With its compact footprint and lightweight, benchtop design, there is no need for a darkroom or specialized setup for the CellVoyager CQ1. The unit provides rich feature extractions, facilitating sophisticated cellular image analysis, and while the Nipkow Spinning Disk Confocal Technology allows high speed scanning, it reduces phototoxicity and photobleaching.
- Easy to use, intuitive, simple acquisition software increases productivity
- Compact design contains multiple, fully integrated functions
- CSU W-1 confocal spinning disk technology results in higher scanning speeds and higher quality images
- Systems open platform allows for integration for laboratory automation
- Supports the CellPathfinder high content analysis system
Enables measurement of spheroids, colonies, and tissue sections
- No need to remove cells from the culture dish, in contrast to traditional flow cytometry
- Nipkow spinning disk confocal technology allows high-speed yet gentle 3D image acquisition
- Rich feature extraction to facilitate sophisticated cellular image analysis
- Wide field of view and tiling capability enables easy imaging of large specimen
Enables analysis of time-lapse and live-cell
- High precision stage incubator and low phototoxicity of our confocal makes the analysis of time-lapse and live-cell are possible
- Max. 20fps option for fast time-lapse*1
High-quality image and similar operability to a traditional flow cytometer
- Integration with CellPathfiner software can provide powerful analysis displayed in real-time with image acquisition
- Usable high-quality image as confocal microscope image.
- Interactive graphs make it easy to trace back the data points
- Connectable with external systems via handling robot*2
- Expandable to the integrated system as image acquisition and quantification instrument
- FCS/CSV/ICE data format readable by third-party data analysis software
- A variety of cell culture and sample dishes are applicable
Compact footprint, lightweight bench-top device; no need for a darkroom
*2 Contact to CQ1 partner for more information
Multiple functions fully integrated into a compact box
Compact design contains fully integrated functions to offer an easy-to-handle confocal imaging system, without a need for complicated system integration. You only need to set a sample and run the software. A user-friendly interface and versatile functions support your measurement and analysis.
Principles of the Microlens-enhanced Nipkow Disk Scanning Technology
A Nipkow spinning disk containing about 20,000 pinholes and a subsidiary spinning disk containing the same number of microlenses to focus excitation laser light into each corresponding pinhole are mechanically fixed on a motor, and very rapidly rotated. As a result, a high-speed raster scan of the excitation lights on the specimen can be achieved. The pinhole and microlenses are arranged on each disk in our proprietary design to optimize the raster scan. Multi-beam scanning not only increases scanning speed but also results in significantly lower photobleaching and phototoxicity because multi-beam excitation needs only a low level of laser power on the specimen to fully excite fluorescence.
Example of setup
|Optics||Microlens enhanced dual wide Nipkow disk confocal,
Phase contrast (Optional add-on)
|Laser/Filter||Laser: Choose 2-4 lasers from 405/488/561/640nm,
10-position Filter wheel (built-in)
|Camera||sCMOS 2560×2160pixel, 16.6×14.0mm|
|Objective lens||Max.6 lenses
(Dry: 2x, 4x, 10x, 20x, 40x Long working distance: 20x, 40x
Phase contrast: 10x, 20x )
|Sample vessel||Microplate (6, 24, 96, 384 well), Slide glass,
Cover glass chamber*1, Dish (35, 60mm*1)
|XY stage||High-precision XY stage, designated resolution 0.1µm|
|Z focus||Electric Z motor, designated resolution 0.1µm|
|Autofocus||Laser autofocus, Software autofocus|
|Feature data||Number of cells/cellular granules, Intensity, Volume, Surface area, Area, Perimeter, Diameter, Sphericity, Circularity, etc|
|Data format||Image: 16bit TIFF file (OME-TIFF), PNG
Numerical data : FCS, CSV, ICE
|Workstation||Measurement and analysis workstation|
|Size/weight||Main unit: 600×400×298mm 38kg
Utility box: 275×432×298mm 18kg
|Environment||15 - 30oC、20 - 70％RH No condensation|
|Power consumption||Main unit and Utility box: 100-240VAC 800VAmax, Workstation: 100-240VAC 650VAmax|
*1 Under development *2 Display is not included with CQ1 system
- Preset analysis menus for a variety of applications
- Flexible graph functions to display analysis results
- Direct link between chart and object image
The software learns the features of the sample objects collected by users.
Digital phase contrast function is a powerful tool to analyze unstained bright field samples.
Simple, intuitive measurement procedures
Compare CQ1, Flourescent Imaging, and Flow Cytometry
|CQ1||General fluorescent imaging||Flow cytometry|
|Cell removal/suspension treatment||Not necessary||Not necessary||Necessary|
|Cell image confirmation||Possible||Possible||Not possible|
|Display feature data and graphs in real-time with imaging||Possible||Depends on devices||Possible|
|3D data measurement||Possible||Not possible||Not possible|
|Time-lapse||Possible||Not possible||Not possible|
PhenoVista Biosciences is the leading provider of custom, imaging-based, phenotypic assay services. With a collaborative and scientifically driven project design and management approach, PhenoVista has a proven track record of delivering high-quality data from robust and scalable assays. PhenoVista’s key advantage lies in the ability of their industry-trained scientists to combine world-class understanding of diverse biological systems with cutting-edge quantitative imaging to deliver clear, actionable output data.
