CSU-W1 Confocal Scanner Unit

Widest field of view in the industry providing 4X wider FOV than conventional scanners.

The CSU-W1 Confocal Scanner Unit is our widest field of view confocal, providing the clearest image resolution of our imaging systems. The system features switching mechanisms to enable fully automated experiments and a newly designed disk unit to improve image clarity of thick samples.

  • Wide and clear
  • Near Infrared (NIR) Port: Up to 785nm
  • 3 configurations: 1-camera model, 2-camera model, split-view model
  • New bright field path (Standard)
  • Selectable pinhole size:  25 pinhole disk, 50 pinhole disk or double disk
  • External light path
  • 10-position filter wheel (1 Camera model, 2 Camera model)
  • Fully automate experiments with the motorized switching mechanism

Wide

Widest FOV confocal! Provides 4 times wider FOV than the conventional model.

Widest FOV confocal


Clear

Newly designed disk unit offers much improved image quality. Due to significantly reduced pinhole crosstalk, CSU-W1 enables clear observation much deeper into thick samples.

Conventional model

XY MIP

XZ Slice

XY MIP:Conventional XZ Slice:Conventional

CSU-W1

XY MIP

XZ Slice

XY MIP:CSU-w1 XZ Slice:CSU-w1

Mouse ES cell colony
Fluorescent probe:  H2B-EGFP(Excitation:488nm) mCherry-MBD-NLS(Excitation: 561nm)
Objective lens:  60x silicone
Z-sections/stack:  100um(0.4m/251slices)
By courtesy of Jun Ueda, Ph.D. and Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University

Thin sample:Conventional,Thick sample:Conventional,Thick sample:CSU-W1

Flexible

Flexibly selectable functions to meet versatile applications.

High confocality pinhole (Optional Component)

In addition to our conventional 50um pinhole size, 25m pinhole size with higher confocality is available.
You can select either one or the both pinhole size, with easy-to-use motorized disk exchange mechanism.

Disk unit

New bright field through path (Standard)

New mechanism to move the disks out of the light path allows much easier projection of confocal and non-confocal images such as phase contrast.

Simultaneous dual color imaging mechanisms
(T2 and T3 Models)

CSU-W1 offers single camera split-view model, in addition to the dual camera model which are much improved from those for the CSU-X1. Thanks to the wide FOV, even the split-view offers 2 times wider image area than with older model. By using various dichroic mirrors, it is possible to select various dye-combinations for dual-color imaging*1 with both the two camera model and split-view model.

CSU-W1 offers selection from a total of three basic configurations, two pinhole sizes, options for near infrared observation and an external light path which is useful for versatile applications such as photo bleaching, while bright field light path is now a standard feature. All switching mechanisms in the CSU-W1 are fully motorized and thus ready for automated experiments.

Basic Configurations

CSU-W1 provides a total of three basic configurations for multi- color imaging; 1) Sequential imaging with one camera and a filter wheel, 2) Simultaneous two-color imaging with two cameras, and 3) Split-view two color imaging with one camera shared by 2 optical paths. All features are upgradable after installation.

Basic Configurations

Filter

Filter

Option

SoRa disk

Optical resolution has been improved by approximately 1.4x using a super-resolution technique based on spinning-disk confocal technology. Furthermore, a final resolution approximately twice that of the optical limit is realized through deconvolution.
Upgrading from the CSU-W1:CSU-W1 SoRa

Near Infrared (NIR) Port

NIR port provides up to 785nm excitation capability to allow less-invasive deep imaging. The NIR laser is introduced via a dedicated optical fiber in the same way as visible lasers. It is possible to combine NIR and visible lasers within the CSU-W1 unit to allow simultaneous excitation.

External light path

External light path provides the direct path bypassing the disk s to microscope. Versatile applications such as photo activation are available by introducing an external light scanner through this port.

Lens switcher

Newly designed motorized lens switcher between 2 relay lenses i s useful for fitting CSU-W1 image size with various camera types, and also for easy magnification change without exchanging objective lenses.

Variable aperture

Variable aperture to change laser illumination area, and thus the imaging area by the CSU-W1, is useful to minimize laser damages in the specimen.

Selectable option

Option 1 Camera model 2 Camera model Split-view model
NIR port ×
External light path ×
Variable aperture × N/A
Camera port lens Selectable from
0.83x, 1x
Selectable from
0.83x, 1x
(1 Camera)
Selectable from
0.83x, 1x
(2 Camera)
Selectable from
0.83x, 1x
Additional lens
to lens switcher
Selectable from 0.83x, 1x, 2x N/A

2 Camera model, 1 Camera model

2 Camera model, 1 Camera model

 

Split-view model

Split-view model

*1  2 Camera model  *2  2 Camera model and Split-view model
*3  1 Camera model and 2 Camera model  *4 Under development

