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Life Science

  • Confocal Scanner Unit, CSU series, have been improved from the original CSU10 to the most recent CSU-X1, which are widely recognized as the de facto standard tool for live cell imaging, due to fast scanning and low photo-bleaching capability. CSU-W1 is our answer to the researchers’ request for “Wider FOV” and “Clearer Images”.

  • Cell Voyager 7000S (CV7000S) is a high content screening system with our original confocal scanner unit, a live cell stage incubator and a build-in liquid handler which enable long term live cell imaging and rapid kinetic analysis.

  • Confocal Quantitative Image Cytometer CQ1 offers a new approach to image quantification.

  • Label-free Morphological Analysis Software

September 05,2017 Sales release : CellVoyagerTM CV8000 High-throughput Cytological Discovery System
January 19,2017

SLAS High-Content Screening Conference 2017

Tuesday, September 19, 2017 to Wednesday, September 20, 2017

Courtyard by Marriott Madrid Princesa, Calle de la Princesa 40 Madrid 28008 Spain (Booth no. 4)

We will exhibit our high-content analysis (HCA) systems at SLAS High-Content Screening Conference 2017. To meet the increasing demands for HCA, we have been providing LIVE CELL 3D HCA systems by our original technology. We will introduce our long-awaited NEW product, CellVoyager CV8000.

Find out more about SLAS High-Content Screening Conference 2017

January 19,2017

SLAS2017

Saturday, February 4, 2017 to Wednesday, February 8, 2017 (Exhibition is from Monday, February 6)

Walter E. Washington Convention Center 801 Mt. Vernon Place NW, Washington, DC USA (Booth No. 1504)

We will exhibit our High Content Analysis (HCA) systems at SLAS2017.
To meet the increasing demands for HCA, we have been providing LIVE CELL 3D HCA systems by our original technology. We will introduce our HCA systems, CellVoyager CV7000S and CQ1, and their applications.

Find out more about SLAS2017

April 04,2016

Poster presentation in 3D Cell Culture 2016, 19-21 April 2016, Konzerthaus Freiburg/Germany

Yokogawa Electric Corporation will present data obtained by our confocal image cytometer CQ1 in “3D Cell Culture 2016: How close to ‘in vivo’ can we get? Models, Application & Translation”. The poster will show the results of 3D live cell imaging and analysis of the migration and the network formation of HUVEC cells in a multilayered cell sheet. The results demonstrate that CQ1 is an excellent research tool in the field such as regenerative medicine and drug discovery screening.

*Data were provided from BioProcess Systems Engineering Lab., Dept.Biotech., Grad. Sch. Eng., Osaka University.

Poster presentation in 3D Cell Culture 2016

February 10,2016 Yokogawa Concludes Distribution Agreement with Optec, LLC for Sale of Confocal Quantitative Image Cytometer CQ1 at the markets of OPTEC activity
October 01,2015 Sales release : Label-free Morphological Analysis Software CellActivision

 

Spinning disk confocal

microlens / fastsacnning / minimal photo bleach / high resolution

Microlens-enhanced Nipkow Disk Technology

Microlens-enhanced Nipkow Disk Technology

Fast scanning

point scanning

Point Scanning
1 line scan time=1[ms]
1000 lines/image
Scan lines=1000 [lines]
1×1000=1000 [ms]

disk scanning

Disk Scanning by CSU
Rotation Speed=10000 [rpm]=41.7[rps]
30°Rotation/image
1÷( 41.7×30/360 )= 0.5 [ms]

rotation

CSU raster scans a field of view when rotated 30 degrees:
360/30= 12scans/rotation

