Analysis Tutorial for CellPathfinder - Calcium Imaging-

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Calcium Imaging

In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

This method is effective for data in which cells show little movement and some variation in brightness, as seen in the images below. In this technique, the same cell regions are utilized across time points to perform graphing using fluorescence intensity.

Calcium Imaging

CellPathfinder software

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CellPathfinder is designed for our HCA systems, CQ1 and the CellVoyager series. From beginners to experts, the analysis software lets you quantify subtle physiological changes and even label-free samples with various graph options.

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