External Dimensions

External dimensions

Microscope-setup
 

Zeiss Axio Observer
Zeiss Axio Observer

Nikon ECLIPSE Ti
Nikon ECLIPSE Ti

Olympus IX83
Olympus IX83

Leica DMi8
Leica DMi8

General Specifications
Model 1 camera model (T1) 2 camera model (T2) Split-view model(T3)
Confocal scanning method Microlens-enhanced Nipkow disk scanning
Spinning speed 1,500rpm - 4,000rpm Max200fps
External synchronization Scan-speed synchronization through pulse signals Input/output : TTL level 300Hz up to 800Hz
Disk unit Selectable up to 2 disks from 50um
(for high magnification)
and 25um(for low magnification)
Motorized switching
Bright field Motorized exchange between confocal and brightfield
Effective FOV 17×16mm
Excitation wavelength 405nm-785nm
Laser introduction Yokogawa's standard fiber*1 VIS Laser port (405-647nm)
【Option】NIR Laser port (685-785nm)
Observation wavelength 420nm-850nm
Dichroic mirror switching Motorized 3CH (Dichroic mirror block can be exchanged)
Emission filter wheel 10-position filter wheel
Filter sizeφ25mm
Switching speed*2 : 100msec max.(Standard mode) 40msec max.(High speed mode)
6-position filter wheel
Filter sizeφ25mm
Switching speed*2 : 100msec max.
External control RS-232C (CSU-X1 command upper compatible)
Microscope mount Yokogawa original
Camera adaptor C mount 1x (Variable magnification: 0.83x )
Light introduction port 【Option】Photo breach etc
Operating environment 15-30oC、20-75%RH No condensation
Power consumption Input : 100-240 VAC ±10% 50-60Hz 250VAmax
External dimension Main unit 327.1(W)x
251.5(D)x
475.1(H)mm
471.6(W)x
251.5(D)x
475.1(H)mm
420.8(W)x
251.5(D)x
373.6(H)mm
Power unit 225.4(W)x 151.9 (D)x 378.3(H)mm
Weight Main unit 17kg 20.5kg 18kg
Power unit 4.5kg
Attachable microscope Olympus IX series, Nikon ECLIPSE Ti , Zeiss Axio Observer, Leica DMI series*3

*1 Each CSU-W1 head is optimized with its fiber at factory. Please inquire about fiber exchange if necessary.
*2 Adjacent position.
*3 Some microscopes/options could limit the FOV of CSU-W1 or connection with CSU-W1, please inquire.

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Intelligent Imaging Innovations

Intelligent Imaging Innovations  
(product name: Marianas and VIVO)


Nikon

Nikon Instruments Inc.  
(North and South America)

Nikon Instruments Europe B.V.  
(Europe)

Nikon Corporation  
(Asia except for Japan and Oceania)
 




 

Notas de Aplicación
Overview:

Wide and Clear
Confocal Scanner Unit

Overview:

Time-lapse imaging : Early stage mouse embryo

Following the injection of mouse embryos with mRNA, nearly 25,000 multicolor and multilayer confocal images of the embryos were acquired over 48 hour period as they developed to the blastocysts stage.Thereafter, they were transferred to a recipient mouse that gave birth to healthy pups, each of which developed normally and had full reproductive capability.This is firm evidence that long-term, multi-dimensional confocal imaging with CSU causes no harm to a delicate specimen such as an early stage embryo.

Early stage mouse embryo

Time lapse (MIP)  |  Full size movie Play

Measurement condition
Z-sections/
stack
100um
(1um/101slices)
Fluorescent probe 488nm:
H2B-EGFP
561nm:
mCherry-MBD-NLS
Pinhole 50um
Objective lens 60x silicone
Total time 48 hours
(Interval:10mins)
 Early stage mouse embryo

Excerpts from Time lapse (MIP of chromosome)

Data:  Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University

Overview:

List of Selected Publications : CSU-W1

Overview:

The neuronal network is a computing system that transforms input to output. This computation involves complex nonlinear processes through polysynaptic feedforward and feedback microcircuitry, and thus cannot be addressed either with isolated neuron responses or averaged multineuronal responses. Functional multineuron calcium imaging (fMCI) is promising to solve this problem.
The fMCI is a large-scale recording technique that simultaneously monitors the firing activity of more than a thousand neurons through their somatic Ca2+ signals.
Because of several advantages, including i) simultaneous recording from numerous neurons, ii) single-cell resolution, iii) identifiable location of recorded neurons, and iv) detection of non-active neurons during the observation period, fMCI attracts attention as a new-generation large-scale recording method.
In vitro fMCI is made more sophisticated by using multipoint ilumination and scanning with the CSU in combination with low-intensity lasers and an EM-CCD (electron-multiplying charge-coupled device) camera.
This CSU system allows to achieve ultra-high-speed and high-resolution fMCI in hippocampal slices; the Ca2+ fluorescent intensity of a large number of neurons can be monitored at the speed of up to 2,000 frames per second. This is one of the applications that make best use of the high-speed performance of the CSU Confocal Scanner Unit.

fMCI

Data: Yuji Ikegaya, PhD, Associate Professor at University of Tokyo Graduate School of Pharmaceutical Sciences.

