Life Science

우리의 현미경 및 생명 과학 솔루션은 기초 연구에서 신약 개발, 전임상 실험에 이르기까지 다양한 응용 분야를 지원하도록 설계되었습니다.

Yokogawa의 고밀도 분석 시스템과 이중 회전 디스크 공초점 기술은 재생 의학, 신약 개발 및 정밀 의학에 사용되어 고속 및 고해상도 라이브셀 이미징을 제공합니다.

  • Spinning Disk Confocal

    듀얼 스피닝 디스크 기술의 선구자인 Yokogawa의 공초점 스캐너 장치는 광학 현미경을 변형시켜 실시간 라이브 셀 이미징 기술을 구현합니다. 

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  • High Content Analysis CellVoyager

    HCA(High-Content Analysis) 시스템은 당사의 강력한 소프트웨어와 함께 활용되어 기초 과학에서부터 복잡한 화합물 스크리닝에 이르기까지 광범위한 연구 응용 분야에서 활용됩니다. 

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  • Single-Cell Analysis Solutions Single Cellome™

    Yokogawa는 single-cell 및 live cells 에 대한 세포 핸들링 기술을 발전시키고 있습니다. SU10은 선택적이고 cell 손상을 최소화하는 자동화된 nano-point delivery기능을 제공합니다. SS2000은 공초점(confocal) 현미경 기술을 기반으로 자동으로 subcellular sampling을 제공합니다.

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  • FlowCam: Flow Imaging Microscopy

    FlowCam은 자동 이미징 기술을 바탕으로 보다 정확하고 안정적이며 신속하게 입자 분석을 가능하게 하며 이는 고객의 연구를 향상시키고 생산성을 높여 주며 품질을 보장할 수 있게 해줍니다.

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  • OpreX Informatics Manager

    Yokogawa의 OpreX Informatics Manager는 스킬 및 스케쥴링 측면에서 인적 및 물적 자원 관리를 최적화하여 전자 연구 노트(ELN) 기능을 뛰어넘는 정보 통합 솔루션입니다.

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Details

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Spinning disk confocal 원리

일반적인 confocal 현미경은 표본을 스캔하기 위해 단일 레이저 빔을 사용하는 반면, CSU는 강화 Nipkow 디스크를 이용한 마이크로렌즈 스캐닝을 이용하여 약 1,000개의 레이저 빔으로 표본을 스캔합니다. 즉, CSU는 1,000배 더 빠르게 스캔할 수 있습니다.

마이크로렌즈가 정렬되어 있는 디스크와 Nipkow 디스크를 함께 결합하여 사용함으로써 광효율을 획기적으로 향상시키는데 성공하였으며 이는 살아있는 세포의 실시간 공초점 이미지를 성공적으로 구현했습니다.

확장되고 평행으로 조준된 레이저 빔은 약 20,000개의 마이크로렌즈가 포함된 상부 디스크(microlens array disk)를 비춥니다. 각 마이크로렌즈는 레이저 빔을 해당 핀홀에 집중시켜 핀홀 어레이 디스크(Nipkow disk)에 배치된 핀홀을 통해 레이저 강도를 효과적으로 증가시킵니다.

마이크로렌즈를 사용하면 핀홀 디스크 표면에서 레이저 빛의 후방 산란을 크게 줄일 수 있으므로 공초점 이미지의 신호 대 잡음비(S/N)를 현저히 증가시킵니다.

각각의 핀홀을 통과한 약 1,000개의 레이저 빔이 대물렌즈의 조리개를 채운 후 초점면에 초점을 맞춥니다. 검체에서 생성된 형광은 대물렌즈에 의해 캡처되어 핀홀 디스크로 다시 초점이 맞추어 지고, 동일한 홀을 통해 전송되어 초점이 맞지 않는 신호를 제거하고 마이크로렌즈 어레이 디스크와 Nipkow 디스크 사이에 위치한 이색성 거울((dichroic mirror)에 의해 편향된 이 반사된 레이저에서 형광 신호를 분리한 후 발광 필터를 통과하여 접안렌즈 또는 카메라의 이미지로 포커싱 됩니다.

마이크로렌즈 어레이 디스크와 Nipkow 디스크가 서로 물리적으로 고정된 채 회전하면서 시야 전체를 고속으로 스캔함으로 CSU 헤드의 접안렌즈를 통해 공초점 형광 이미지를 실시간으로 볼 수 있게 됩니다.

기존의 단일 포인트 스캐닝에 비해 CSU에 의한 다중 빔 스캐닝은 단위 면적당 매우 낮은 수준의 광 강도를 필요로 하므로 live cell의 광 표백 및 광독성을 크게 감소시킬 수 있습니다.

Spinning Disk Confocal

microlens / fastsacnning / minimal photo bleach / high resolution

Microlens-enhanced Nipkow Disk Technology

Microlens-enhanced Nipkow Disk Technology

Comparison of scanning method

point scanning

Point Scanning
1 line scan time=1[ms]
1000 lines/image
Scan lines=1000 [lines]
1×1000=1000 [ms]

disk scanning

Disk Scanning by CSU
Rotation Speed=10000 [rpm]=41.7[rps]
30°Rotation/image
1÷( 41.7×30/360 )= 0.5 [ms]

 

 

January 16,2019

SLAS 2019

February 4-6, 2019
Walter E. Washington Convention Center
Booth No. 214

We will exhibit high content analysis system "CellVoyager".

Link to products
CellVoyagerTMCV8000 High-throughput Cytological Discovery System
Confocal Quantitative Image Cytometer CQ1
High Content Analysis Software CellPathfinder

*Poster presentation is planned. Details will be posted as soon as it is decided.

