CSU22 Stopgezet

Different from conventional confocal systems , CSU uses CCD cameras to capture confocal images. Taking advantage of rapid advance in CCD camera capability, it is more and more possible to acquire high-resolution images at high speed. Especially, significantly reduced photo bleaching and photo toxicity with CSU makes it possible to image delicate changes in living cells, such as cell division or embryonic differentiation at length.

Observation of cell division in a drosophila embryo

Time-lapse images of cell division : ( centromere : labeled with EGFP )

By courtesy of Dr. J.Sholey , Dr. D.Sharp : University of California , Davis

High-speed scanning up to 1,000 frames/sec makes it possible to record reactions at milliseconds level

High-speed imaging using an ultrafast CCD camera with GenlV image intensifier

Time elapsed since electrical stimulation :
          16ms                         20ms                          24ms                              28ms
 

Post-electrostimulation changes in Ca2+ in mouse ventricular cardiac muscle cell labeled with Fluo-3 , images taken at 4 ms intervals

By courtesy of Dr. Hideyuki Ishida , Dept. of Physiology , School of Medicine , Tokai University (in Japanese)

3D reconstruction data of living cells can be acquired at ease from stacks of sharp confocal images captured by a high-resolution CCD camera.

     3D-constructed images of drosophila's salivary chromosome ( labeled with PI ) (×60 oil )

By courtesy of Dr. Yoshihiro Akimoto , Second Dept. of Anatomy
,School of Medicine , Kyorin University

In synchronization with fast objective lens movement using piezo-actuator and a high-speed camera, CSU22 enables you to perform high-speed 3D scanning of 30 or more cross-sectional images per second .

Example of Application

3D Reconstruction of Moving Objects

3D tracking of the behavior of C.elegans expressing EGFP-fused pharyngeal protein (3D images were reconstructed after acquiring each 30 cross section images per second)

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Real time 3D imaging of signal transduction

Single section confocal images

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Projection ( overlay ) of 30 cross-sectional images per second

High-speed 3D scanning of glutamic acid-stimulated Ca²⁺ reactions in cerebral cortical nerve cells labeled with Fluo-3

By courtesy of Dr. Atsuo Fukuda , Dept. of Physiology ,Hamamatsu University School of Medicine.

CSU can generate real color confocal images thus can utilize a highly sensitive 3CCD color camera, which enables simultaneous multi-wavelength imaging as fast as at video rate.

Combining the CSU22 with a high-sensitivity high-resolution 3CCD color camera makes simultaneous multicolor observation without the need for filter replacement a reality .

Observation of the process of Golgi aparatus formation in budding yeast

Real color imaging of formation of Golgi aparatus in budding yeast ( Saccharomyces cerevisiae). It is possible to clearly recognize cis-cisterna ( EGFP ) and trans-cistema ( RFP ) behave independently while dynamically interacting with each other.

By courtesy of Dr.Akihiko Nakano , Molecular Membrane Biology Lab., Discovery Research Intitute , Riken & Prof .
Graduate School of Science , University of Tokyo.

By using a beam splitter in combination with a CCD camera, simultaneous dual color imaging as fast as millisecond level can be achieved.

Imaging of Microcirculation in live small animals :
Time lapse imaging of intravascular thrombus formation after laser irradiation

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Dynamic Movement of each individual platelet (spot indicated by arrow in the "4 sec" image above) can be clearly recorded at video rate :platelets (green) , fibrinogens (red).

By courtesy of Dr. Hideo Mogami, Dept. of Physiology, Hamamatsu University School of Medicine

Reduced fluorescence photo bleaching and high-speed imaging with CSU22 is best for confocal FRET observation in real time!

Real time , real color imaging of the initial stage of histamine-stimulated Ca2+ concentration in HeLa cell cytosol expressing Cameleon ( YC3.60 )

Video-rate FRET images : Excerpts at 264ms interval
Real-color images

Ratio images ( Pseudo-colored )

By courtesy of Dr. Atsushi Miyawaki, Advanced Technology Group, Brain Science Institute, Riken and Dr. Takeharu Nagai, Lab. for Nanosystems Physiology, Research Institute for Electronic Science, Hokkaido University.

Upright microscopes

Inverted microscopes

Note:The CSU22 can be mounted on microscopes from different manufacturers

General Specifications

Note : Use of Infinity corrected microscope with high NA objective lenses (i.e.,Plan Apo) are recommended.
General specifications are subject to change without prior notice. The standard model does not include any peripheral components such as a microscope, a laser unit, optical fiber, camera, image monitor, or image processing unit. For more information, contact the office indicated below.