 


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Overview:

To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

Overview:

In the fertilization and early embryonic development process, various events are spatiotemporally controlled, and many events are connected in the cause-effect relations toward the final goal of ontogenesis. To understand the mechanism of this process, conventional experimental techniques by fixing and destruction of the cells have limitations. If this process can be observed over time and the development process can be continued after the observation, it will open a new era in the Genetics research. A mammalian developmental biology researcher, Dr. Kazuo Yamagata, established such technique by using the CSU system.
He successfully imaged mouse embryos over a long period of time, from the post-fertilization through to the blastocyst stage, to acquire approximately 60,000 of 3D confocal images. Thereafter, the embryos were transferred to a recipient mouse, and the pups were born all normally, grew healthy, and were capable of reproduction; a firm evidence that this early embryo imaging technique does not adversely affect the process of full-term development. The high speed image acquisition and extremely low excitation light unique for the CSU system enabled greatly reduced phototoxicity and realized intensive but damage-free long time observation. Only by using this technology which does no harm on the embryonic development, it is possible to “utilize the same embryo after intensive analysis by imaging” , and thus to investigate cause- and-effect relationship of various early stage phenomena and their influence on the development.

Experimental flow
Movie example

Figure : The long-time, multi-dimensional live cell imaging on early stage embryos does not affect the process of ontogenesis.
(a) Experimental flow
(b) Movie example: Images were acquired at 7.5-minute intervals over approximately 70 hours.
      This figure shows extracted images at 2-hour intervals.
    Each image is the maximum intensity projection of a total of 51 images in the Z-axis direction.
    Green:Spindle (EGFP-α‒tubulin), Red:Nucleus (H2B-mRFP1)
 

Experimental conditions
Total time 70 hours
Interval 7.5 min/stack
Z-axis slices 51 sections (at 2μm intervals)
Channel 3(DIC, EGFP, mRFP1)
Position 6(Total 72 embryos)
Laser power(Measured at objective lens) 488nm (0.281 mW), 561nm (0.225 mW)


Data: Kazuo Yamagata, PhD., Laboratory for Genomic Reprogramming,Center for Developmental Biology, Riken


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Overview:

Real time live cell imaging of mitochondria

Image provided by Dr. Kaoru Kato, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)


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Overview:

In recent years, obese adipose tissue is attracting attention as an “active metabolic organ” that causes various diseases. Especially, visceral obesity and inflammation play a central role in metabolic syndrome. It was found that visceral obesity caused remodeling of adipose tissues based on chronic inflammation, and insulin resistance was occurred, which eventually leads to development of arteriosclerosis lesion, and cause new blood vessel events.
To elucidate the molecular mechanisms of pathological conditions consisted by the complicated and multi-cellular abnormal interactions in remodeling tissues, an “in vivo molecular imaging” based on the CSU system was developed.
By using this technique, it becomes possible to precisely evaluate the three-dimensional changes in the structures in living tissue, and the multi-cellular dynamics in vivo with high time and spatial resolutions.

 

Images of the remodeling of adipose tissue in live animals

Figure 1: Images of the remodeling of adipose tissue in live animals
a: Conventional adipose tissue specimen (lean, db/+ mouse)
b & c: Images of a white adipose tissue of an 8-week-old thin mouse (lean, db/+)
d: Adipose tissue of an 8-week-old obese animal (obese, db/db)
 

 

An example of real-time multi-color movie of microcirculation in mouse

Figure 2: An example of real-time multi-color movie of microcirculation in mouse, which clearly shows dynamic movement and interactions among leucocytes, platelets, macrophages and endothelium.

 

Application of “ in vivo molecular imaging” on various organs

Figure 3: Application of “ in vivo molecular imaging” on various organs
(Blood flow images of a: Skeletal muscle, b: Liver, c & d: Kidney glomeruli)

Data: Satoshi Nishimura M.D., Ph.D www.invivoimaging.net
Dept. of Cardiovascular Medicine, Translational Systems Biology and Medicine Initiative,
The University of Tokyo & PRESTO, Japan Science and Technology Agency


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Reporte Técnico de Yokogawa
The World as Seen from Cells
(rd-te-r06002-001)
2.2 MB

Descripciones del Producto

    Overview:

    YOKOGAWA proprietary Spinning Disk technology enables fast real-time confocal imaging for applications such as high-speed 3D and long-term live cell imaging. These quantifiable imaging analysis are essential tools for modern precision drug discovery.
     

    Overview:

    Over past 20 years, YOKOGAWA proprietary Spinning Disk Confocal technology has been widely used as an indispensable imaging tool among top researchers. The technology enables faster live-cell observation with clearer and less photo-bleaching imaging.

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