Image-based phenotypic screening relies on extraction of multivariate information from cells cultured in a large number of screened conditions. In this webinar, we explore the application of complex and biologically relevant model systems for drug screening, such as small intestinal organoids.
We describe several processes used to infer regulatory genetic interactions and identify genes that regulate cell-fate transitions and maintain the balance between regeneration and homeostasis, unravelling previously unknown roles for several pathways, among them retinoic acid signalling.
We then characterise a crucial role for retinoic acid nuclear receptors in controlling exit from the regenerative state and driving enterocyte differentiation.
Finally, by combining quantitative imaging with RNA sequencing, we show the role of endogenous retinoic acid metabolism in initiating transcriptional programmes that guide the cell-fate transitions of intestinal epithelium and identify an inhibitor of the retinoid X receptor that improves intestinal regeneration in vivo.
Learning outcomes of this webinar:
- Learn how to upscale, streamline and automate intestinal organoid handling
- Learn how to image in complex three-dimensional (3D) model systems and how to approach big imaging datasets
- Understand the basics of multivariate analysis on image-inferred features
- Ask our expert speaker questions and benefit from their knowledge and guidance.
The CellVoyager CQ1 provides the highest quality confocal images and extended live-cell imaging in a space-saving benchtop design.
CellVoyager CV8000 is the most advanced high-content screening system. The improved built-in incubator lets you analyze extended live cell responses. With its expandability, 4 cameras, 5 lasers and an optional built-in pipettor, the system permits increasingly complex assay development and high-content screening.
Through the use of measurement, control, information technology and life science, radically improve productivity across the entire value chain, from basic research to logistics and services.
We have 30 years of experience in this life science field and will respond to customer's problem solving with cutting-edge solutions.
Yokogawa’s high content analysis systems and dual spinning disk confocal technologies provide high-speed and high-resolution live cell imaging, enabling leading-edge research around the world.