Application: CSU-W1
- ES cell
- Zebrafish
- Brain slice
- Early-stage embryo
ES cell

Ocular cup organ regenerated from mouse ES cells
Data: Mototsugu Eiraku, Ph.D., and Yuiko Hasegawa, Ph.D., Sasai Lab., Organogenesis Neurogenesis group, Center for Developmental Biology, RIKEN

3D Play
MIP
YZ plane
| Measurement condition | |
|---|---|
| Z-section/stack | 100um(2um/51slices) |
| Fluorescent probe | 640nm:Cy5 |
| Pinhole | 25um |
| Objective lens | 20x dry |
ES cell colony
Data: Nozomu Takata, Ph.D., Sasai Lab., Organogenesis Neurogenesis group, Center for Developmental Biology, RIKEN

3D Play
MIP
| Measurement condition | |
|---|---|
| Z-section/stack | 50um(1um/51slices) |
| Fluorescent probe | GFP(Excitation:488nm) mCherry(Excitation:561nm |
| Pinhole | 50um |
| Objective lens | 60x oil |
Data: Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University

Timelapse(MIP)
Full-size movie Play
| Measurement condition | |
|---|---|
| Z-sections/ stack |
80um (0.4um/201slices) |
| Fluorescent probe | 488nm: H2B-EGFP 561nm: mCherry-MBD-NLS |
| Pinhole | 50um |
| Objective lens | 60x silicone |
| Total time | 490 mins (Interval:10mins) |
Zebrafish

Zebra fish embryo
Data: Makoto Suzuki, Ph.D., and Naoto Ueno, Ph.D., Division of Morphogenesis, National Institute of Basic Biology

3D reconstructed image of the whole embryo

Excerpts from Timelapse(MIP)
All timelapse Play

3D reconstructed embryo
(partial, at high magnification)

YZ Plane
| Measurement condition | |
|---|---|
| Z-Sections/ stack |
Upper left:99um(1um/100slices), Lower left:50um(0.5um/101slices), Right:1slice |
| Fluorescent probe | Left: membrane RFP(Excitation:561nm) Right: EB3-GFP(Excitation:488nm) membrane RFP(Excitation:561nm) |
| Pinhole | 50um |
| Objective lens | Upper left:20x dry,Lower left,right: 60x water |
Zebrafish
Real-Time Visualization of Neuronal Activity
Data: Akira Muto and Koichi Kawakami, Division of Molecular and Developmental Biology, National Institute of Genetics

Excerpts from Timelapse(MIP)
All timelapse movie: Play

Time-line MIP of timelapse images

3D

Larval zebrafish: The fifth day after fertilization
Pink area: Optic tectum
| Measurement condition | |
|---|---|
| Total time | 152 sec. (Stream: 600 images) |
| Excitation | 488nm: GCaMP |
| Pinhole | 50um |
| Objective lens | 40x water |
Reference: Muto et al., Real-Time Visualization of Neuronal Activity during Perception, Current
Biology 23(4):307-311
Brain slice

Brain slice of mouse fetus
Data: Atsunori Shitamukai, Ph.D., Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN

3D
| Measurement condition | |
|---|---|
| Z-sections/stack | 15um(0.5um/31slices) |
| Fluorescent probe | GFP(Excitation:488nm) RFP(Excitation:561nm) |
| Pinhole | 50um |
| Objective lens | 60x water LWD |
| Total time | 2hours (Interval:1min) |
Early-stage embryo

Early-stage mouse embryo
Data: Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University

Timelapse (MIP) | Full-size movie Play
| Measurement condition | |
|---|---|
| Z-sections/ stack |
100um (1um/101slices) |
| Fluorescent probe | 488nm: H2B-EGFP 561nm: mCherry-MBD-NLS |
| Pinhole | 50um |
| Objective lens | 60x silicone |
| Total time | 48 hours (Interval:10mins) |

Excerpts from Timelapse (MIP of chromosome)
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