Super Resolution

Yokogawa CSU-W1 SoRa Confocal Scanner Unit

Optimize results with the CSU-W1 SoRa Confocal Scanner.

Ideal for super-resolution live cell imaging, the unit allows researchers to observe images in high speed, real time without specific or lengthy sample preparations. In addition, the scanner’s spinning disk technology improves the optical limit by approximately 1.4x and almost doubles the final resolution optical limit.

New option CSU-W1 Uniformizer is released.

  • XY resolution exceeding diffraction limit
  • Ideal for super-resolution live cell imaging
  • Easy to use Technology
  • Upgradable from CSU-W1

XY resolution of approx. 120nm*1

XY resolution has been improved by approximately 1.4x the optical limit based on spinning-disk confocal technology.
Furthermore, a final resolution approximately twice the optical limit is realized through deconvolution.

NG108 cell
Image provided by Dr. Kaoru Kato, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

NG108 cell growth cone

Super-resolution live cell imaging system

Just like the CSU, high-speed real time imaging can be performed with super-resolution.
In addition, live cell imaging is possible, reducing bleaching and phototoxicity.

Movie Play

Real time live cell imaging of mitochondria (10FPS)
Image provided by Dr. Kaoru Kato, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

Real time live cell imaging of mitochondria (10FPS)

The CSU is easy to use

Super-resolution images can be observed in real time without any specific preparation of sample.
Deep position observation is made possible through optical sectioning based on confocal technology.

Movie Play

3D image

*1 For reference

Details

SoRa super-resolution principle

SoRa super-resolution principle

The image formation in regular confocal microscopes is shown as the product of the illumination PSF (point spread function) and detection PSF. If we consider the image formation on the pinhole at a position D from the optical axis, it is the product of the illumination PSF and detection PSF (as shown), and we can see that information at the position D/2 from the optical axis at the light source is transmitted. That is to say, information at the D/2 position at the light source is magnified to D on the pinhole. In order to correct this, a microlens is fitted and the individual focal points projected onto the pinhole are optically reduced by half, creating an ideal image formation.
By doing so, the resolution is made approximately equal to an ideal confocal microscope, in which the pinhole has been reduced to an infinitesimal size, producing an estimated 1.4x improvement upon regular confocal microscopes.

Reference
T. Azuma and T. Kei, “Super-resolution spinning-disk confocal microscopy using optical photon reassignment,"
Opt. Express 23, 15003-15011 (2015)

Configuration when upgrading from the CSU-W1

Configuration when upgrading from the CSU-W1

A SoRa disk can be added to your CSU-W1.
By using a magnification changer for SoRa, it’s possible to perform imaging tailored to your experimental requirements through switching between regular confocal observation and super-resolution observation.

 

1x: Confocal observation (CSU-W1)
2.8x: Super-resolution 100x objective lens
4x: Super-resolution 60x objective lens

Overview : Confocal scanner unit CSU-W1

Product specification

Product specification*1
Model 1 camera model (T1) 2 camera model (T2) Split view model (T3)
Loadable model A SoRa disk can be loaded as disk 2, and disk 1 can be selected (50μm or 25μm)
Excitation wavelength 405nm~640nm
Observation wavelength 420nm~680nm
Effective scan field of view Depends on the magnification changer for SoRa specification (see below)
External light / NIR port An external light port cannot be equipped at the same time as the intermediate magnification switcher
The NIR port cannot be used together with a SoRa disk
Magnification changer for SoRa specification
Lens-switched light path 3 light paths switched electronically  1x, 2.8x, 4.0x magnification
External dimensions 425(W)×301.1(L)×122.5(H) mm (excl. protrusions and supporting column)
Weight 13kg
Microscope connection Manufacturer-specific adapter
Scan field of view when using magnification changer for SoRa
Magnification changer for SoRa 2.8x 4.0x
Recommended objective lens 100x 60x
Effective scan field of view 61x57μm 71x67μm
Resolution: : PSF FWHM*2
XY/Z resolution (optical super-resolution) 150nm / 320nm
XY/Z resolution (after deconvolution) 120nm / 300nm

*1 Only items which differ from the CSU-W1 are shown. Specification : Confocal scanner unit CSU-W1
*2 Resolution value is for reference only.

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Intelligent Imaging Innovations

Intelligent Imaging Innovations  
(product name: Marianas and VIVO)


Nikon

Nikon Instruments Inc.  
(North and South America)

Nikon Instruments Europe B.V.  
(Europe)

Nikon Corporation  
(Asia except for Japan)
 




 

Resources

Overview:

List of Selected Publications : CSU-W1

The CSU-W1 can be upgraded with a CSU-W1 SoRa, allowing you to switch between regular confocal observation and super-resolution observation. To learn more about the CSU-W1 SoRa click here . ...

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Videos

Overview:

Fast, gentle, and clear - live-cell imaging. Yokogawa's unique scanning method minimizes damage to living cells and organisms and even can capture faint/fast life phenomena. 

More than 2,500+ units scanning units sold worldwide. This fast, reliable, and accurate technology has been leading cutting-edge research and supporting researchers around the world for more than two decades.

More information: https://www.yokogawa.com/us/solutions/products-platforms/life-science/spinning-disk-confocal/

#confocal #microlens #microscope #CSU #Yokogawa #livecell

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