CellVoyager CV8000 High-Content Screening System

CV8000의 셀 인큐베이터는 향상된 밀폐 설계로 장기간 셀의 움직임을 관찰할 수 있습니다. 또한 CV8000에는 레이블이 없는 셀의 이미지 및 샘플의 3D 이미지를 분석할 수 있는 새로운 프로그램인 CellPathfinder가 포함되어 있습니다. 이러한 기능을 통해 CV8000은 iPS 및 ES 세포와 같은 최신 주제에 대한 신약 개발 및 생체 의학 연구의 효율성을 향상시킵니다.

CV8000 System Highlights

Excitation laser wavelength 405 nm, 445 nm, 488 nm, 561 nm, 640 nm
Illumination source Laser
Objective lens 2x to 60x
(Dry, Phase contrast, Water immersion, Long working distance)
Camera High-sensitivity sCMOS camera (up to 4 units)
Autofocus Laser-based mode, image-based mode
Software CellPathfinder, CellLibrarian

레이블이 없는 셀 관찰 

CV8000은 DPC(digital phase contrast) 방법을 사용하여 레이블이 없는 셀을 관찰할 수 있어 고대비 이미지와 자세한 셀 정보를 제공합니다.

더 짧은 시간에 더 많은 이미지 캡쳐

CV8000에는 4개의 카메라가 있어서 동시에 캡처한 이미지 수를 늘리고 이미지 수집 시간을 단축하며 여러 색상의 이미지를 얻을 수 있습니다.

선명한 고화질 이미지 획득

Yokogawa의 독창적인 공초점 스캔 디스크 기술인 Nipkow 디스크를 기반으로 하는 CV8000은 형광 강도가 약한 셀의 관찰을 용이하게 합니다.

 

 

 

Over twenty years of experience

  • Yokogawa CSU confocal scanner unit has been selected by top scientists, and more than 2800 units*1 have been sold in the world.
  • High-speed and low-phototoxic imaging are achieved by microlens-enhanced Nipkow disk system.
     

25/50 μm pinhole disk exchanger※2.

  • Users can choose the pinhole disk matching to the purpose of the experiments.
  • Z-resolution in thick samples is improved by using 25 μm pinhole disk

    .


*1  As of September 2017

*2  Option
 

Highly accurate stage incubator and dispenser for time lapse

  • Stage incubator enables live cell imaging over 3 days without supplying additional water※1
  • Disposable tip type liquid dispenser for kinetic assays※2
  • Hypoxia experiments and FRET

Stage incubator performance

Calcium response

 

Fast kinetic responses can be visualized by applying drugs during time lapse recordings.
Ionomycin was applied to A10 cells labeled with Fluo-4 during recording with 100ms intervals.

High throughput

  • Wide-view sCMOS camera, up to 4 cameras
  • Approx. 1 minute to acquire 4-color images of 96 wells※3

Water immersion objectives

  • 40x and 60x water immersion objectives provide brighter and higher resolution images.
  • Automated water supply


※1 Depends on conditions and samples.
※2 Optional
※3 1 field/well, 4-camea simultaneous recording

High content analysis system CellPathfinder   Click Here For More Info!

  • Preset analysis menus for a variety of applications
  • Flexible graph functions to display analysis results
  • Direct link between chart and object imageMachine learning

Machine learning


Software learns the features of the sampleobjects collected by users.

3D analysis


 

Label-free analysis
DPC※1 function is a powerful tool to analyze unstained bright field samples.

※1 Digital phase contrast

High-throughput Cytological Discovery System

Model CV8000
Sample format Multiple well plate (6, 12, 24, 48, 96, 384, 1536 wells), glass slide
Image mode Confocal mode
max. 4 color simultaneous recording,
Brightfield mode, Phase-contrast
(10x, 20x for 6, 12, 24 well plates)
digital phase contrast (10x 20x)
Output data format Image data: 16bit TIFF, PNG
Numerical data: CSV, Original format
Excitation wavelength 405/445/488/561/640nm, all solid laser, max. 5 lasers
【Option】365nmLED
White light illumination LED
Autofocus Laser-based mode, Image-based mode
Objective lens Max. 6 lenses are available : Automatically switchable
Dry: 2x, 4x, 10x, 20x, 40x
Water immersion: 40x, 60x
Long working distance: 20x
Phase-contrast: 10x, 20x
Confocal unit Microlens-enhanced wide-view dual Nipkow disk confocal scanner, 50 μm pinhole
【Option】 25/50 μm pinhole disk exchanger
Camera sCMOS (effective pixels: 2000X2000 pixel size: 6.5 μm), max. 4 cameras
Stage incubator Temperature: 35-40℃ CO2 supply box (CO2: 5%, forced humidification)
Dispenser 【Option】Disposable tip type
(For 96 tip , 384 tip type)
Bar-code reader 【Option】1 or 2 dimension
Workstation Dual-monitor workstation for system control
Dual-monitor workstation for data analysis
Analysis software
(CellPathfinder)
Granularity, Neurite, Nuclear morphology, Nuclear translocation, Plasma membrane translocation, Machine learning, Label-free analysis, 3D analysis, Texture analysis, etc.
Operating environment 15-30ºC, 20-70%RH (No condensation)
Power supply Measurement unit: Single-phase 200VAC, 2KVAmax
Workstation for system control : 100VAC, 1.3KVAmax, Workstation for data analysis: 100VAC, 690VAmax
External dimension Measurement unit: 1280W×895D×1450H mm
Weight Measurement unit: 510Kg

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CV8000 Partner

North America, Europe

WAKO Automation USA, Inc.

WAKO Automation USA, Inc.
Recommended vendor for High Content Screening integration and automation solutions.

 

Asia

Tekon Biotech

Tekon Biotech
P.R.China, Hong Kong, Taiwan, Macao


Shanghai Genesci Medical technology

Shanghai Genesci Medical technology Co.,Ltd.
People's Republic of China (excluding Hong Kong, Taiwan, Macao)


LBD Life Sciences China Limited

LBD Life Sciences China Limited.
People's Republic of China (excluding Hong Kong, Taiwan, Macao)

Overview:

The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

Application Note
Application Note
Overview:

List of Selected Publications : CV8000, CV7000, CV6000

Application Note
Application Note
Overview:

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

Overview:

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

Overview:

In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.

Overview:

In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

Overview:

In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.

Overview:

In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.

Overview:

In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.

Overview:

In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.

Overview:

In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.

Overview:

In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.

Yokogawa Technical Report
Overview:

We have been developing a prototype of a genomic drug test support system using our CSU confocal scanner. This system administers chemical compounds that serve as potential drug candidates into living cells, which are the most basic components of all living organisms, records the changes in the amount and localization of target molecules inside cells with the CSU confocal scanner and a highly sensitive CCD camera, and processes and quantifies the captured high-resolution image data.

Yokogawa Technical Report
1.2 MB
Yokogawa Technical Report
6.1 MB
Yokogawa Technical Report
The World as Seen from Cells
(rd-te-r06002-001)
2.2 MB

Product Overviews

    Product Overview
    Overview:

    The CV8000 features a cell incubator with an improved airtight design that facilitates the observation of cell behavior over long periods of time. In addition, the CV8000 comes with CellPathfinder, a new program that can analyze images of unlabeled cells and 3D images of samples. With these features, the CV8000 improves the efficiency of drug discovery research and biomedical research on leading-edge subjects such as iPS and ES cells.

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