Single Cellome Unit SU10
Minimally Invasive Intracellular Nano-Injector
This system component automates the penetration and injection of single cells using a nanopipette.
It‘s low invasiveness enables manipulation of live single cells.
The system integrates with multiple manufacturers’ inverted microscopes.*1
Minimally Invasive Single Cell Analysis
- Low Invasiveness Adopted glass pipette with tip size of under 100 nm
- Automated Penetration Automated cell surface detection and penetration (Z direction movement)
- High Success Rate Approx. 95% success rate of injection*2
- Singe-Cell Targeting Enabled injection of selected cells under microscope observation
- Rapid Injection Capable of injecting one cell every 10 seconds
*1 Microscope sold separately.
*2 Experiment by Yokogawa.
Function to aspirate intracellular substances is under development.
Accurate Positioning
Long stroke movements and accurate positioning enabled by combining stepping motors and piezo electric actuator.
Accurate Positioning
Automatic cell detection and penetration with ion current measurement.
Injection Method
Utilizes electro-osmotic flow in the tip of the nanopipette to create a pump effect. The amount of injection is controlled by the duration of the voltage pulse application.
Automated Injection Process
Automatically perform approach, surface detection, injection and retraction of the tip of the nanopipette.
Application Example
- Direct injection of substances such as vector and genome editing tools (CRISPR/Cas9) into the nucleus
- Efficacy/toxicity evaluation of drug candidate molecules
- Other physical injection of reagents and proteins
Fast Injection with High Success Rate
By automating the steps to penetrate the target cell, an injection speed of approximately 10 seconds has been achieved.
Fluorescence was observed in 208 out of 220 (94.6%) HeLa cells where the fluorescent protein was injected (experiment by Yokogawa)
Below: RFP was injected into the HeLa cells, and sequentially observed with fluorescence.
Low-Invasive Injection
The extremely small tip diameter of the nanopipette minimizes damage to the target cell.
Below: RFP was injected into the HeLa cells, and sequentially observed with fluorescence.
Specification
| Item | Specification | |
|---|---|---|
| Basic function | Injection | By electroosmotic flow at tip of nanopipette |
| 3-axis manipulator | Coarse movement（Stepping Motor） | Stroke:50mm/axis（setting resolution: 0.625μm） |
| Fine movement (Piezoelectric Element） | Stroke:100μm/axis（setting resolution: 10nm） | |
| Electronics head | Voltage generation range | -10V～+10V（setting resolution：10mV） |
| Current measurement range | -100nA～+100nA | |
| External dimensions and weight | Main Controller | 260(W) x 99(H) x 280(D) mm, approx. 2.5Kg |
| Piezoelectric Element Controller | 236(W) x 88(H) x 273(D) mm, approx. 4.6kg | |
| 3-axis Manipulator | 280(W) x 220(H) x 190(D) mm, approx. 2.5kg | |
| Electronics Head | 35(W) x 27(H) x 95(D) mm, approx. 0.2kg | |
| Joystick | 100(W) x 135(H) x 135(D) mm, approx. 0.5kg | |
| Power consumption | Main Controller ＋ Piezoelectric Controller | Max 100W |
| Ambient conditions for operation | 15～35℃、20～70%RH without condensation | |
Brochures
- Single Celome Unit SU10 (3.6 MB)