Scanning Speed and Resolution
Scanning Speed (fps) Resolution
CSU-W1 CSU-X1

Conventional
Line Scanning

Conventional
Point Scanning
1 2000
More than 2000×2000
1000
More than 1000×1000
512
512×512
512
512×512
30 2000
More than 2000×2000
1000
More than 1000×1000
500
512×512
64
64×64
100 2000
More than 2000×2000
1000
More than 1000×1000*1
512
512×512
impossible
500 impossible 1000
More than 1000×1000*1
100
512×100
impossible
1000 impossible 1000
More than 1000×1000*1
512
512×50
impossible
2000 impossible 1000
More than 1000×1000*1
512
512×50
impossible

※1 :In use of CSU-X1(Highend model) 

Minimal photobleaching/laser damages

Comparison between CSU and conventional LSM in 4D movies
MDCK cells stably expressing GFP-Rab25 were imaged at 2/s for 100s.
XYT-volume movies show apparent difference in the endosome fluorescence decay between the two systems.

CSU:Minimal bleaching over 100s


Conventional LSM:
Photo bleaching of each endosome is apparent

 

The CSU system is capable of reproducing the highest SNR (signal to noise ratio) measured for an SPSC system(single-beam confocal) at approximately 1/15th of the rate of the photobleaching.
 
It has been widely recognized among CSU system users, most typically cell biology investigators that extended imaging studies using the CSU10 system are remarkably free of photobleaching. Suggestions were made that its superior performance results from the high efficiency of its CCD detector or from the low illumination dosage of a spinning disc system
 
K.W.Dunn et al.,(Department of Medicine, Division of Nephrology, Indiana University Medical Center) thoroughly investigated photon economy of CSU system in comparison with that of conventional point scanning confocal systems.
 
(Journal of Microscopy, Vol. 218, Pt 2 May 2005, pp. 148 -159ng: Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems”)
 
High-speed Imaging of endosomes in living MDCK cells.

Cells expressing GFP-Rab25, a vesicle-associated protein, imaged at 20 f/s for 30s. 3X actual speed.)


Cells incubated in TexasRed-labelled transferrin which is internalized into endosomes.
Imaged at 11 f/s for 100s, 3X actual speed. Each endosome can be imaged with high S/N.


XYT volume movie of endosomes: Vertical axis represents time.
Lack of photobleaching in each endosome over time is apparent.

They quantitatively compared optical efficiency between the CSU system against several single point scanning confocal (SPSC) microscope systems, by measuring the photon economy by the signal to noise ratio(SNR) of images returned for a given level of photobleaching.

Their conclusions are:
*At moderately high imaging rates, the CSU10 system is capable of reproducing the highest SNR measured for an SPSC system at approximately 1/15th of the rate of the photobleaching.

*At higher levels of illumination, CSU system is capable of collecting images with SNRs four (4)-fold higher than the highest observed with the SPSC systems.

*Significant fluorescence saturation was found in the SPSC system, but not in the CSU10 system.

Not only is the CSU10 capable of collecting images much more rapidly than SPSC systems, it does so with much higher efficiency. The performance advantage of the CSU10 system derives not only from its more efficient collection, but also from more efficient excitation made possible by an illumination system that maximizes the population of fluorophores in the ground state, effectively optimizing the amount of fluorescence stimulated from a given number of fluorophore molecules.

Together, these characteristics support extended 4D imaging of living cells at rates sufficient to capture the 3D motion of intracellular vesicles moving up to several micrometers per second. .( K.W.Dunn et al. 2005) : Copyright 2005 The Royal Microscopical Society

High resolution

Multi-pinhole scanning with the CSU gives excellent lateral and axial resolution at high speed!

resolution

Lateral resolution

Comparison of single-shot (raw) images between CSU/confocal image and epifluorescence image (HeLa Cell expressing YFP, taken with Hamamatsu Orca 1344X1024,33msec exposure:
Image taken by Dr. T.Nagai, Hokkaido Univ.(in Japanese)

 

csuimage

Single Exposure Image (CSU)

pointscanimage

Single Exposure Image (Epifluorescence)

Enter the new world of High Content Screening with the highest-resolution imaging!

