Image-based phenotypic screening relies on extraction of multivariate information from cells cultured in a large number of screened conditions. In this webinar, we explore the application of complex and biologically relevant model systems for drug screening, such as small intestinal organoids.
We describe several processes used to infer regulatory genetic interactions and identify genes that regulate cell-fate transitions and maintain the balance between regeneration and homeostasis, unravelling previously unknown roles for several pathways, among them retinoic acid signalling.
We then characterise a crucial role for retinoic acid nuclear receptors in controlling exit from the regenerative state and driving enterocyte differentiation.
Finally, by combining quantitative imaging with RNA sequencing, we show the role of endogenous retinoic acid metabolism in initiating transcriptional programmes that guide the cell-fate transitions of intestinal epithelium and identify an inhibitor of the retinoid X receptor that improves intestinal regeneration in vivo.
Learning outcomes of this webinar:
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