High Speed

CSU-X1 Confocal Scanner

Get next level turnarounds with the CSU-X1 Confocal Scanner

Featuring a scanning speed of up to 2,000 fps in full frame, the system sets the industry bar for efficiency and excellence. Images are significantly brighter due to the unit’s dichroic mirrors and emission filters, and while it does feature the world’s fastest scanning speed, it never sacrifices quality or user experience. Photobleaching and phototoxicity are minimized and users can personalize the CSU-X1’s mechanisms to best suit their current requirements, selecting from 3 configurations: 1-camera model, 2-camera model, or bright field model.

  1. World's fastest scanning speed of up to 2,000 fps
  2. Microlens-enhanced Nipkow disk scanning
  3. Exchangeable dichroic mirror block and emission filters
  4. O2 emissions reduced by 40%

Principles of the Microlens-enhanced Nipkow Disk Scanning Technology

A Nipkow spinning disk containing about 20,000 pinholes and a second spinning disk containing the same number of microlens to focus excitation laser light into each corresponding pinhole are mechanically fixed with a motor, and very rapidly raster scan the field of view with about 1,000 laser beams when rotated. The pinhole and microlens pattern are arranged in our proprietary design to optimize raster scan. Multi-beam scanning with the CSU-X1 not only increases scanning speed, but also results in significantly lower photobleaching and phototoxicity, because multiple excitation needs only a low level of laser power at the specimen to fully excite fluorescence.

nipkowdisk

Details

Faster

  • The world's fastest scanning speed (up to 2,000fps in full-frame).
  • Yokogawa's proprietary filter wheel with six filter positions moves to the adjacent position at 33 ms, world's fastest speed.

Brighter

Doubled*1 excitation power efficiency with newly developed beam shaper lens, allows use of lower power lasers, and could reduce camera exposure time.

efficiency
















Triplicated*1 S/N by cutting the background noise by one third, expands capability of really low-light imaging. Significantly brighter*1 images by using most efficient dichroic mirrors and emission filters.

Optical path comfiguration


More Versatile -2nd Camera Option*1

You can either simultaneously image two different emission ranges with two cameras, or can selectively use one of the two cameras you installed which is most suitable for your current experimental requirement. For each camera port, you can select to install high-speed filter wheel (Option). In addition to standard C-mount adapter, adapters for 8X8 EMCCD camera and F-mount camera are available (Option).

More Versatile -Second Camera Port Option


More Versatile -Bright Field Path Option*2

It allows you to use one camera for both confocal imaging with the CSU-X1 and bright-field (non-confocal) imaging through the bypass light path.

More Versatile -Bright Field Path Option


More Versatile—Exchangeable mirror block and exchangeable filters

Easily exchangeable dichroic mirror block and emission filters.

More Versatile—Exchangeable mirror block and exchangeable filters

*1 It is necessary to use your choice of commercially available dichroic mirrors for simultaneous multicolor imaging.

*2 The Bright Field Option is not applicable to some microscopy set-up due to steric interference. Please inquire the applicability.

High-end model (6-position filter wheel)

High-end model (6-position filter wheel)

High-end model (12-position filter wheel)

High-end model (12-position filter wheel)

Basic model

Basic model

  Main unit *1 Main unit +
Brightfield*2
Main unit +
Secondcamera*2
Confocal scanning method Microlens-enhanced Nipkow disk scanning
Scanning speed Choice:1,500~5,000rpm (Standard)
1,500~10,000rpm (High-speed)*3
External synchronization Scan-speed synchronization through pulse signals
Input : TTL level 300Hz ~ 2KHz
Corresponding to Nipkow disk spinnig speed
1,500~10,000rpm*3
Excitation wavelangth 405~647nm
Second port - Brightfield Second camera
Dichroic mirror Option*4
Dichroic mirror switching Automatic 3CH
(Dichroic mirror block can be exchangeable)
Laser beam input AFC-connector (Polished 8 degree)
Optical fiber Yokogawa's standard Single-mode polarization-preserving fiber*5
Filter wheel
(Emission side)
Filter wheel with six filter positions
Emission filter Option*4
Operation panel Switch open / close of laser shutter
External control RS-232C interface via Control unit
Microscope mount C -mount adapter
Operating environment 15~40℃ / 20~75% RH
Power consumption
(main unit)
24VDC 1A max.
Power consumption
(AC adapter)
Input:100 up to 240VAC±10%, 50 or 60Hz±3Hz, 75Wmax.
Output:24VDC 2.5Amax.
External dimension *6 175(W)×328.5(H)×301.5(L)mm 175(W)×328.5(H)×301.5(L)mm 175(W)×328.5(H)×301.5(L)mm
Weight*7 8.9kg 11.7kg 13.0kg

