmenistan, Kyrgyzstan, Tajikistan, and Moldova


North America

Asia

Genesci

Shanghai Genesci Medical technology Co.,Ltd.
People's Republic of China (excluding Hong Kong, Taiwan, Macao)


Tekon Biotech

Tekon Biotech
People's Republic of China, Hong Kong, Taiwan, Macao


JCBIO CO.,LTD

JCBIO CO., LTD
Republic of Korea (South Korea)

Collaborator

De Novo Software

De Novo Software
(product name: FCS Express Image Cytometry)

 

FCS Express Image Cytometry

De Novo Software has been developing flow cytometer data analysis solutions since 1998. Our flagship product, FCS Express™, is world-renowned as a robust, and easy to use flow and image cytometry data analysis application. De Novo Software offers a dedicated image analysis and reporting package for Image Cytometry to improve your workflow and results while giving you access to single cell results evening with high content screening data. FCS Express Image cytometry is directly compatible with the Yokogawa CQ1 quantitative image cytometer through the .ICE file format which enables quick import, analysis, and reporting of your results in FCS Express.

CellPathfinder

CellPathfinder is designed for our HCA systems, CQ1 and the CellVoyager series. From beginners to experts, the analysis software lets you quantify subtle physiological changes and even label-free samples with various graph options.

CellActivision

CellActivision uses Machine Learning Technology and a unique digital filter to recognise cells or colonies directly from label free images. CellActivision can also classify and quantify these cells by the use of sample libraries which are easily prepared by the user.

Application Note
Application Note
Application Note
Overview:

The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

Overview:

List of Selected Publications : CQ1

Yokogawa Technical Report
2.2 MB
Overview:

Instrumentation devices and equipment serve for plants as the eyes, ears, nose, tongue, and skin, which are responsible for the five senses, and act as the central nervous system and brain do for human beings. The reliability and availability of such devices and equipment are the most important requirements of customers.

Looking for more information on our people, technology and solutions?


Contact Us
Top