Visualizing the complex spatiotemporal dynamics of human stem cells as they proliferate and make cell fate decisions is key to improve our understanding of how to robustly engineer differentiated tissues for therapeutic applications.
In this webinar, Dr. Rafael Carazo Salas will describe multicolor, multiday high-content microscopy pipelines that his group has recently developed to visualize the dynamical cell fate changes of human Pluripotent Stem Cells (hPSCs). In particular, he will describe the integrated experimental and computational approaches that his group has established, including novel “live” reporters of cell fate and multi-reporter hPSC lines generated by CRISPR/Cas9 allowing multiplexed monitoring of cell proliferation and fate dynamics, and exemplify the biological discoveries they are enabling.
Key Topics Include:
- Visualizing how human Pluripotent Stem Cells (hPSCs) proliferate and undergo early differentiation in vitro, by high content microscopy
- Learning about experimental and computational pipelines that enable monitoring single-cell fate dynamics
- Learning about novel “live” reporters of hPSC cell fate
Zugehörige Produkte & Lösungen
-
Benchtop-System
Das CQ1 bietet Konfokalbilder und erweitertes Live-Cell-Imaging der höchsten Qualität in einem raumsparenden Benchtop-Design.
-
High-Content-Screening
CellVoyager CV8000 ist das fortschrittlichste High-Content-Screening-System. Der optimierte eingebaute Inkubator gewährleistet bessere Umgebungsbedingungen für Ihre Langzeit-Live-Cell-Experimente. Mit seiner Ausbaufähigkeit zu bis zu vier Kameras, fünf Lasern und einem optionalen Pipettier-Roboter erlaubt das System die Durchführung komplexer Assays und High-Content-Screening-Experimente.
-
Life Science
Die High-Content-Analyse-Systeme (HCA) und Dual-Spinning-Disk-Technologien von Yokogawa ermöglichen hochauflösendes Hochgeschwindigkeits-Live-Cell-Imaging für die Spitzenforschung rund um den Globus.