CellVoyager High-Content Analysis System CQ3000 is a high-content analysis platform capable of rapidly acquiring high-resolution 3D images while culturing cells. By combining various options according to each application, it provides a system optimized for your specific research needs.
It enables advanced assays in environments closer to the human body such as organoids—which replicate certain structures and functions of human organs— or organ-on-a-chip models that mimic physiological functions of organs on microfluidic chips. In addition, multicolor imaging using multiple fluorescent dyes allows complex assays like morphological profiling of cellular components and organelles through techniques such as Cell Painting.
High Throughput
・Proprietary confocal method enables rapid acquisition of 3D images
・Second camera allows simultaneous dual-wavelength imaging
・Optional ultra-high-speed mode up to 100 fps is ideal for fast applications such as cardiomyocyte beating and calcium signaling
High Resolution
・High-NA water-immersion objectives provide bright, deep imaging
・Equipped with a high–quantum-efficiency sCMOS camera
・Uniformizer enables even illumination across the entire field of view
Live-Cell Imaging
・Industry-leading incubator performance ensures stable long-term live-cell imaging
・Supports incubation for up to 7 days
・Confocal scanner unit CSU enables imaging with low photobleaching and low phototoxicity
Automation
・Automatically scans the entire sample and captures images that meet predefined conditions
・Supports external system integration through an API
・Outputs data in widely compatible formats such as OME-TIFF and OMERO
Details
High Throughput
Throughput comparison when using the 2nd camera option.
Detects two colors at the same time, so acquisition time is reduced.
In addition, if confocal performance is unnecessary such as during low magnification imaging, widefield imaging can further shorten the exposure time.
Fast Time Lapse Function
Capable of capturing up to 100 images per second (100 fps) simultaneously with two wavelengths.
It makes possible to capture fast dynamics such as myocardial pulsation, calcium firing, and intra-axonal signal transduction can be captured, which have been difficult to capture until now.
iPSC-derived cardiomyocytes in culture on a gelatin fiber substrate for cell culture (Genocell® Myocardial Evaluation Plate, Japan Wool Co.) Bright field image (left), stained with calcium-sensitive fluorescent dye (right).
Calcium signal waveform fluctuating periodically in response to myocardial pulsation (upper panel). High-speed imaging at 100 frames per second allows the fast-rising portions of the waveform to be captured at a sufficient sampling frequency. High-speed imaging at 100 frames per second allows the fast-rising portions of the waveform to be captured at a sufficient sampling frequency.
High Resolution
- Water immersion lens captures clear images with a high SNR.
- In addition to fine observation via high magnification, it can accurately analyze images even with FOVs smaller than before due to the high-NA lens.
Our high-NA 20x water immersion lens developed in-house allows for image resolutions higher than a 20x dry lens. You can use 40x and 60x for efficient image analysis.
Imaging example for a spheroid
20x WI lens allows for deeper imaging with high SNR.
CE- Bright Field
Creates two types of contrast-enhanced images from bright-field images acquired at multiple Z positions. One is an epifluorescence-like image (Fluor Type), which is useful for recognition of cell nuclei. The other is a normal DPC-like image (Phase Type), which is useful for recognizing cytoplasm.
By enhancing the contours of the brightfield image, changes in the state of the cell can be accurately identified. It is also effective when it is difficult to recognize regions in brightfield image analysis.
Live-Cell Imaging
- The CSU confocal scanner unit's low phototoxicity and high-precision temperature and position control technology enable time-lapse imaging under gentle, stable cell culture conditions.
- The automatic water supply mechanism enables longer imaging sessions
CQ3000 achieves culturing and imaging while maintaining the same environment as a conventional CO2 incubator.
It’s not only environmentally friendly, but it also minimizes the impact on cells.
Automation
Target Search
It can scan the entire well at low magnification, detect the position of the object based on the analysis results, and acquire images at high magnification. This makes it possible to image samples where it is not possible to know where the object is located in the well, or to image and analyze only the cells that match the conditions from among a large number of cells.
- High magnification imaging only in the field where the target is detected, greatly reducing the amount of data and throughput.
- Reduce unnecessary images and tiling by imaging at the center of the target.
- Automation of the two imaging processes reduces human intervention time and human error.
Analysis Software
- Select analysis menus from a wide range of preset options
- Display calculated numerical data in various graph formats
- Supports linking graphs to images
- Supports tile display and analysis functions
- Added area recognition method
Machine learning
Software learns the features of the sample objects collected by users.
3D analysis
Label-free analysis
Wide-field*1 function is a powerful tool to analyze unstained bright field samples.
*1 Can be acquired when Uniformizer option is selected.
Specifications
| Fluorescence Excitation Light Source | 405 nm, 488 nm, 561 nm, 640 nm solid-state lasers |
|---|---|
| Objective Lens | Max. 6 lenses (Included max. 2 water immersion lenses) Dry : 2x, 4x, 10x, 20x, 40x , 60x Long working distance : 20x, 40x Water immersion: 20x, 40x, 60x |
| Camera | High-sensitivity sCMOS Max. 2 units, simultaneous excitation of 2 wavelengths Number of effective pixels : 2000 x 2000 pixels Field of view size: 13.0 x 13.0 mm |
| Autofocus | Laser autofocus, Image-based autofocus |
| Flat-top beam shaper（Option） | Uniformizer |
| Stage incubator | Temperature control range 35 ~ 39 °C Settable temperature resolution : 0.1 °C Time stability : ±0.2 °C Spatial stability : ±1 °C (at an ambient temperature of 21 ~ 25 °C) Humidity holding Automatic water supply function for incubator |
| Fast time-lapse (Option) | Max.100fps, Simultaneous dual wavelength excitation imaging |
| Sample search function (Option) |
Target Search |
| Analysis software(CellPathfinder) | Granule analysis, Neurite analysis, Nuclear morphology analysis, Nuclear translocation analysis, Membrane translocation analysis, Machine learning, Label-free analysis, 3D analysis, Texture analysis, Deep Learning, etc. |
| Other features | Self-diagnosis function, CQ Analysis with 3D Viewer |
| Software (Option) | High-content analysis software CellPathfinder |
Remote Diagnosis Service
Equipment status can be remotely diagnosed. This service helps ensure long-term stable operation of the equipment.
Regular diagnosis can predict and detect problems early, reducing equipment downtime.
*Service available only in the USA, Japan, the UK, Switzerland, EU countries.
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YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.
In this webinar, Professor Jonny Sexton discusses a pipeline, developed in the Sexton lab, for the quantitative high-throughput image-based screening of SARS-CoV-2 infection to identify potential antiviral mechanisms and allow selection of appropriate drug combinations to treat COVID-19. This webinar presents evidence that morphological profiling can robustly identify new potential therapeutics against SARS-CoV-2 infection as well as drugs that potentially worsen COVID-19 outcomes.
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Persbericht | Oplossingen en Producten January 31, 2024 Yokogawa introduceert het CellVoyager CQ3000-systeem voor analyse van hoge concentraties
Voor een grotere efficiëntie in O&O op het gebied van geneesmiddelenontdekking en regeneratieve geneeskunde, en de snelle commercialisering van nieuwe geneesmiddelen
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