From Atom to Animal: Decoding the Multi-Scale Mechanisms of Influenza Entry

Webinar Introduction

This webinar explores a multi-scale approach to Influenza A Virus (IAV) entry, following our research philosophy that spans "from atom to animal. " We examine how IAV subverts host machineries—identified through unbiased RNAi and chemoproteomic screens—to regulate the sequential stages of uptake, uncoating, and genome delivery. Using automated spinning-disk confocal (CQ1) and super-resolution (CSU-W1 SoRa) microscopy, we quantify these virus cell entry dynamics in vitro before validating our findings with in vivo mouse models.
By pairing high-speed fluorescence with advanced image analysis, we quantify individual virion diffusion and real-time membrane remodeling during viral endocytosis. We will also discuss the future aim of achieving unprecedented spatiotemporal resolution by further developing the Virus-View Dual Confocal and Atomic Force Microscopy (ViViD-AFM) platform to integrate the Yokogawa CSU-W1 SoRa. Decoding these intricate virus-host interfaces is pivotal for the rational design of next-generation broad-spectrum antivirals.

Key topics include:

• Multi-scale analysis elucidates how Influenza A virus exploits host machinery during cell entry.
• RNAi and chemoproteomic screens identify host factors governing uptake, uncoating, and genome delivery. 
• High-speed CQ1 and CSU-W1 SoRa microscopy enable quantitative analysis of virion dynamics and membrane remodeling during endocytosis.
• ViViD-AFM development aims to extend spatiotemporal resolution for antiviral research.