*1 Supplied with a control unit ( for filter wheel ) and a filter wheel.
*2 Supplied with two control units ( one each for filter wheel and for bright field ) and a filter wheel.
*3 Option.
*4 Filters are not included ( Excitation filter , Emission filter ,Dichroic mirror ) . Please inquire as you need.
*5 Supplied with each CSU-X1 main unit.
*6 Excluding protruding parts. Including filter wheel.

  Main unit *1 Main unit +
Brightfield*2
Main unit +
Secondcamera*2
Confocal scanning method Microlens-enhanced Nipkow disk scanning
Scanning speed Choice:1,500~5,000rpm (Standard)
1,500~10,000rpm (High-speed)*3
External synchronization Scan-speed synchronization through pulse signals
Input : TTL level 300Hz ~ 2KHz
Corresponding to Nipkow disk spinnig speed
1,500~10,000rpm*3
Excitation wavelangth 405~647nm
Second port - Brightfield Second camera
Dichroic mirror Option*4
Dichroic mirror switching Automatic 3CH
(Dichroic mirror block can be exchangeable)
Laser beam input AFC-connector (Polished 8 degree)
Optical fiber Yokogawa's standard Single-mode polarization-preserving fiber*5
Filter wheel
(Emission side)
Filter wheel with 12 filter positions
Emission filter Option*4
Operation panel Switch open / close of laser shutter
External control RS-232C interface via Control unit
Microscope mount C -mount adapter
Operating environment 15~40℃ / 20~75% RH
Power consumption
(main unit)
24VDC 1A max.
Power consumption
(AC adapter)
Input:100 up to 240VAC±10%, 50 or 60Hz±3Hz, 75Wmax.
Output:24VDC 2.5Amax.
External dimension *6 258(W)×329.8(H)×213.4(L)mm 259(W)×374.3(H)×248(L)mm

309.8(W)×329.8(H)×392(L)mm

Weight*7 7.8kg 10.6kg 12.2kg

*1 Supplied with a control unit ( for filter wheel ) and a filter wheel.
*2 Supplied with two control units ( one each for filter wheel and for bright field ) and a filter wheel.
*3 Option.
*4 Filters are not included ( Excitation filter , Emission filter ,Dichroic mirror ) . Please inquire as you need.
*5 Supplied with each CSU-X1 main unit.
*6 Excluding protruding parts. Including filter wheel.

  Main unit *1 Main unit +
Brightfield*2
Main unit +
Secondcamera*2
Confocal scanning method Microlens-enhanced Nipkow disk scanning
Scanning speed Choice:1,500~5,000rpm (Standard)
1,500~5,000rpm (High-speed)*3*4
1,500~10,000rpm (High-speed)*3*4
External synchronization Option*4
Excitation wavelangth 405~647nm
Second port

-

Brightfield Second camera
Dichroic mirror Option*5
Dichroic mirror switching Manual 1CH
(Dichroic mirror block can be exchangeable)
Laser beam input AFC-connector (Polished 8 degree)
Optical fiber Yokogawa's standard Single-mode polarization-preserving fiber*6
Filter wheel
(Emission side)
-
Emission filter Option*5
Operation panel Switch open / close of laser shutter
External control -*4
Microscope mount C -mount adapter
Operating environment 15~40℃ / 20~75% RH
Power consumption
(main unit)
24VDC 1A max.
Power consumption
(AC adapter)
Input:100~240 VAC ±10% ,50 or 60Hz 、75W max.
Output : 24VDC 2.5A max.
External dimension *6 175(W)×328.5(H)×213.4(L) mm 259(W)×373(H)×213.4(L)mm

308.5(W)×328.5(H)×213.4(L)mm

Weight*7 7.5kg 10.0kg 10.0kg

*1 Supplied with a control unit ( for filter wheel ) and a filter wheel.
*2 Supplied with two control units ( one each for filter wheel and for bright field ) and a filter wheel.
*3 Option.
*4 Requires control unit for rotation speed control and external synchronization.
*5 Filters are not included ( Excitation filter , Emission filter ,Dichroic mirror ) . Please inquire as you need.
*6 Supplied with each CSU-X1 main unit.
*7 Excluding protruding parts.

