Brain MPS Overcoming Challenges of Complex in vitro Models with Modern Tools

Advances in stem cell technology and bioengineering have enabled the development of brain microphysiological systems (bMPS)—human iPSC-derived organoids, assembloids, and organ-on-chip platforms that recapitulate essential features of neurodevelopment and function. These models offer insight into neurotoxicity, disease mechanisms, and gene–environment interactions, while overcoming key limitations of animal models. In this webinar, we will explore how modern tools—including high content and live imaging, HD-MEA recordings, and genetically engineered reporter lines—help address the challenges of complexity, reproducibility, and functional validation in bMPS. Case studies will illustrate applications from modeling synaptic disruption by metal mixtures, neuroinflammatory responses in autism-associated genotypes, to domoic acid’s impact on memory-linked neural dynamics. We will also discuss emerging efforts in standardization, immune competence, and cognitive function modeling—bringing brain MPS closer for regulatory integration and next-generation in vitro neurotoxicity testing.

Key topics include:

• Utilization of Modern Tools: High content and live imaging, HD-MEA recordings, genetically engineered reporter lines.
• Case Studies: Modeling synaptic disruption by metal mixtures, neuroinflammatory responses in autism-associated genotypes, i domoic acid’s impact on memory-linked neural dynamics.
• Efforts in Standardization: Regulatory integration and next-generation in vitro neurotoxicity testing. 

Speaker:

Lena Smirnova, PhD
Bloomberg School of Public Health, Johns Hopkins