Super-resolution optical microscopies have fundamentally changed the imaging landscape, providing life scientists with a suite of new research tools. However, such techniques can be difficult to implement, necessitating different compromises that can ultimately limit their applicability. Optical pixel reassignment provides a method for super-resolution that is implemented in the context of a traditional spinning disk confocal microscope.
The webcast Super-Resolution Spinning Disk Confocal Microscopy for Life Science Research provides an explanation of optical pixel reassignment followed by examples of how it is applied in cell and developmental biology research and discusses the use of “smart” automated standard-to-super-resolution imaging pipelines to unravel the mysteries of centrosome biogenesis and function, and how the molecular mechanisms underlying their function can go awry in disease states. In addition, it describes live imaging of neural stem cells using both traditional and super-resolution imaging to reveal the plasma membrane’s role in neurogenesis.
Attendees can expect to learn:
- Can super-resolution confocal microscopy capture dynamic processes in live cells?
- The difference between conventional and super-resolution spinning disk confocal microscopy systems.
- Types of research questions that can be addressed using super-resolution spinning disk confocal microscopy.
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