Control unit・Filter Wheel

Control unit
Type for 6-position filter wheel (F1) for 12-position filter wheel (F2) for bright field (B1)
Operating condition 15~40℃ 20~75%RH
Power consumption Input : 100 up to 240VAC ± 10%, 50 or 60Hz, 200VAmax
External dimension
(mm)
213(W)×132(H)×438(L) mm
Weight( 5.2 kg 5.2 kg 5.1 kg

 

6-position filter wheel for CSU-X1
Operatimg condition 15~40℃ 20~75%RH
Power consumption -
External dimension 112(W)×226(D)×100(H)
Weight 1.9 kg

 

12-position filter wheel for CSU-X1
Operatimg condition 15~40℃ 20~75%RH
Power consumption -
External dimension 154(W)×98(D)×154(H)
Weight 2.4 kg

Comparison between target and referenced product

Comparison between target and referenced product

  Target Product: CSU-X1 Referenced product: CSU22+Filter Wheel
Energy (MJ) CO2Emissions (kg) NOxEmissions (g) SOxEmissions (g) Energy (MJ) CO2Emissions (kg) NOxEmissions (g) SOxEmissions (g)
Raw materials 2,335.5 132.5 337.1 130.1 2,610.0 148.0 374.9 147.1
Components 10,510.2 494.5 1,423.4 452.8 15,329.2 728.1 2,061.7 671.7
Machiningand assembly 1,542.9 68.7 214.1 56.8 2,259.8 100.7 313.6 83.1
Logistics 2,717.7 185.5 210.8 471.1 3,211.7 218.9 252.3 553.9
Consumption 36,713.5 1,635.9 5,094.8 1,351.5 66,087.0 2,944.8 9,171.0 1,126.4
Disposal -569.9 -31.9 -75.7 -33.6 -612.8 -34.5 -81.2 -37.4
Total 53,249.8 2,485.3 7,204.4 2,428.8 88,884.8 4,106.0 12,092.2 3,851.5
functional factor 40.1% 39.5% 40.4% 36.9%  

 Note: Value of the referenced product is calculated based on the functional factor. Functional factor:2.16

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Resources

Overview:

To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

Overview:

List of Selected Publications : CSU-X1

Overview:

Faster, Brighter, and More Versatile Confocal Scanner Unit

Industries:
Yokogawa Technical Report
2.2 MB
The CSU-X1’s dichroic mirror measures at 13mm × 15mm × 0.5mm -0.20/-.020mm, t 0.500+/-0.02mm. Click here to view additional information on the CSU-X1 Confocal Scanner Unit. ...
The external dimensions of the CSU-X1 Confocal Scanner Unit series are as follows: CSU-X1-M1: 175(W) × 328.5(H) × 213.4(L)mm CSU-X1-M2: 259(W) × 373(H) × 213.4(L)mm CSU-X1-M3: 308.5(W) × 328.5(H) × 213.4(L)mm C...
The CSU-X1’s maximum acquisition speed is 2000 fps. Learn more by clicking here.
Diagrams of the CSU-W1 Uniformizer and the CSU-X1 Confocal Scanner Unit can be found on page 14 of the General Specifications Guide. To view them, click here.

Videos

Overview:

Fast, gentle, and clear - live-cell imaging. Yokogawa's unique scanning method minimizes damage to living cells and organisms and even can capture faint/fast life phenomena. 

More than 2,500+ units scanning units sold worldwide. This fast, reliable, and accurate technology has been leading cutting-edge research and supporting researchers around the world for more than two decades.

More information: https://www.yokogawa.com/us/solutions/products-platforms/life-science/spinning-disk-confocal/

#confocal #microlens #microscope #CSU #Yokogawa #livecell

Overview:

Over past 20 years, YOKOGAWA proprietary Spinning Disk Confocal technology has been widely used as an indispensable imaging tool among top researchers. The technology enables faster live-cell observation with clearer and less photo-bleaching imaging.

News

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