Fluorescent ubiquitination-based cell cycle indicator (Fucci) is a set of fluorescent probes which enables the visualization of cell cycle progression in living cells.
The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.
The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.
List of Selected Publications : CQ1
Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.
Yokogawa's CQ1 open platform integrates seamlessly with Advanced Solutions BioAssemblyBot® 400. With laboratory automation becoming a standard in research, Yokogawa's high content confocal system's ability to work with robots like Advanced Solutions' BioAssemblyBot® 400 is essential to advancing laboratory automation.
In this webinar, Professor Jonny Sexton discusses a pipeline, developed in the Sexton lab, for the quantitative high-throughput image-based screening of SARS-CoV-2 infection to identify potential antiviral mechanisms and allow selection of appropriate drug combinations to treat COVID-19. This webinar presents evidence that morphological profiling can robustly identify new potential therapeutics against SARS-CoV-2 infection as well as drugs that potentially worsen COVID-19 outcomes.
Generating translatable high-content imaging data from physiologically-relevant cell models, including 2D and 3D structures, is extremely valuable for drug discovery and pre-clinical research. In this webinar, James Evans, CEO of PhenoVista Biosciences presents case studies on how Yokogawa’s Benchtop CQ1 Confocal System can improve throughput and standardize processes for complex 3D cell-based phenotypic assays.
Key learning objectives:
- Strategies for designing and implementing high-content screening assays
- Approaches for deciding between 2D and 3D model systems
3D imaging experts from Yokogawa and Insphero have come together to provide helpful tips and tricks on acquiring the best 3D spheroid and organoid imaging. This webinar focuses on sample preparation, imaging, and analysis for both fixed and live cells in High Content Screening assays. The experts also discuss automated tools that can help researchers understand the large volume of data in these High Content Imaging Analysis Systems.
In the last few decades, the pharmaceutical industry has transformed people’s lives. However, the development of new drugs is becoming increasingly difficult and a paradigm shift in the drug discovery workflow is required to reduce attrition and transform conventional drug screening assays into translatable analytical techniques for the analysis of drugs in complex environments, both in-vitro and ex-vivo. The ability to visualize unlabelled compounds inside the cell at physiological dosages can offer valuable insight into the compound behavior both on and off-target.
SiLC-MS is a semi-automated methodology that allows the collection of intracellular contents using a modified CQ1 imaging system developed by Yokowaga. The instrument is equipped with a confocal microscope that allows bright field imaging as well as fluorescence imaging with 4 lasers (405, 488, 561, and 640 nm). Sampling is performed using the tips developed by Professor Masujima (1-4).
In this study, we show the applicability of the SiLC-MS technology to drug discovery, as it is crucial to identify compound and its metabolites when incubated in a mammalian cell at a therapeutic dose. We report on the validation studies performed using the SiLC-MS platform, in these validation studies we assess the ability to distinguish different cell types based on their metabolomic fingerprint, furthermore, we have also evaluated if this assay was sensitive enough to detect drugs intracellularly.
Presenter: Carla Newman, Scientific Leader (Celluar Imaging and Dynamics), GSK
Visualizing the complex spatiotemporal dynamics of human stem cells as they proliferate and make cell fate decisions is key to improving our understanding of how to robustly engineer differentiated tissues for therapeutic applications.
In this webinar, Dr. Rafael Carazo Salas will describe multicolor, multiday high-content microscopy pipelines that his group has recently developed to visualize the dynamical cell fate changes of human Pluripotent Stem Cells (hPSCs).
- Visualizing how human Pluripotent Stem Cells (hPSCs) proliferate and undergo early differentiation in vitro, by high content microscopy
- Learning about experimental and computational pipelines that enable monitoring single-cell fate dynamics
- Learning about novel “live” reporters of hPSC cell fate
Rafael Carazo Salas, PhD
Professor, School of Cellular and Molecular Medicine
University of Bristol
This webinar highlights Yokogawa’s High Content Solutions, the benchtop confocal CellVoyager CQ1, and CellVoyager CV8000. Utilizing Yokogawa’s dual-wide microlens spinning disk confocal technology, these automated HCA systems provide remarkable image quality while increasing your output. This frees up time to complete other research activities. Also, recent additions to the CSU-W1 confocal upgrade is discussed. The SoRa, a super-resolution solution, and the Uniformizer, an image flattening device. Both of which can be added to the lightpath of your CSU-W1-enhanced microscope.
Introduction to Yokogawa
SoRa for CSU-W1 super-resolution with confocal
Two high content instruments from Yokogawa: The CQ1 and the CV8000
Dan J. Collins, Applications Scientist, Yokogawa Life Science
Dr. Sexton discusses high content screening for phenotypic-based drug discovery and development using Yokogawa technologies. This webinar presents the methodology behind acquiring good images that are able to leverage the three-dimensionality of different cell systems. His assays include 3D models such as organoids and spheroids.
In this webinar, you will discover:
- How to identify when 2D or 3D methods are required to achieve desired results.
- How to leverage your High Content Imaging Systems to get optimal signals and backgrounds.
- Techniques that are used to improve cell observation yield and statistical distributions of morphological features.
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