CV7000S (DISCONTINUED)

Notice: This product was discontinued on Dec 29, 2017. See this replacement product:

Cell Voyager 7000S (CV7000S) is a high content screening system with our original confocal scanner unit, a live cell stage incubator and a build-in liquid handler which enable long term live cell imaging and rapid kinetic analysis. CV7000S is the most powerful microscopic system for drug discovery based on phenotypic screening as well as life science researches.

High throughput

  • New wide-view multi-beam confocal scanner unit provides ultimate high-speed image acquisition
  • At less than 60 sec., imaging acquisition is complete for a 96 wellplate*1
  • By only single-shot, image of whole-well in a 384 wellplate is acquired*2
  • Microplates of various manufactures are selectable from 6 to 1536 wells

Live cell is observation

  • Fast phenomenon in live cell is observable with the maximum image acquisition rate of 38fps*3
  • Proprietary multi-beam confocal scanning provides minimal photo-bleaching and photo-toxicity
  • Optional stage incubator provides long-term time lapse imaging over 3 days*4
  • By dispensing reagents under image acquisition, kinetics of live cell can be observed and analyzed
  • It is possible to dispense different reagents in different time into one well

Flexible software

  • Flexible customization provides optimal algorithms for your application
  • Interactive GUI provides easy setting of measurement procedures and stress-free operation of imaging/data analysis
  • The parallel high speed processing and multi batch analysis are available by multi CPU

Versatile functions and expandability

  • Up to a total of 6 objective lens are available(4x-60x), including water immersion lenses with automated water supply
  • Plate loaders interface provides flexible connections with external robot systems of various manufacturers
  • Bar-code reader is optionally available for easy handling of wellplate information
  • Analysis starts automatically just after image acquisition
  • Slide glasses are usable as samples
  • Fluorescent cross-talk can be reduced by improved image date processing

*1 One image per each well
*2 Using 4x objective lens
*3 At 3x3 binning of camera pixels
*4 Depends on condition of experiment

Suitable for a wide range of applications

  • Calcium flux
  • Cell cycle
  • Spheroid growth
  • Cell motility
  • GPCR internalization
  • Neurite outgrowth
  • Mitochondria morphology
  • Differentiation of ES cells
  • Translocation from cytoplasm and nucleus of target proteins
  • Translocation from cytoplasm to cell membrane
  • Micronucleus assay
  • Apoptosis
  • Colony population

Selected publications

  • Cell-to-cell propagation of infectious cytosolic protein aggregates. Hofmann JP et al., Proc Natl Acad Sci U S A. 2013 ;110: 5951-6.
  • Image-based transcriptomics in thousands of single human cells at single-molecule resolution. Battich N. et al., Nat Methods. 2013 ;10: 1127-3
  • Reversible centriole depletion with an inhibitor of Polo-like kinase 4. Wong YL. et al., Science, 2015; 348: 1155-60.
  • Cell-intrinsic adaptation of lipid composition to local crowding drives social behaviour. Frechin M. et al., Nature, 2015 ; 523: 88-91.  

Academic customers

  • DZNE-Bonn
  • DZNE-Tubingen
  • University of Zurich
  • Palacky University
  • University of Dundee
  • Max Planck Institute
  • Boston Children’s Hospital/ Harvard Medical School
  • Ludwig Cancer Research Institute/ University of California San Diego

Easy setup of multiple complex measurement parameters

 

 

Cell counting

Cell Counting & Cell Searching

  • Automatically increases number of imaging points until number of cells imaged reaches to target value.
  • Automatically searches appropriate imaging position in a well to acquire image containing target number of cells.

Cell counting

Cell searching

Auto Analysis Start

Automatically transfer the measurement data to analysis WS after image acquisition.
Post-processing includes "Copy", "Move", "Image Correction" and “Starting Auto-analysis” of measurement data.

Wide-view imaging

Wide-view optical scanner has 4x wider image area than our conventional model and provides single-shot image acquisition of whole-well in a 384 wellplate using 4x lens (4 shots using 10x lens).

Comparison of new and conventional image areas

Comparison of new and coventional image areas

Example of single-shot images of entire 384 wellplate

Example of single-shot images of entire 384 wellplate

Wide variety of imaging/display modes

Various imaging/display modes are available to select the best for your application.

Example of display modes

Slice images
Slice images

MIP
MIP
 

Example of imaging modes 

Confocal

Confocal

Epi-fluorescence 
Epi-fluorescence

MIP
MIP

Reduction of fluorescent cross-talk

Fluorescent cross-talk is an artifact caused by fluorescent signal that is superimposed on other fluorescent color(s) in simultaneous multi-wavelength fluorescent image acquisition.
This artifact can be reduced by image processing.

Example images of fluroescent cross-talk and resultant image of cross-talk reduction

cross-talk reduction

This software allows you to flexibly customize analytical algorithms depending on your application.

Analytical protocols

Ready-to-use standard protocols are available for universal basic analyses. New appropriate protocols can also be built for your application.

Analysis protocol Explanation
Granularity Granule recognition in cells
Neurite Outgrowth Recognition of nerve cell projections
Nuclear Morphology Recognition of morphological changes of nuclei
Nuclear Translocation Recognition of objects migrating between the cytoplasm and nucleus
Plasma Membrane Translocation Recognition of objects migrating between the cytoplasm and cell membrane
New Creation of new protocol

Object recognition

Basic and advanced modes are available according to application. In the basic mode, pre-defined parameters such as signal intensity threshold or size can be used to recognize objects. In the advanced mode, appropriate algorithms for object recognition can also be used.
(Preprocessing/Binarization/BinaryTransform/Labeling/LogicalOperating)

Object recognition

Graph of analytical result

Each object is linked to the graph, showing a more detailed analysis of results is possible.