CellVoyager™ is a high-throughput cytological discovery system capable of high-speed and high-resolution imaging and analysis of biological reactions in live cells; an unsurpassed tool for effective drug development, compound evaluation,cytological functional studies, and more.

High Content Analysis

HCA is an automated research method that uses image analysis to closely examine target cells in chronological order based on single or multiple parameters. Its wide potential for investigating complex life events and phenotypes makes HCA a most promising tool for next generation drug discovery and pharmaceutical research.

  HTS Flow cytometry Microscopy
Strength High-speed handling of a large number of samples Acquires numeric data on individual cells Detailed observation of cellular events
Weakness Calculates only averages for wells Adherent cells must be removed Slow

HCA system Combines the strengths of all these conventional methods!↓

HCA system

Strength

  • Very rapidly analyzes a large number of samples
  • Acquires numeric data on individual cells
  • Detailed observation of cellular events
  • dherent cells can be observed as is
  • Temporal analysis of live cells
  • Automated high-speed imaging and analysis

Comparison of CSU series

Model CSU-W1 CSU-X1 CSU22*1 CSU10*
High-end Basic
Imaging Speed
(Max. fps)
200 2,000 360 1,000 360
Scanner Motor
Rotation Speed
(rpm)
1,500-
4,000
1,500-
10,000
(Variable)
1,800
(Fixed)*2
1,500-
5,000
(Variable)
1,800
(Fixed)
Recommended
camera
exposure time
5msec 0.5msec 33msec 1msec 33msec
Effective FOV 17x16mm 10x7mm
Disk unit Selectable up to
2 disks
Pinhole size :
50µm, 25µm
1 disk
Pinhole size :
50µm
Rotation
position trigger
signal
External signal
output possible
None*2 External signal
output possible
None
Filters EX Option Supplied
up to
3 filters
Supplied
1 filter
DM Option
(up to 3 filters)
Option
(1 filter)
Supplied
up to
3 filters
Supplied
1 filter
EM Option
(up to 10 filters
with filter wheel)
Option
(up to 6 filters
with filter wheel)
Option
(1 filter)
Supplied
up to 3 filters
Supplied
1 filter
Addition or
exchange of
filters
At user site :
DM block
and filters (EX, EM.)
At Yokogawa factory :
DM
At Yokogawa
factory
At user site
*1 discontinued *2 option

Comparison between CSU and other confocal systems

Model CSU Conventional
Point-scan Confocal
Conventional
Slit-scan Confocal
Other spinning
disk Confocal
Epi- fluorescence
(Wide field)
Scan Type Microlens-enhanced
multi beam scan
Single beam scan Line Scan Disk scan (Multi beam or slit) None
Scanner Motor
Rotation Speed
(rpm)
1,500-4,000 1,500-10,000
(Variable)
1,800
(Fixed)*2
1,500-5,000
(Variable)
1,800
(Fixed)
Light Source Lasers Hg or Xenon arc lamp
Detector CCD, EMCCD PMT Line CCD CCD, EMCCD
Microscope Flexible Specific Flexible / Specific Flexible
Scan speed of
full-size image
2000fps*1 ~1fps ~120fps (512X512) <200fps Any
Photo bleaching/
photo toxicity
Low Severe Low
Confocality High (X-Y-Z confocal) Modest
(Compromise
y-resolution)
Modest
(X-Y-Z confocal with pinhole,
Compromise y-resolution
with slit-scan)
None
Image Quality
(Background)
High Good S/N
(Low background)
High
(multiple averaging
necessary)
Modest Modest
(High background
with dim samples)
Low
(High background)
*1 discontinued *2 option

Comparison of Cellvoyager series

We offer three products models in the CellVoyager series. CV7000 and CV6000 are fully integrated, ALL-IN-ONE system, consisting of imaging system and analysis unit, so as to enable highly efficient screening without difficulties in connecting various instruments. CV1000 is a desk-top, ALL-IN-ONE live cell confocal system suitable for both research and small-scale HCA.

