Radiation-Induced Lipid Remodeling at the Single-Cell Level Using LC-MS

Overview

The importance of multi-omics research at single-cell and sub-cellular levels has grown significantly in recent years, especially for developing cancer treatments. Single Cellome System SS2000 supports this research by enabling sampling of single cells or cellular compartments in a controlled environment with simultaneous confocal imaging. For downstream applications, cells can be sampled directly into analytical vessels for storage, shipping, or immediate analysis. We introduce a method where cells exposed to high X-ray doses were incubated for several days while lipid droplet size changes were tracked by confocal imaging. Later, cells were sampled, and lipid composition and abundance were analyzed by LC-MS/MS and LS-MS, respectively.

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Single Cellome Application Note

 

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