Graph of analytical result

Object link and grouping

This function links multiple different objects of the same cell in different images.

Object link and grooping

Parallel high speed processing and multi batch processing

Using this function, multiple images of the same measurement can all be processed automatically in the same analytical protocol (recognition algorithms and/or analysis parameters). Moreover, by setting multiple different batch processes, different analyzes of many images can be processed sequentially.

Batch processing

Tiled image analysis

Using tile function, all images of entire well can be reconstructed and analyzed as single image.

Tiled analysis

Hardware configuration

We can offer a system tailored to various customers' needs with most versatile options

Dispenser unit*1

Dispenser unit
  • Single head
  • Disposable-tip(For wellplates of 96 or 384 wells)
  • Numerous dispensing functions
  • Capable of dispensing different reagents in different time into one well(Multi dispensing)

Stage incubator*1

Stage incubator
  • Temperature and CO2 concentration control, humidifier
  • Improved environmental control*3
  • Time-lapse imaging over 3 days*4

High-speed, high-precision XY stage

  • Fastest measurement speed in the class (4 minutes for 384 wellplate)
  • High-precision positioning

Objective lens*1

Dry 4x, 10x, 20x, 40x
Water immersion 60x
Long working distance 20x
Phase contrast*2 10x, 20x
  • Automatically switchable
  • Up to six lenses
  • Automated water supply unit for water immersion lens

Highly sensitive, wide-view with high-resolution, sCMOS camera

  • 4x wider image area*5
  • Capable of simultaneous multi-color imaging
  • Clearer image with cooled sCMOS camera

*1 Option
*2 Only for 6 and 24 wellplates
*3 CO2 concentration in stage is calibrated before shipping
*4 Depends on condition of experiment
*5 Compare with our conventional model

Hardware configuration

Hardware configuration

Plate loaders / lifter

  • One-click operation for sample setting and dispensing
  • Capable of connecting with external robot systems
  • In combination with external robot systems*1, you can load the wellplates directly from the incubator for imaging and analysis*2

Plate loaders,lifter

Bar-code reader

Bar-code reader
  • Useful for easy handling of wellplate information
  • Up to two sets(For assay/source plates)

*1 Option
*2 Please contact dealer about the specification

Optical technology

optical technology

Confocal

Confocal

Epi-fluorescence

Epi-fluorescence

Phase-contrast

Phase-contrast*1*2

Wide-view confocal scanner unit

Microlens-enhanced dual Nipkow disk technology significantly reduces photobleaching and phototoxicity in live cell imaging

Principles of the Microlens-enhanced Nipkow Disk Scanning Technology

A Nipkow spinning disk containing about 20,000 pinholes and a subsidiary spinning disk containing the same number of microlenses to focus excitation laser light into each corresponding pinhole are mechanically fixed on a motor, and very rapidly rotated. As a result, high-speed raster scan of the excitation lights on the specimen can be achieved.The pinhole and microlenses are arranged on each disk in our proprietary design to optimize raster scan.
Multi-beam scanning not only increases scanning speed, but also results in significantly lower photobleaching and phototoxicity, because multi-beam excitation needs only low level of laser power on the specimen to fully excite fluorescence.
CV7000 uses wide-view Nipkow disk model which is capable of scanning 4 times wider imaging area with higher resolution and thus capable of ultrafast and high-resolution screening.

Microlens-enhanced Nipkow Disk Scanning

Confocal scanner unit CSU-W1

*1 Option
*2 Only for 6 and 24 wellplates

High-throughput Cytological Discovery System

Model CV7000
Well plate format 6, 24, 96, 384, 1536 well
Observation mode Confocal mode
(simultaneous imaging of max.3 colors),
Epi-fluorescence mode
(simultaneous imaging of max.3 colors)
【Option】
Brightfield mode, Phase-contrast mode
(For 6, 24 well plate)
Output data format Image data: 16bit TIFF, PNG
Numerical data: CSV, Original format
Excitation wavelength 405/488/561/640nm, all solid laser
【Option】365nmLED
White light illumination 【Option】100W halogen lamp
Autofocus Laser-based mode, Image-based mode
Objective lens Max. 6 lenses are available : Automatically switchable
Dry: 4x, 10x, 20x, 40x
Water immersion: 60x
Long working distance: 20x
Phase-contrast: 10x, 20x (For 6, 24 well plate)
Confocal unit Microlens-enhanced wide-view dual Nipkow disk confocal scanner
Camera sCMOS camera
Effective no. of pixels:2560×2160, Cell size: 6.5um, Cooling:-20℃
Max.3 cameras
Stage incubator 【Option】 Capable of live cell imaging.
Temperature range: 35 up to 40ºC, CO2 supply box(CO2:5%, humidifier)
Dispenser 【Option】Disposable tip type
(For 96, 384 well plate)
Bar-code reader 【Option】Target codes: 1 up to 2 dimension
Workstation Dual-monitor workstation for system control
Dual-monitor workstation for data analysis
Analysis software Granularity, Neurite Outgrowth, Nuclear Morphology,Nuclear Translocation, Plasma Membrane Translocation
Operating environment 15 up to 30ºC, 20 up to 70%RH (No condensation)
Power supply Measurement unit: Single-phase 200VAC, 2KVAmax
Workstation for system control : 100VAC, 1.6KVAmax, Workstation for data analysis: 100VAC, 1.4KVAmax
External dimension Measurement unit: 1496W×1160D×1650H mm,
Workstation for system control :
1000W×700D×1200H mm
Weight Measurement unit: 650Kg,
Workstation for system control : 50Kg
Application Note
Overview:

Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System

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