Model CV7000 CV6000 CV1000
Sample format Wellplate
6,24,96,384,
1536well
Wellplate
6,24,96,384well
35mm dish
Slide glass
Cover glass
chamber
Read time of
a 96 wellplate
60sec 240sec
Imaging mode Confocal,
Epi-fluorescence,
Bright field*1,
Phase-contrast*1
Confocal,
Epi-fluorescence,
Bright field
Excitation wave length Selectable from
405,488,561,640
up to 4 lasers
Selectable from
405,488,
532(561),640
up to 4 lasers
Selectable from
405,488,561
up to 3 lasers
Bright field*1 Halogen lamp LED
Camera Type sCMOS
Max.3 Cameras
EMCCD
Max.3 Cameras
EMCCDC or
Cooled CCD
No. of pixcels 2560*2160 512*512 EMCCD
512*512
or1024*1024
Cooled CCD
1344*1024
Image
area(mm)
16.64*14.04 8.19*8.19 EMCCD:
8.19*8.19
Cooled CCD
8.67*6.60
Incubator*1 CO2 supply box
(CO2: 5%, Humidifier)
Temperature range:
35 up to 40ºC
Stage incubator
(CO2: Off,
1-9%, Humidifier)
Temperature range:
30 up to 40ºC
Analysis software

Analytical protocol:
Granularity, Neurite Outgrowth,
Nuclear Morphology,
Nuclear Translocation,
Plasma Membrane Translocation

-
Option Dispenser,Plate loaders/lifter,
Bar-code reader
Pinhole change
unit(25um,50um)

Useful Sites

1) Microscopy & Imaging Resources on the WWW
Complete list of all aspects of microscopy & imaging, by Douglas W. Cromey From Cellular Imaging Core of Southwest Environmental Health Sciences Center, University of Arizona College of Pharmacy, University of Arizona

2) The Centro de Biologia Molecular “Severo Ochoa” (CBMSO)
Contains links to general information, microscopy laboratories, publications, courses and meetings, societies, images. Especially useful for finding microscopy workshops.

3) The Cell Imaging Facility, a part of the University of Utah Health Science Center's Core Research Facilities Department
Good explanation of CSU-Disk scanning confocal system by Chris Rodesch

4) Molecular Expressions Website
Run by National High Magnetic Field Laboratory, Florida State University.
One of Web's largest collections of excellent optical microscopy images, and quite thorough information on all types of microscopy.
Interactive Java Tutorials sponsored by Nikon (Nikon MicroscopyU) and Olympus(Olympus Microscopy Resource Center) are extremely useful to learn not only confocal but all kinds of microcopies, and related technologies.

Life Science Textbooks

Microscopy Techniques, Advances in Biochemical Engineering/Biotechnology Vol.95
Serial Editor T. Scheper, Volume Editor J. Rietdorf, Springer(2005)
ISBN-10 3-540-23698-8
Confocal Microscopy for Biologists
Edited by Alan R.Hibbs,Kluwer Academic / Plenum Publishers(2004)
ISBN:0-306-48468-4(hardback) 0-306-48565-6(e-Book)
Live Cell Imaging, A Laboratory Manual
Edited by Robert D. Goldman & David L. Spector. 2nd Edition,Cold Spring Harbor Laboratory Press (2010)
ISBN:0-87969-893-4(pbk), ISBN 0-87969-892-6 (hardcover)
Handbook of Biological Confocal Microscopy, 3rd Edition
Edited by James B. Pawley, Springer(2006)
VideoMicroscopy, The Fundamentals
Shinya Inoue, Kenneth Spring, Second Edition Plenum Press. New York,(1997)
ISBN: 0-306-45531-5
Direct-View High-Speed Confocal Scanner: The CSU-10, Chapter 2: Cell Biological Applications of Confocal Microscopy (Methods in Cell Biology)
shinya Inoue and Ted Inoue
Edited by Brian Matsumoto Academic Press
ISBN:0-12-580445-8 ; 2nd Rev (2002/12)

