Life Science

Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.

Yokogawa held our annual “Yokogawa CSU Spinning Disk Image Competition.” To highlight the research and advancements of students and scientists who participated in MBL,

First annual Yokogawa CSU Spinning Disk Image Competition at MBL 2023

The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.

Comparison between CSU and conventional LSM in 4D movies.

To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.

The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

High-content screening is a crucial technique in drug development, utilized both in the initial phases for identifying new candidate compounds and in later phases for assessing the pharmacological effects and safety of these candidates. This report highlights three applications using Yokogawa’s CellVoyager CV8000 and CQ1 high-content screening systems, paired with Yokogawa’s CellPathfinder analysis software, creating an integrated and user-friendly platform for drug discovery.

Featured Assays

1. Hepatotoxicity Assay: Evaluating the effect of vitamin K on HepG2 cells.
2. Cell Migration Assay: Investigating the impact of mitomycin C on cell migration.
3. In Vivo Tumor Metastasis: Studying tumor metastasis using zebrafish models.

This "Tutorial" provides overview of this software, from installation through data analysis.

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.

In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.

In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.

In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.

In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.

In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.

In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.

The water and wastewater works around the world have to respond to a wide range of challenges.
Here we introduce Yokogawa‘s three initiatives to address these issues and challenges.

Explore single-cell lipidomics and cell diversity with Yokogawa’s SS2000 in this on-demand webinar. Featuring Kyle Saunders from the University of Surrey’s SEISMIC facility, learn mass spectrometry insights and more—watch now!

Discover how generative AI and automation are revolutionizing neuroscience drug discovery by enabling scalable, high-throughput human iPSC neuronal models with unprecedented efficiency and translatability, reducing the reliance on less relevant cell lines.

In this webinar, you will learn:

• How to scale and standardize iPSC-derived neuronal models to overcome challenges in complexity, reproducibility, and throughput;
• How integrating iPSC neuronal models early in drug discovery can reduce reliance on less relevant cell lines and improve research translatability;
• How the Neuron Factory platform uses automation, brightfield microscopy, and generative AI to accelerate high-throughput screening and medicinal chemistry workflows.

Physiologically relevant 3D cell models are essential for drug discovery and preclinical research due to their functional and architectural similarity to solid tumors. One of the challenges faced by researchers is that many of the assays using these precious samples tend to be manual and tedious.

Using proprietary microfluidics technology, Protein Fluidics has created the Pu·MA System for automated complex 3D cell-based assays. In this webinar, application scientist Dr. Katya Nikolov will present her work on combining this novel automation technology with Yokogawa’s high-content imaging systems for biomarker detection in 3D cell models. Nikolov will demonstrate the utility of an automated immunofluorescence staining workflow followed by confocal imaging within the Pu·MA System flowchips. This automated workflow enables quantitative assessment of biomarkers which provides valuable data for further understanding disease mechanisms, preclinical drug efficacy studies, and in personalized medicine.

This webinar will explore:

• The Pu·MA System and novel technology for automated 3D cell-based assays
• How to perform automated immunofluorescence staining for biomarkers with a “hands-off” assay workflow
• How to visualize biomarkers after the assay with high-content imaging within the flowchip