New Era in Mammalian Genetics Research; Utilize the Same Embryo After Observation

다운로드 (3.0 MB)

In the fertilization and early embryonic development process, various events are spatiotemporally controlled, and many events are connected in the cause-effect relations toward the final goal of ontogenesis. To understand the mechanism of this process, conventional experimental techniques by fixing and destruction of the cells have limitations. If this process can be observed over time and the development process can be continued after the observation, it will open a new era in the Genetics research. A mammalian developmental biology researcher, Dr. Kazuo Yamagata, established such technique by using the CSU system.
He successfully imaged mouse embryos over a long period of time, from the post-fertilization through to the blastocyst stage, to acquire approximately 60,000 of 3D confocal images. Thereafter, the embryos were transferred to a recipient mouse, and the pups were born all normally, grew healthy, and were capable of reproduction; a firm evidence that this early embryo imaging technique does not adversely affect the process of full-term development. The high speed image acquisition and extremely low excitation light unique for the CSU system enabled greatly reduced phototoxicity and realized intensive but damage-free long time observation. Only by using this technology which does no harm on the embryonic development, it is possible to “utilize the same embryo after intensive analysis by imaging” , and thus to investigate cause- and-effect relationship of various early stage phenomena and their influence on the development.

Experimental flow
Movie example

Figure : The long-time, multi-dimensional live cell imaging on early stage embryos does not affect the process of ontogenesis.
(a) Experimental flow
(b) Movie example: Images were acquired at 7.5-minute intervals over approximately 70 hours.
      This figure shows extracted images at 2-hour intervals.
    Each image is the maximum intensity projection of a total of 51 images in the Z-axis direction.
    Green:Spindle (EGFP-α‒tubulin), Red:Nucleus (H2B-mRFP1)
 

Experimental conditions
Total time 70 hours
Interval 7.5 min/stack
Z-axis slices 51 sections (at 2μm intervals)
Channel 3(DIC, EGFP, mRFP1)
Position 6(Total 72 embryos)
Laser power(Measured at objective lens) 488nm (0.281 mW), 561nm (0.225 mW)


Data: Kazuo Yamagata, PhD., Laboratory for Genomic Reprogramming,Center for Developmental Biology, Riken


Our Social Medias

We post our information to the following SNSs. Please follow us.

  Follow us Share our application
•Twitter @Yokogawa_LS Share on Twitter
•Facebook Yokogawa Life Science Share on Facebook
•LinkedIn Yokogawa Life Science Share on LinkedIn

 

Yokogawa's Official Social Media Account List

Social Media Account List


Related Products & Solutions

  • High Speed

    The CSU-X1 is widely recognized as the leading tool for live cell imaging with 2,000 fps capability.

    See More
  • Life Science

    Yokogawa의 고밀도 분석시스템과 이중 회전 디스크 공초점 기술은 고속 및 고해상도 라이브 셀 이미징을 제공하여 전세계 최첨단 연구를 가능하게 합니다.

    See More
  • Spinning Disk Confocal

    듀얼 스피닝 디스크 기술의 선구자인 Yokogawa의 공초점 스캐너 장치는 광학 현미경을 변형시켜 실시간 라이브 셀 이미징 기술을 구현합니다. 

    See More

Top