Articles:  CSU Technology and its applications

Quantification and clustering of actin cytoskeletal structures in plant cells: role of actin bundling in stomatal movement during diurnal cycles in Arabidopsis guard cells.
Higaki T, Kutsuna N, Sano T, Kondo N, Hasezawa S
submitted.
Current Application and Technology of Functional Multineuron Calcium Imaging.
Shigehiro Namiki and Yuji Ikegaya
Biological and Pharmaceutical Bulletin Vol.32(2009) , No.11
Live imaging of yeast Golgi cisternal maturation.
Kumi Matsuura-Tokita, Masaki Takeuchi, Akira Ichihara, Kenta Mikuriya and Akihiko Nakano
Nature 441, 1007-1010 (22 June 2006)
Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems.
E. Wang, C. M. Babbey & K. W. Dunn
Journal of Microscopy, Vol. 218, Pt 2 May 2005, pp. 148 ?159
Optically sectioned fluorescence lifetime imaging using a Nipkow disk microscope and a tunable ultrafast continuum excitation source.
D.M.Grant, D.S. Elson, D.Schimpf, C.Dunsby, J.Requejo-Isidro, E.Auksorius, I.Munro, M.A. A. Neil, P. M. W. French ,E. Nye G. Stamp, P.Courtney
Optics Letters Vol. 30, No. 24 (2005 ) 3353
Optimization of Spinning Disk Confocal Microscopy: Synchronization with the Ultra-Sensitive EMCCD.
F.K.Chong, C.G.Coates, D.J.Denvir, N.McHale, K.Thornbury & M.Hollywood
Proceedings of SPIE 2004
Spinning disk confocal microscope system for rapid high-resolution, multimode, fluorescence speckle microscopy and green fluorescent protein imaging in living cells.
Maddox PS, Moree B, Canman JC, Salmon ED
Methods Enzymol. 360:597-617 (2003)
A high-speed multispectral spinning-disk confocal microscope system for fluorescent speckle microscopy of living cells.
Adams, M.C., Salmon, W.C., Gupton, S.L., Cohan, C.S., Wittmann, T., Prigozhina, N. & Waterman-Storer, C.M
Methods, 29, 29?41 (2003)
Spinning-disk confocal microscopy ? a cutting-edge tool for imaging of membrane traffic.
Nakano, A.
Cell Structure Function, 27, 349?355.(2002)
High Speed 1-frame/ms scanning confocal microscope with a miclolens and Nipkow disks.
T.Tanaami, S.Otsuki,N.Tomosada, Y.Kosugi. M.Shimizu & H.Ishida
Applied Optics, Vol.41,No.22(2002)
New imaging modes for lenslet-array tandem scanning microscopes.
T. F. Watson, R. Juskaitis & T. Wilson
Journal of Microscopy, Vol. 205, Pt 2 February (2002) 209?212
High-speed confocal fluorescence microscopy using a Nipkow scanner with microlenses for 3-D imaging of single fluorescence molecule in real time.
A.Ichihara, T.Tanaami, K.Isozaki, Y.Sugiyama, Y.Kosugi, K.Mikuriya, M.Abe and I.Uemura
Bioimages 4, 57-62(1996)

Articles:  Cell Biology

(Vesicular transport, actin dynamics, microtubule dynamics, cell division)

