Advanced Control Bioreactor System BR1000
Intuitive software technologies meet innovative hardware solutions to improve reproducibility and stability of cell culture conditions. The Advanced Control Bioreactor System BR1000 detects viable cell densities and glucose and lactate concentrations accurately and reliably, allowing users quickly and easily automate and refine processes within a single, complete system.
- User-friendly interface for process automation
- Advanced cell measurement software for process refinement
- Able to estimate glucose consumption in real time
- Optional remote and customization options for networked environments
In-line sensor for measuring glucose and lactate concentrations
Based on YOKOGAWA's original calibration model and instrumentation, the concentration of glucose and lactic acid in the culture medium can be measured in real-time and with high accuracy based on the absorption characteristics of near-infrared light.
In-line sensor to measure live cell density
Based on YOKOGAWA's original calibration model, the live cell density can be measured in real time from changes in the capacitance characteristics of the cell suspension. The unique calibration model also maintains high accuracy throughout the entire cell culture timeline, from seeding to high cell densities.
Soft sensor for estimating glucose consumption rates in real time
A model prediction and control algorithm processes the data obtained by the in-line sensors and outputs a highly accurate glucose consumption rate trend in real time.
Advanced control to automatically control glucose concentration
An advanced control algorithm designed specifically for cell culture provides accurate and automatic control of glucose based on in-line sensor values. Peristaltic pump systems dynamically deliver sterile glucose solution as needed to maintain the glucose set-point concentration value.
Features of the system
The instrument provides basic control of pH, temperature, and dissolved oxygen, a near-infrared sensor for monitoring cell activity, a viable cell density sensor, feed control, and data logger, all integrated into one complete system.
Extensive control functions and ease of use
Automated Cell Culture
The bioreactor system provides advanced process control driven by innovative hardware and software technologies to improve reproducibility and stability of cell culture conditions for better biopharmaceuticals.
Customization and remote support (optional)
Comes complete with a calibration model derivation tool that customizes and improves the accuracy of in-line sensors.
Provides the capability for real-time remote monitoring in a networked environment.
|Liquid delivery pump||Number of channels: 4/6|
|Stirrer control||Control range: 4-200rpm|
|Temperature control||Control range: 34-40℃|
|pH control||Control range: 6.5-7.5|
|Dissolved oxygen control||Control range: 1-5mg/L|
|In-line measurement of glucose concentration||Measurement range: 0-10.0g/L|
|In-line measurement of lactate concentration||Measurement range: 0-10.0g/L|
|In-line measurement of viable cell density||Measurement range: 1×105-1×108cells/mL|
|Other standard functions||Culture vessel (cell culture medium) weight monitor, Feed bottle weight monitor, Automation control disengagement button|
|User interface||Touch panel monitor|
|Log data file format||Custom file record format (GM10) (*.GSD *GEV)|
|Classification of laser product||Class 1 Laser Product (IEC 60825-1：2007/2014)|
|Operating environment||20 to 26℃, 20 to 80%RH without condensation, Altitude ≦ 1000m|
|Power supply||Single phase 100/115/200/230VAC, 50/60Hz, Rated power: 500W|
A critical requirement in biopharmaceutical development is the integration and automation of process equipment and analytical instruments used in the laboratory. Bioprocess labs with multiple lab-scale bioreactors often execute cultivation experiments in parallel for research or process development purposes.
As part of a collaboration between Securecell (Zurich, SW) and Yokogawa Life Science (Tokyo, Japan), this application note demonstrates the effective use of the Lucullus® Process Information Management System (Lucullus®) to assist in the control of three Advanced Control Bioreactor Systems (BR1000) to study glucose utilization of CHO cells for optimal monoclonal antibody productivity.
This paper describes how the Yokogawa BR1000 bioreactor can be used to automate through real-time sensing and improve productivity through accurate and efficient glucose control.
The integration of all essential probes, instruments, software, and delivery systems for automated delivery of media concentrations of key components can be a daunting task. We have created a default control algorithm and next generation integrated system for CHO cell expression of biologics that can be customized in as little as three optimization runs.
By employing a proprietary self-learning predictive-control algorithm, continuous glucose adjustments are made, resulting in high cell counts, better viability, and improved biologic yield. The technology can speed process development and open new possibilities for control of biopharmaceutical production.
- Soft-sensor use for real-time process control
- Software use for accurate cell models
- Dynamic predictive control for glucose feeding
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