Anticancer drug screening in 2D/3D cultures using cell cycle marker Fucci
Fucci(CA)5 is a new variant of Fluorescent Ubiquitination-based Cell-Cycle Indicator that allows observation of cell cycle progression in live and fixed samples. The cell cycle phase is visualized by utilizing the ubiquitination domains of human Cdt1 (hCdt1(1/100)Cy(-)) fused with a red fluorescent protein (AzaleaB5), and human Geminin (hGem(1/110)) fused with a green fluorescent protein (h2-3), respectively. Because these red and green probes are ubiquitinated and degraded only in S and G1 phases, respectively, Fucci(CA)5 shows red fluorescence in G1 phase nuclei, green in S phase nuclei and yellow (red + green) in G2-M phase nuclei.
This application note presents an example using HepG2 cells that express Fucci(CA)5 to observe the response of drugs such as anticancer agents with a high content analysis system. Comparing data obtained from 2D and 3D culture conditions has emphasized the importance of understanding spatio-temporal data to study drug effects.
For further information, download the application note at the top of the page.
Reference: Sakaue-Sawano A, Miyawaki A. Mol Cell. 2017. DOI：10.1016/j.molcel.2017.10.001
We post the latest information about life science on social media.
|Follow us||Share our application|
|@Yokogawa_LS||Share on Twitter|
|Yokogawa Life Science||Share on Facebook|
|Yokogawa Life Science||Share on LinkedIn|
Yokogawa's official account list
Gerelateerde producten & oplossingen
Benchtop CQ1 Confocal System
The CellVoyager CQ1 provides the highest quality confocal images and extended live-cell imaging in a space-saving benchtop design.
CellPathfinder is designed for our HCA systems, CQ1 and the CellVoyager series. From beginners to experts, the analysis software lets you quantify subtle physiological changes and even label-free samples with various graph options.
CV8000 High-Throughput System
CellVoyager CV8000 is the most advanced high-content screening system. The improved built-in incubator lets you analyze extended live cell responses. With its expandability, 4 cameras, 5 lasers and an optional built-in pipettor, the system permits increasingly complex assay development and high-content screening.
Yokogawa’s high content analysis systems and dual spinning disk confocal technologies provide high-speed and high-resolution live cell imaging, enabling leading-edge research around the world.
High Content Analysis CellVoyager
Onze high-content analysesystemen (HCA) maken gebruik van krachtige software om een breed scala aan onderzoekstoepassingen aan te pakken, van basiswetenschap tot complexe samengestelde screening.
Contact a Yokogawa Expert to learn how we can help you solve your challenges.