Long-term, Six-dimensional Live-cell Imaging for the Mouse Preimplantation Embryo That Does Not Affect Full-term Development.
Yamagata, K., Suetsugu, R. and Wakayama, T., J. Reprod. Dev., 55: 328-331(2009) ”
The Caenorhabditis elegans DDX-23, a homolog of yeast splicing factor PRP28, is required for the sperm-oocyte switch and differentiation of various cell types.
Konishi, T., Uodome, N., and Sugimoto, A.,Developmental Dynamics 237, 2367-2377(2008)
Determining the position of the cell division plane.
J. C. Canman, L. A. Cameron, P.S. Maddox, A. Straight, J, S. Tirnauer, T. J. Mitchison, G. Fang., T. M. Kapoor & E. D. Salmon, Nature 424, 1074-1078 (28 August 2003) “Cover”
Taxol-stabilized Microtubules Can Position the Cytokinetic Furrow in Mammalian Cells.
Katie B. Shannon, Julie C. Canman,C. Ben Moree,Jennifer S. Tirnauer, and E. D. Salmon, Mol Biol Cell. September; 16(9): 4423?4436 (2005)
Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase.
G. C. Rogers, S. L. Rogers, T. A. Schwimmer,S. C. Ems-McClung, .C E. Walczak, R. D. Vale, J. M. Scholey & D. J. Sharp, Nature 427(6972):, 364-370 (2004)
Crm1 is a Mitotic Effector of Ran-GTP in Somatic Cells
Arnaoutov, A., Azuma, Y., Ribbeck, K., Joseph, J., Boyarchuk, Y. and Dasso, M, Nat Cell Biol. Jun;7(6):626-32 (2005).
Nuclear congression is driven by cytoplasmic microtubule plus end interactions in S. cerevisiae.
J.N. Molk, E.D. Salmon, and K. Bloom, JCB, Vol. 172, Number 1, 27-39 (2006)
Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
Nasser M. Rusan and Patricia Wadsworth ,JCB 168 (1) 21?28 (2005)
The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line.
G. Goshima and R.D. Vale,JCB 162(6) 1003?1016 (2003)
Spindle orientation in Saccharomyces cerevisiae depends on the transport of microtubule ends along polarized actin cables.
Hwang, E., Kusch, J., Barral, Y. & Huffaker, T.C, J. Cell Biol. 161, 483?488 (2003)
Cell migration without a lamellipodium : translation of actin dynamics into cell movement mediated by tropomyosin
S.L. Gupton, K.L. Anderson, T. P. Kole, R.S. Fischer, A. Ponti, S.E. Hitchcock-DeGregori, G. Danuser, V.M. Fowler, D.Wirtz, D. Hanein, and C.M. Waterman-Storer ,JCB 168(4) 619-631(2005)
Actin dynamics in the contractile ring during cytokinesis in fission yeast.
Pelham, R.J. & Chang, F, Nature, 419, 82?86. (2002)
CD8+ effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity.
Nishimura S, Manabe I, Nagasaki M, Eto K, Yamashita H, Ohsugi M, Otsu M, Hara K, Sugiura S,Yoshimura K, Kadowaki T, Nagai R, Nature Medicine 8, 914- 920 (15 August 2009)
T-cell engagement of dendritic cells rapidly rearranges MHC class II transport.
M. Boes, J. Cerny, R. Masso, M. Op den Brouw, T. Kirchhausen, J. Chenk & H. L. Ploegh, Nature 418, 983- 988 (29 August 2002) “Cover”
Functional coordination of intraflagellar transport motors.
G. Ou, O.E. Blacque, J.J.Snow, M.R. Leroux & J.M. Scholey,Nature 436(7050):583-7. (2005).
Three-dimensional analysis of post-Golgi carrier exocytosis in epithelial cells
Kreitzer, G., Schmoranzer, J., Low, S.H., Li, X., Gan, Y., Weimbs, T., Simon, S.M. & Rodriguez-Boulan, E, Nature Cell Biol. 5, 126?136. (2003)
Dynamics of Membrane Clathrin-Coated Structures During Cytokinesis.
James H. & Wang, Yu-Li Warner, Anne K., Keen, Traffic 7 (2), 205-215. (2006 ) 