Find out more about SLAS 2019

October 24,2018

ASCB/EMBO 2018

December 9-11, 2018
Booth No. 845

-Tech talks-

December 9, 3:00-4:00 pm – Theater 2, Learning Center

Super Resolution Confocal Scanner Unit CSU-W1 Sora

Presenter: Takuya Azuma: Chief designer of CSU-W1 Sora,
Yoshitaka Sekizawa: Product manager of CSU-W1 Sora

Yokogawa will introduce our brand-new product “CSU-W1 SoRa.” This is a spinning disk based super resolution confocal scanner unit. In this talk, we will introduce features and principles of this product and we will show beautiful image samples taken by “CSU-W1 SoRa”. Features of “CSU-W1 SoRa”: 1) XY resolution of approx. 120nm. XY resolution has been improved by approximately 1.4x the optical limit based on spinning-disk confocal technology. Furthermore, a final resolution approximately twice the optical limit is realized through deconvolution. 2) Ideal for super-resolution live cell imaging. Just like the CSU, high-speed real time imaging can be performed with super-resolution. In addition, live cell imaging is possible, reducing bleaching and phototoxicity. 3) The CSU is easy to use. Super-resolution images can be observed in real time without any specific preparation of sample. Deep position observation is made possible through optical sectioning based on confocal technology. 4) Upgradable from CSU-W1. If you already have CSU-W1, you can add SoRa disk.

Find out more about ASCB/EMBO 2018

September 14,2018

Sales release : High Content Analysis Software CellPathfinder

 Click Here For More Info

July 27,2018

Sales release : High-speed Super resolution Confocal Scanner CSU-W1 SoRa

 Click Here For More Info

June 11,2018

2018 SLAS Europe

March 01,2018

Sales release : High Content Data Management System CellLibrarian

 Click Here For More Info

December 29,2017

SLAS 2018

February 3-7, 2018

Find out more about SLAS 2018

December 29,2017 Sales news : The Discontinuation of CellVoyagerTM CV7000S High-throughput Cytological Discovery System
September 05,2017

Sales release : CellVoyagerTMCV8000 High-throughput Cytological Discovery System

 Click Here For More Info

January 19,2017

SLAS High-Content Screening Conference 2017

Find out more about SLAS High-Content Screening Conference 2017

April 04,2016

Poster presentation in 3D Cell Culture 2016, 19-21 April 2016, Konzerthaus Freiburg/Germany

Yokogawa Electric Corporation will present data obtained by our confocal image cytometer CQ1 in “3D Cell Culture 2016: How close to ‘in vivo’ can we get? Models, Application & Translation”. The poster will show the results of 3D live cell imaging and analysis of the migration and the network formation of HUVEC cells in a multilayered cell sheet. The results demonstrate that CQ1 is an excellent research tool in the field such as regenerative medicine and drug discovery screening.

*Data were provided from BioProcess Systems Engineering Lab., Dept.Biotech., Grad. Sch. Eng., Osaka University.

Poster presentation in 3D Cell Culture 2016

February 10,2016 Yokogawa Concludes Distribution Agreement with Optec, LLC for Sale of Confocal Quantitative Image Cytometer CQ1 at the markets of OPTEC activity
October 01,2015 Sales release : Label-free Morphological Analysis Software CellActivision

 

자료실

Overview:

Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression

Overview:

Faster, Deeper, and Clearer -in vivo molecular imaging technology-

Overview:

Discovering the Basic Principles of Life through the Live Imaging of C. elegans

Overview:

Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.

Overview:

New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!

Overview:

Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.

Overview:

Use of the spinning disk confocal at the Harvard Medical School microscopy core.

Overview:

Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.

Overview:

On-site manipulation of protein activities: Understanding intricate cell signaling pathways.

Application Note
Overview:

Comparison between CSU and conventional LSM in 4D movies.

Application Note
Application Note
Overview:

To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

Application Note
Overview:

CV1000 clears the hurdle in Live Cell Imaging
All-in-one Live cell imaging solution

Overview:

The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.

Application Note
Application Note
Overview:

Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.

업종:
Overview:

SU10 is a novel technology that enables the delivery of target substances directly into cells (nucleus or cytoplasm) using a "nano" pipette made of a glass capillary with an outer tip diameter of tens of nanometers.

Application Note
Overview:

The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

Application Note
Overview:

List of Selected Publications : CSU-W1

Overview:

List of Selected Publications : CQ1

Overview:

List of Selected Publications : CSU-X1

Overview:

List of Selected Publications : CV8000, CV7000, CV6000

Overview:
  • Colony Formation
  • Scratch Wound
  • Cytotoxicity
  • Neurite Outgrowth
  • Co-culture Analysis
  • Cell Tracking
업종:
Overview:

Faster, Brighter, and More Versatile Confocal Scanner Unit

업종:
Application Note
Overview:

Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System

Application Note
Overview:

Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer

Application Note
Overview:

Wide and Clear
Confocal Scanner Unit

업종:
Yokogawa Technical Report
2.2 MB
Overview:

This "Tutorial" provides overview of this software, from installation through data analysis.

Overview:

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

Overview:

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

Overview:

In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.

Overview:

In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

Overview:

In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.

Overview:

In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.

Overview:

In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.

Overview:

In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.

Overview:

In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.

Overview:

In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.

Downloads

동영상

Product Overview
Overview:

The Yokogawa business vision states that the company endeavors to achieve Net-zero emissions, ensure the Well-being of all, and make a transition to a Circular Economy by 2050. 

Overview:

YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.

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