CellVoyager CV8000 High-Content Screening System
CV8000의 셀 인큐베이터는 향상된 밀폐 설계로 장기간 셀의 움직임을 관찰할 수 있습니다. 또한 CV8000에는 레이블이 없는 셀의 이미지 및 샘플의 3D 이미지를 분석할 수 있는 새로운 프로그램인 CellPathfinder가 포함되어 있습니다. 이러한 기능을 통해 CV8000은 iPS 및 ES 세포와 같은 최신 주제에 대한 신약 개발 및 생체 의학 연구의 효율성을 향상시킵니다.
CV8000 System Highlights
| Excitation laser wavelength | 405 nm, 445 nm, 488 nm, 561 nm, 640 nm |
| Illumination source | Laser |
| Objective lens | 2x to 60x (Dry, Phase contrast, Water immersion, Long working distance) |
| Camera | High-sensitivity sCMOS camera (up to 4 units) |
| Autofocus | Laser-based mode, image-based mode |
| Software | CellPathfinder, CellLibrarian |
레이블이 없는 셀 관찰
CV8000은 DPC(digital phase contrast) 방법을 사용하여 레이블이 없는 셀을 관찰할 수 있어 고대비 이미지와 자세한 셀 정보를 제공합니다.
더 짧은 시간에 더 많은 이미지 캡쳐
CV8000에는 4개의 카메라가 있어서 동시에 캡처한 이미지 수를 늘리고 이미지 수집 시간을 단축하며 여러 색상의 이미지를 얻을 수 있습니다.
선명한 고화질 이미지 획득
Yokogawa의 독창적인 공초점 스캔 디스크 기술인 Nipkow 디스크를 기반으로 하는 CV8000은 형광 강도가 약한 셀의 관찰을 용이하게 합니다.
Over twenty years of experience
- Yokogawa CSU confocal scanner unit has been selected by top scientists, and more than 2800 units*1 have been sold in the world.
- High-speed and low-phototoxic imaging are achieved by microlens-enhanced Nipkow disk system.
25/50 μm pinhole disk exchanger※2.
- Users can choose the pinhole disk matching to the purpose of the experiments.
- Z-resolution in thick samples is improved by using 25 μm pinhole disk
.
*1 As of September 2017
*2 Option
Highly accurate stage incubator and dispenser for time lapse
- Stage incubator enables live cell imaging over 3 days without supplying additional water※1
- Disposable tip type liquid dispenser for kinetic assays※2
- Hypoxia experiments and FRET
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Stage incubator performance |
Calcium response |
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Fast kinetic responses can be visualized by applying drugs during time lapse recordings. |
High throughput
- Wide-view sCMOS camera, up to 4 cameras
- Approx. 1 minute to acquire 4-color images of 96 wells※3
Water immersion objectives
- 40x and 60x water immersion objectives provide brighter and higher resolution images.
- Automated water supply
※1 Depends on conditions and samples.
※2 Optional
※3 1 field/well, 4-camea simultaneous recording
High content analysis system CellPathfinder Click Here For More Info!
- Preset analysis menus for a variety of applications
- Flexible graph functions to display analysis results
- Direct link between chart and object imageMachine learning
Machine learning
Software learns the features of the sampleobjects collected by users.
3D analysis
Label-free analysis
DPC※1 function is a powerful tool to analyze unstained bright field samples.
※1 Digital phase contrast
High-throughput Cytological Discovery System
| Model | CV8000 |
|---|---|
| Sample format | Multiple well plate (6, 12, 24, 48, 96, 384, 1536 wells), glass slide |
| Image mode | Confocal mode max. 4 color simultaneous recording, Brightfield mode, Phase-contrast (10x, 20x for 6, 12, 24 well plates) digital phase contrast (10x 20x) |
| Output data format | Image data: 16bit TIFF, PNG Numerical data: CSV, Original format |
| Excitation wavelength | 405/445/488/561/640nm, all solid laser, max. 5 lasers 【Option】365nmLED |
| White light illumination | LED |
| Autofocus | Laser-based mode, Image-based mode |
| Objective lens | Max. 6 lenses are available : Automatically switchable Dry： 2x, 4x, 10x, 20x, 40x Water immersion： 40x, 60x Long working distance： 20x Phase-contrast： 10x, 20x |
| Confocal unit | Microlens-enhanced wide-view dual Nipkow disk confocal scanner, 50 μm pinhole 【Option】 25/50 μm pinhole disk exchanger |
| Camera | sCMOS (effective pixels: 2000X2000 pixel size: 6.5 μm), max. 4 cameras |
| Stage incubator | Temperature: 35-40℃ CO2 supply box (CO2: 5%, forced humidification) |
| Dispenser | 【Option】Disposable tip type (For 96 tip , 384 tip type) |
| Bar-code reader | 【Option】1 or 2 dimension |
| Workstation | Dual-monitor workstation for system control Dual-monitor workstation for data analysis |
| Analysis software (CellPathfinder) |
Granularity, Neurite, Nuclear morphology, Nuclear translocation, Plasma membrane translocation, Machine learning, Label-free analysis, 3D analysis, Texture analysis, etc. |
| Operating environment | 15-30ºC, 20-70%RH (No condensation） |
| Power supply | Measurement unit: Single-phase 200VAC, 2KVAmax Workstation for system control : 100VAC, 1.3KVAmax, Workstation for data analysis: 100VAC, 690VAmax |
| External dimension | Measurement unit: 1280W×895D×1450H mm |
| Weight | Measurement unit: 510Kg |
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CV8000 Partner
North America, Europe
WAKO Automation USA, Inc. 
Recommended vendor for High Content Screening integration and automation solutions.
Asia
Tekon Biotech
P.R.China, Hong Kong, Taiwan, Macao
Shanghai Genesci Medical technology Co.,Ltd.
People's Republic of China (excluding Hong Kong, Taiwan, Macao)
LBD Life Sciences China Limited.
People's Republic of China (excluding Hong Kong, Taiwan, Macao)
The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.
List of Selected Publications : CV8000, CV7000, CV6000
In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.
In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.
In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.
In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.
In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.
In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.
In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.
In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.
In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.
In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.
We have been developing a prototype of a genomic drug test support system using our CSU confocal scanner. This system administers chemical compounds that serve as potential drug candidates into living cells, which are the most basic components of all living organisms, records the changes in the amount and localization of target molecules inside cells with the CSU confocal scanner and a highly sensitive CCD camera, and processes and quantifies the captured high-resolution image data.
Brochures
Software
Product Overviews
The CV8000 features a cell incubator with an improved airtight design that facilitates the observation of cell behavior over long periods of time. In addition, the CV8000 comes with CellPathfinder, a new program that can analyze images of unlabeled cells and 3D images of samples. With these features, the CV8000 improves the efficiency of drug discovery research and biomedical research on leading-edge subjects such as iPS and ES cells.