Articles:  Neuroscience

Activity-induced targeting of profilin and stabilization of dendritic spine morphology.
Ackermann, M., and A. Matus, Nat Neurosci. Nov;6(11):1194-200 (2003)
Dynamics and Regulation of Clathrin Coats at Specialized Endocytic Zones of Dendrites and Spines.
T.A. Blanpied, D.B. Scott, M.D. Ehlers, Neuron, Vol. 36, 435?449, October 24(2002)
Neurabin/Protein Phosphatase-1 Complex Regulates Dendritic Spine Morphogenesis and Maturation.
R.T.Terry-Lorenzo, D.W. Roadcap, T. Otsuka , T.A. Blanpied, P.L. Zamorano, C.C. Garner, S. Shenolikar, and M.D. Ehlers, MBC Vol. 16, Issue 5, 2349-2362, May (2005)
Phosphatidylinositol phosphate kinase type I regulates dynamics of large dense-core vesicle fusion.
L.W..Gong , G. D.Paolo, E. Diaz ., G.Cestra , M-E. Diaz, M. Lindau , P.De Camilli , and D. Toomre , PNAS, vol. 102 no. 14 5204-5209 (2005)

Calcium Dynamics

Formation of planar and spiral Ca2+ waves in isolated cardiac myocytes.
Ishida, H., Genka, C., Hirota, Y., Nakazawa, H. & Barry, W.H, Biophys. J. 77, 2114?2122 (1999)
Simultaneous imaging of phosphatidyl inositol metabolism and Ca2+ levels in PC12h cells.
Morita,M,Yoshiki, F,and ,Kudo,Y, BBRC 308, 673-678 (2003)
Calcium oscillations in interstitial cells of the rabbit urethra.
Johnston, L., Sergeant, G. P., Hollywood, M. A., Thornbury, K. D. & McHale, N. G,The Journal of Physiology 565 (2), 449-461 (2005).

Microvascular Blood Flow

Real-time observation of hemodynamic changes in glomerular aneurysms induced by anti-Thy-1 antibody.
Oyanagi-Tanaka, Y., Yao, J., Wada, Y., Morioka, T., Suzuki, Y., Gejyo, F., Arakawa, M. & Oite, T, Kidney Int. 59, 252?259. (2001)
Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse.
Shahrokh Falati, Prter Gross, Glenn Merrill-Skoloff, Barbara C. Furie & Bruce Furie, Nat Med 8 (10) 1175-1180(2002)

Other Applications

Evidence of ROS generation by mitochondria in cells with impaired electron transport chain and mitochondrial DNA damage
Hiroko P. Indo,Mercy Davidson,Hsiu-Chuan Yen,Shigeaki Suenaga,Kazuo Tomita,Takeshi Nishii,Masahiro Higuchi,Yasutoshi Koga,Toshihiko Ozawa,Hideyuki J. Majima,Mitochondrion No.7 106?118(2007)
Overview:

Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression

Overview:

Discovering the Basic Principles of Life through the Live Imaging of C. elegans

Overview:

Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.

Overview:

Use of the spinning disk confocal at the Harvard Medical School microscopy core.

Overview:

New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!

Overview:

Faster, Deeper, and Clearer -in vivo molecular imaging technology-

Overview:

On-site manipulation of protein activities: Understanding intricate cell signaling pathways.

Overview:

Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.

Overview:

Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.

Application Note
Overview:

CV1000 clears the hurdle in Live Cell Imaging
All-in-one Live cell imaging solution

Application Note
Overview:

Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System

Application Note
Overview:

Cell clusters are directly measured with high-throughput 3D imaging
Confocal Quantitative Image Cytometer

Overview:
  • Colony Formation
  • Scratch Wound
  • Cytotoxicity
  • Neurite Outgrowth
  • Co-culture Analysis
  • Cell Tracking
Application Note
Overview:

Wide and Clear
Confocal Scanner Unit

Overview:

Faster, Brighter, and More Versatile
Confocal Scanner Unit

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